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10.11.2017 | Original Contribution | Ausgabe 8/2018

European Journal of Nutrition 8/2018

Co-supplementation of isomalto-oligosaccharides potentiates metabolic health benefits of polyphenol-rich cranberry extract in high fat diet-fed mice via enhanced gut butyrate production

Zeitschrift:
European Journal of Nutrition > Ausgabe 8/2018
Autoren:
Dhirendra Pratap Singh, Shashank Singh, Vandana Bijalwan, Vijay Kumar, Pragyanshu Khare, Ritesh Kumar Baboota, Paramdeep Singh, Ravneet Kaur Boparai, Jagdeep Singh, Kanthi Kiran Kondepudi, Kanwaljit Chopra, Mahendra Bishnoi
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s00394-017-1561-5) contains supplementary material, which is available to authorized users.

Abstract

Purpose

Cranberries are a rich source of polyphenolic antioxidants. Purified sugars or artificial sweeteners are being added to cranberry-based food products to mask tartness. Refined sugar and artificial sweeteners intake modulate gut microbiota and result in metabolic complications. We evaluated effects of isomalto-oligosaccharides (IMOs; sweet tasting non-digestible oligosaccharides) with cranberry extract (CRX) on high fat diet (HFD)-induced metabolic alterations in mice.

Methods

Male Swiss albino mice were fed normal chow or HFD (58% fat kcal), and were administered either CRX (200 mg/kg) alone or in combination with IMOs (1 g/kg). Cecal short-chain fatty acids, abundances of selected (1) butyrate producing, (2) metabolically beneficial, and (3) selective lipopolysaccharides producing gram negative gut bacteria were studied. Further, gut-related histological, biochemical, genomic changes along with circulating pro-/anti-inflammatory markers and systemic obesity-associated metabolic changes were studied.

Results

Co-supplementation of CRX and IMOs significantly improved cecal SCFAs, especially butyrate levels, selected butyrate-producing bacteria (clostridial cluster XIVa bacteria) and butyrate kinase expression in HFD-fed mice. The combination also significantly improved gut beneficial bacterial abundance, gut histology and related changes (colon mucin production, gut permeability) as compared to individual agents. It also prevented HFD-induced systemic and tissue inflammation, glucose intolerance and systemic obesity-associated metabolic changes in adipose tissue and liver. The combination of CRX and IMOs appeared more effective in the prevention of HFD-induced gut derangements.

Conclusion

Combination of CRX and IMOs could be advantageous for normalization of metabolic alterations seen in diet-induced obesity via beneficial modulation of gastrointestinal health.

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Zusatzmaterial
Supplementary material 1 (DOCX 15 KB)
394_2017_1561_MOESM1_ESM.docx
Supplementary material 2 (DOCX 23 KB)
394_2017_1561_MOESM2_ESM.docx
Supplementary material 3 (DOCX 22 KB)
394_2017_1561_MOESM3_ESM.docx
Online resource 4: Effect of CRX and its combination with IMOs on lipid metabolism. Mice were fed either with NPD or HFD for 12 weeks. CRX (200 mg/kg) and its combination with IMOs (1 g/kg) were administered per orally to mice fed on HFD. A–D) serum lipid parameters (n= 5 – 6); E) expression of key lipid metabolism genes in vWAT (n=5); F–I) liver lipid parameters (n=5); J) expression of key lipid metabolism genes in liver (n=5); K) representative photomicrographs of oil red-O stained muscle sections (scale=100 µm). Values are expressed as mean ± SEM. Intergroup variations were assessed by one way ANOVA followed by Tukey’s multiple comparison test. * P < 0.05 versus NPD, ** P < 0.01 versus NPD, *** P < 0.001 versus NPD, # P < 0.05 versus HFD, ## P < 0.01 versus HFD, ### P < 0.001 versus HFD, † P < 0.05 versus HFD + CRX (TIF 5650 KB)
394_2017_1561_MOESM4_ESM.tif
Online resource 5: Effect of CRX and its combination with IMOs on feeding behaviour and activity. Mice were fed either with NPD or HFD for 12 weeks. CRX (200 mg/kg) and its combination with IMOs (1 g/kg) were administered per orally to mice fed on HFD. Average feed and water intake and urine output was measured in metabolic cages (n=4 mice/cage and n= 10 mice/group). Total locomotor activity (rearing plus ambulatory movement) was measured in a photoactometer. All the measurements were taken thrice in a month and average values are presented with SEM. Two-way ANOVA followed by Tukey’s post-hoc test was applied to asses any intergroup variations. * P < 0.05 versus NPD, *** P < 0.001 versus NPD, # P < 0.05 versus HFD, ## P < 0.01 versus HFD (TIF 174 KB)
394_2017_1561_MOESM5_ESM.tif
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