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27.01.2022 | Original Article

CO₂ pneumoperitoneum effects on proliferation and apoptosis in two different neuroblastoma cell lines

verfasst von: Monica Currò, Salvatore Arena, Angela Simona Montalto, Patrizia Perrone, Donatella Di Fabrizio, Maria Paola Bertuccio, Carmelo Mazzeo, Daniela Caccamo, Riccardo Ientile, Carmelo Romeo, Pietro Impellizzeri

Erschienen in: Pediatric Surgery International | Ausgabe 3/2022

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Abstract

Purpose

The proto-oncogene MYCN is considered a transcription factor involved in the regulation of neuroblastoma (NB) cell biology. Since minimally invasive-surgery represents a debated treatment of NB, we investigated CO₂ effects on proliferative activity and apoptotic pathway in two NB cell lines, SH-SY5Y (MYCN-non-amplified) and IMR-32 (MYCN-amplified).

Methods

SH-SY5Y and IMR-32 were exposed to CO₂ (100%) at a pressure of 15 mmHg for 4 h and then moved to normal condition for 24 h. Cell proliferation, caspase 3 activity and transcript levels of BAX, BCL-2, cyclin B, cyclin D and MMP-2 were evaluated.

Results

CO₂ exposure caused a decrease in cell proliferation associated to increases in BAX/BCL-2 ratio and caspase 3 activity in SH-SY5Y, while opposite effects have been found in IMR-32. CO₂ exposure induced a decrease of cyclin B1 in SH-SY5Y, while an increase in cyclin B1 and D1 was observed in IMR-32. A slight up-regulation of MMP-2 expression in SH-SY5Y and a significant increase of 2.2 folds in IMR-32 was observed (p < 0.05).

Conclusions

Our results suggest that CO₂ exposure may cause different effects on various NB cell lines, likely due to MYCN amplification status. Further in vitro and in vivo studies are needed to highlight the role of laparoscopy on NB behaviour.

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Titel: CO2 pneumoperitoneum effects on proliferation and apoptosis in two different neuroblastoma cell lines
verfasst von: Monica Currò
Salvatore Arena
Angela Simona Montalto
Patrizia Perrone
Donatella Di Fabrizio
Maria Paola Bertuccio
Carmelo Mazzeo
Daniela Caccamo
Riccardo Ientile
Carmelo Romeo
Pietro Impellizzeri
Publikationsdatum: 27.01.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 3/2022
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-022-05063-9

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CO₂ pneumoperitoneum effects on proliferation and apoptosis in two different neuroblastoma cell lines