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Lasers in Medical Science

Erschienen in:

06.01.2018 | Original Article

Combination of MPPa-PDT and HSV1-TK/GCV gene therapy on prostate cancer

verfasst von: Liming Liang, Wenxiang Bi, Weiwen Chen, Yani Lin, Yuanyuan Tian

Erschienen in: Lasers in Medical Science | Ausgabe 2/2018

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Abstract

We combined pyropheophorbide-a methyl ester, photodynamic therapy (MPPa-PDT), 670 ± 10 nm, 4 mW/cm², with herpes simplex virus type 1 thymidine kinase/ganciclovir (HSV1-TK/GCV) to improve the therapeutic effect. We built HSV1-TK expression vector GV230-TK and we observed a bright green fluorescence under fluorescence microscope. It indicated the recombinant plasmid was transfected into PC-3M prostate cancer cells successfully. As the abundant glucose-regulated protein 78 (GRP78) promoter in PC-3M cells can cause active expression of HSV1-TK, cell protein was collected for western blot to determine the expression of HSV1-TK. In CCK-8 assay (n = 6), the cell survival rate of combined treatment group was about 10%, less than that of pure MPPa-PDT group (23%) and pure HSV1-TK/GCV group (35%) (t test, P < 0.05). Flow cytometry was used to measure the cytotoxicity; the apoptosis rate of combined treatment group was about 38%, higher than that of pure MPPa-PDT group (about 22%) and pure HSV1-TK/GCV group (about 19%). The results showed that the combination of the two treatments can effectively improve the cytocidal effect in PC-3M cells.

Teng L, Nakada M, Zhao SG, Endo Y, Furuyama N, Nambu E, Pyko IV, Hayashi Y, Hamada JI (2011) Silencing of ferrochelatase enhances 5-aminolevulinic acid-based fluorescence and photodynamic therapy efficacy. Br J Cancer 104:798–807CrossRefPubMedPubMedCentral

Giannelli M, Landini G, Materassi F, Chellini F, Antonelli A, Tani A, Nosi D, Zecchi-Orlandini S, Rossolini GM, Bani D (2017) Effects of photodynamic laser and violet-blue led irradiation on Staphylococcus aureus biofilm and Escherichia coli lipopolysaccharide attached to moderately rough titanium surface: in vitro study. Lasers Med Sci 32:857–864CrossRefPubMed

Zhang Q, Liu Z, Hou J, Wang S, Liu C, Wei M, Liu Y, Shao Y (2016) Improved safety of a replication-competent poxvirus-based HIV vaccine with the introduction of the HSV-TK/GCV suicide gene system. Vaccine 34:3447–3453CrossRefPubMed

Pourzadegan F, Shariati L, Taghizadeh R, Khanahmad H, Mohammadi Z, Tabatabaiefar MA (2016) Using intron splicing trick for preferential gene expression in transduced cells: an approach for suicide gene therapy. Cancer Gene Ther 23:7–12CrossRefPubMed

Fu Y, Wey S, Wang M, Ye R, Liao CP, Roy-Burman P, Lee AS (2008) Pten null prostate tumorigenesis and AKT activation are blocked by targeted knockout of ER chaperone GRP78/BiP in prostate epithelium. Proc Natl Acad Sci 105:19444–19449CrossRefPubMedPubMedCentral

Ni M, Zhang Y, Lee AS (2011) Beyond the endoplasmic reticulum: atypical GRP78 in cell viability, signalling and therapeutic targeting. Biochem J 434:181–188CrossRefPubMedPubMedCentral

Azatian A, Yu H, Dai W, Schneiders FI, Botelho NK, Lord RV (2009) Effectiveness of HSV-tk suicide gene therapy driven by the Grp78 stress-inducible promoter in esophagogastric junction and gastric adenocarcinomas. J Gastrointest Surg 13:1044–1051CrossRefPubMed

Hannani D, Merlin E, Gabert F, Laurin D, Deméocq F, Chaperot L, Kanold J, Plumas J (2010) Photochemotherapy induces a faster apoptosis of alloreactive activated T cells than of nonalloreactive resting T cells in graft versus host disease. Transplantation 90:1232–1238CrossRefPubMed

King GD, Kroeger KM, Bresee CJ, Candolfi M, Liu C, Manalo CM, Muhammad AK, Pechnick RN, Lowenstein PR, Castro MG (2008) Flt3L in combination with HSV1-TK-mediated gene therapy reverses brain tumor-induced behavioral deficits. Mol Ther 16:682–690CrossRefPubMedCentral

10.

Yin X, Yu B, Tang Z, He B, Ren J, Xiao X, Tang W (2013) Bifidobacterium infantis-mediated HSV-TK/GCV suicide gene therapy induces both extrinsic and intrinsic apoptosis in a rat model of bladder cancer. Cancer Gene Ther 20:77–81CrossRefPubMed

11.

Jiang W, Xu CJ, Shao ZM, Zhou WJ, Ye B, Tian PK, Zhu JD, Gu JR (2005) Enhanced efficiency and specificity of ovarian cancer gene therapy in rats with a novel nonviral gene delivery system (GE7) via intraovarian artery perfusion approach. Cancer Gene Ther 12:810–817CrossRefPubMed

12.

Kang NH, Hwang KA, Yi BR, Lee HJ, Jeung EB, Kim SU, Choi KC (2012) Human amniotic fluid-derived stem cells expressing cytosine deaminase and thymidine kinase inhibits the growth of breast cancer cells in cellular and xenograft mouse models. Cancer Gene Ther 19:412–419CrossRefPubMed

13.

Ghoodarzi R, Changizi V, Montazerabadi AR, Eyvazzadaeh N (2016) Assessing of integration of ionizing radiation with Radachlorin-PDT on MCF-7 breast cancer cell treatment. Lasers Med Sci 31:213–219CrossRefPubMed

14.

Yan W, Zhou Y, Zhou Z, Ji Z, Li H (2017) Padeliporfin vascular-targeted photodynamic therapy versus active surveillance in men with low-risk prostate cancer. Lancet Oncol 18:e187CrossRefPubMed

15.

Cengel KA, Simone CB 2nd, Glatstein E (2016) PDT: what’s past is prologue. Cancer Res 76:2497–2499CrossRefPubMedPubMedCentral

16.

Culver KW, Ram Z, Wallbridge S, Ishii H, Oldfield EH, Blaese RM (1992) In vivo gene transfer with retroviral vector-producer cells for treatment of experimental brain tumors. Science 256:1550–1552CrossRefPubMed

17.

Leinonen HM, Ruotsalainen AK, Määttä AM, Laitinen HM, Kuosmanen SM, Kansanen E, Pikkarainen JT, Lappalainen JP, Samaranayake H, Lesch HP, Kaikkonen MU, Ylä-Herttuala S, Levonen AL (2012) Oxidative stress-regulated lentiviral TK/GCV gene therapy for lung cancer treatment. Cancer Res 72:6227–6235CrossRefPubMed

18.

Shi Y, Wang J, Bai Z, Li Y, Qiu L, Zhai B, Zhang F, Yang X (2016) Radiofrequency hyperthermia-enhanced herpes simplex virus-thymidine kinase/ganciclovir direct intratumoral gene therapy of esophageal squamous cancers. Am J Cancer Res 6:2054–2063PubMedPubMedCentral

19.

Billat PA, Ossman T, Saint-Marcoux F, Essig M, Rerolle JP, Kamar N, Rostaing L, Kaminski H, Fabre G, Otyepka M, Woillard JB, Marquet P, Trouillas P, Picard N (2016) Multidrug resistance-associated protein 4 (MRP4) controls ganciclovir intracellular accumulation and contributes to ganciclovir-induced neutropenia in renal transplant patients. Pharmacol Res 111:501–508CrossRefPubMed

20.

Gentry BG, Boucher PD, Shewach DS (2006) Hydroxyurea induces bystander cytotoxicity in cocultures of herpes simplex virus thymidine kinase-expressing and nonexpressing HeLa cells incubated with ganciclovir. Cancer Res 66:3845–3851CrossRefPubMed

21.

Zeng ZJ, Xiang SG, Xue WW, Li HD, Ma N, Ren ZJ, Xu ZJ, Jiao CH, Wang CY, Hu WX (2014) The cell death and DNA damages caused by the Tet-On regulating HSV-tk/GCV suicide gene system in MCF-7 cells. Biomed Pharmacother 68:887–892CrossRefPubMed

Titel: Combination of MPPa-PDT and HSV1-TK/GCV gene therapy on prostate cancer
verfasst von: Liming Liang
Wenxiang Bi
Weiwen Chen
Yani Lin
Yuanyuan Tian
Publikationsdatum: 06.01.2018
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 2/2018
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2331-6

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Combination of MPPa-PDT and HSV1-TK/GCV gene therapy on prostate cancer

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

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