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22.11.2016 | Original Article

Combined effects of multiple linked loci on pairwise sibling tests

verfasst von: Tomonori Tamura, Motoki Osawa, Yu Kakimoto, Eriko Ochiai, Takanori Suzuki, Takashi Nakamura

Erschienen in: International Journal of Legal Medicine | Ausgabe 1/2017

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Abstract

The advanced multiplex STR system, PowerPlex Fusion, includes four linked locus pairs. The conventional Identifiler system has one pair of linked loci. Therefore, sibling tests conducted using the advanced system might be more affected by linkage than those conducted using the conventional system. This study simulated single and combined effects of the four linked locus pairs on pairwise sibling tests. Simulated genotypes of 100,000 pairs of full siblings and nonrelatives were constructed according to allele frequencies of the Japanese population. The single linkage effect was evaluated for simulated genotype data by calculating both the likelihood ratio accounting for the linkage between two loci and the likelihood ratio ignoring the linkage. The combined effect was obtained by multiplication of the respective single effects. Furthermore, we investigated the possibility that ignoring the linkage affects subject classification by introducing a scale of the likelihood ratio into sibling tests. The single effect in the Identifiler analysis was 0.645–1.746 times if the linkage was ignored. Overestimations and underestimations were predictable from the identical-by-state status at two linked loci. The combined effect in the PowerPlex Fusion analysis was 0.217–7.390 times. Ignoring the linkage rarely caused a false conclusive or inconclusive result, even from PowerPlex Fusion analysis. Application of the advanced system improved sibling tests considerably. The additional examined loci were more beneficial than the adverse effect of the linkage derived from the four linked locus pairs.
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Metadaten
Titel
Combined effects of multiple linked loci on pairwise sibling tests
verfasst von
Tomonori Tamura
Motoki Osawa
Yu Kakimoto
Eriko Ochiai
Takanori Suzuki
Takashi Nakamura
Publikationsdatum
22.11.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
International Journal of Legal Medicine / Ausgabe 1/2017
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-016-1491-4

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