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15.04.2018 | Original Article

Combined effects of rat Schwann cells and 17β-estradiol in a spinal cord injury model

verfasst von: Zeinab Namjoo, Fateme Moradi, Roya Aryanpour, Abbas Piryaei, Mohammad Taghi Joghataei, Yusef Abbasi, Amir Hosseini, Sajad Hassanzadeh, Fatemeh Ranjbar Taklimie, Cordian Beyer, Adib Zendedel

Erschienen in: Metabolic Brain Disease | Ausgabe 4/2018

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Abstract

Spinal cord injury (SCI) is a devastating traumatic event which burdens the affected individuals and the health system. Schwann cell (SC) transplantation is a promising repair strategy after SCI. However, a large number of SCs do not survive following transplantation. Previous studies demonstrated that 17β-estradiol (E2) protects different cell types and reduces tissue damage in SCI experimental animal model. In the current study, we evaluated the protective potential of E2 on SCs in vitro and investigated whether the combination of hormonal and SC therapeutic strategy has a better effect on the outcome after SCI. Primary SC cultures were incubated with E2 for 72 h. In a subsequent experiment, thoracic contusion SCI was induced in male rats followed by sustained administration of E2 or vehicle. Eight days after SCI, DiI-labeled SCs were transplanted into the injury epicenter in vehicle and E2-treated animals. The combinatory regimen decreased neurological and behavioral deficits and protected neurons and oligodendrocytes in comparison to vehicle rats. Moreover, E2 and SC significantly decreased the number of Iba-1+ (microglia) and GFAP⁺ cells (astrocyte) in the SCI group. In addition, we found a significant reduction of mitochondrial fission-markers (Fis1) and an increase of fusion-markers (Mfn1 and Mfn2) in the injured spinal cord after E2 and SC treatment. These data demonstrated that E2 protects SCs against hypoxia-induced SCI and improves the survival of transplanted SCs.

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Titel: Combined effects of rat Schwann cells and 17β-estradiol in a spinal cord injury model
verfasst von: Zeinab Namjoo
Fateme Moradi
Roya Aryanpour
Abbas Piryaei
Mohammad Taghi Joghataei
Yusef Abbasi
Amir Hosseini
Sajad Hassanzadeh
Fatemeh Ranjbar Taklimie
Cordian Beyer
Adib Zendedel
Publikationsdatum: 15.04.2018
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 4/2018
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-018-0220-8

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Combined effects of rat Schwann cells and 17β-estradiol in a spinal cord injury model

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