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25.02.2020 | Original Article

Common and unique mechanisms of filamentous actin formation by viruses of the genus Orthorubulavirus

verfasst von: Keisuke Ohta, Yusuke Matsumoto, Machiko Nishio

Erschienen in: Archives of Virology | Ausgabe 4/2020

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Abstract

We previously found that infection with human parainfluenza virus type 2 (hPIV-2), a member of the genus Orthorubulavirus, family Paramyxoviridae, causes filamentous actin (F-actin) formation to promote viral growth. In the present study, we investigated whether similar regulation of F-actin formation is observed in infections with other rubulaviruses, such as parainfluenza virus type 5 (PIV-5) and simian virus 41 (SV41). Infection with these viruses caused F-actin formation and RhoA activation, which promoted viral growth. These results indicate that RhoA-induced F-actin formation is important for efficient growth of these rubulaviruses. Only SV41 and hPIV-2 V and P proteins bound to Graf1, while the V and P proteins of PIV-5, mumps virus, and hPIV-4 did not bind to Graf1. In contrast, the V proteins of these rubulaviruses bound to both inactive RhoA and profilin 2. These results suggest that there are common and unique mechanisms involved in regulation of F-actin formation by members of the genus Orthorubulavirus.

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Titel: Common and unique mechanisms of filamentous actin formation by viruses of the genus Orthorubulavirus
verfasst von: Keisuke Ohta
Yusuke Matsumoto
Machiko Nishio
Publikationsdatum: 25.02.2020
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2020
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04565-y

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Common and unique mechanisms of filamentous actin formation by viruses of the genus Orthorubulavirus

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