To determine the genotypic characteristics of the KP isolate (SB4936), a genomic sequence was obtained using an Illumina 2 x 300 nt paired end protocol on a MiSeq instrument. Reads were assembled de-novo using CLCbio assembler. The genomic sequence was submitted to the European Nucleotide Archive (accession number PRJEB9692). Contigs were scanned using the BIGSdb tool (
http://bigsdb.pasteur.fr) for core genome multilocus sequence typing (cgMLST) as well as KP virulence and resistance genes [
8,
9]. Phylogenetic analysis of gene sequences showed that the strain belongs to KP sensu stricto [
10]. Consistent with this, the genome sequence harbored the marker Kp50233, previously shown to be specific for KP [
11]. Analysis of the 7-gene Multilocus sequence typing (MLST) sequences [
12] showed that the isolate belongs to sequence type (ST) 86, the archetype ST of clonal group (CG) 86, previously recovered from severe community-acquired infections [
9,
13]. Furthermore, comparisons of the 694 cgMLST genes revealed only 19 allelic mismatches when compared to reference strain SA1 of ST86, which demonstrates that isolate SB4936 belongs to CG86 [
9,
13]. Consistently, the genome also harbored genes
kpiA and
nikA2, which are typical for this clonal group [
11]. It also possessed the following virulence factors:
rmpA and
rmpA2 (regulator of mucoid phenotype, associated with the hypermucoviscous phenotype),
iroBCDN (coding for salmochelin),
iucABCDiutA (coding for the aerobactin siderophore cluster),
kvgAS (a two-component system),
mrkABCDFHIJ (coding for the cluster for type III fimbriae involved in adhesion and biofilm formation). Conversely the
kfuABC (iron aquisition) and
allABCDRS (allantoin utilization) clusters were absent from its genome. The strain also possessed gene sequences
wzi-2 and
wzc-2, previously shown to be specifically encountered in strain of capsular serotype K2, another important virulence factor of KP. The strain did not harbor the
irp1 and
irp2 genes coding for polyketide synthase/non ribosomal peptide synthetase associated with yersinibactin siderophore synthesis. No resistance gene other than
blaSHV-1 was detected in the genome of SB4936 which was consistent with the antimicrobial susceptibility profile (resistance to only ampicillin, ticarcillin and piperacillin). The genomic features of isolate SB4936 were consistent with a hypervirulent strain with a wild-type susceptibility profile, as previously described for isolates of clonal group CG86 [
8,
9,
11,
14]. The strain also harbored the heavy metal resistance gene clusters
pbr (lead),
pco (copper),
sil (silver) and
ter (tellurium). The presence of these clusters as well as of
rmpA and the aerobactin cluster demonstrates the presence in SB4936 of a plasmid similar to pLVPK, the large virulence plasmid of
K. pneumoniae [
5]. Overall, 249 out of 251 protein-coding genes of pLVPK were present in SB4936, among which 237 were 100% identical in nucleotide sequence.