Background
Methods
Ethics statement
Articular cartilage specimens
NFH | Control | |||
---|---|---|---|---|
Age (years) | Sex | Age (years) | Sex | |
Microarray | 42 | Male | 45 | Male |
41 | Male | 42 | Male | |
51 | Male | 53 | Male | |
47 | Female | 47 | Female | |
qRT-PCR | 42 | Male | 42 | Male |
42 | Male | 54 | Male | |
43 | Male | 57 | Male | |
47 | Male | 61 | Male | |
47 | Male | 64 | Male | |
48 | Female | 60 | Female | |
54 | Female | 61 | Female | |
57 | Female | 63 | Female |
RNA preparation
Microarray hybridization
Identification of differently expressed genes
Gene Ontology enrichment analysis
Quantitative real-time PCR
Immunohistochemical analysis
Results
Differently expressed genes in articular cartilage from patients with NFH
Gene | Genbank ID | Function | Ratio |
---|---|---|---|
COL5A1
| NM_000093 | Extracellular matrix | 17.32 ± 4.95 |
CRTAC1
| NM_018058 | Extracellular matrix | 22.67 ± 3.19 |
CRLF1
| NM_004750 | Cytokines | 62.28 ± 6.61 |
COL6A3
| NM_004369 | Extracellular matrix | 12.80 ± 3.25 |
COL3A1
| NM_000090 | Extracellular matrix | 13.56 ± 4.24 |
OGN
| NM_033014 | Extracellular matrix | 10.78 ± 3.63 |
MT1F
| NM_005949 | Metallothionein | 6.08 ± 0.78 |
ANGPTL4
| NM_139314 | Growth factor | 26.83 ± 2.65 |
IGFBP7
| NM_001553 | Growth factor | 22.89 ± 6.02 |
COL6A1
| NM_001848 | Extracellular matrix | 8.73 ± 2.91 |
CRIP1
| NM_001311 | Cell cycle | 7.79 ± 1.88 |
SPP1
| NM_001040058 | Extracellular matrix | 17.61 ± 4.10 |
ASPN
| NM_017680 | Extracellular matrix | 22.15 ± 5.28 |
MXRA7
| NM_001008529 | Extracellular matrix | 7.94 ± 2.21 |
NFIL3
| NM_005384 | Transcription | 11.05 ± 1.92 |
MINOS1-NBL1
| NM_001204088 | Miscellaneous | 5.76 ± 1.57 |
METRNL
| NM_001004431 | Miscellaneous | 11.77 ± 3.40 |
P4HA2
| NM_004199 | Extracellular matrix | 6.96 ± 1.47 |
COL1A1
| NM_000088 | Extracellular matrix | 51.81 ± 15.49 |
TSC22D3
| NM_004089 | Cytokines | 7.39 ± 1.92 |
ID2
| NM_002166 | Cell cycle | 20.62 ± 5.78 |
PRG4
| NM_005807 | Cytokines | 13.87 ± 3.72 |
CD55
| NM_000574 | Apoptosis | 6.53 ± 1.52 |
STEAP1
| NM_012449 | Transmembrane protein | 7.31 ± 2.21 |
TMEM171
| NM_173490 | Miscellaneous | 0.34 ± 0.05 |
MDK
| NM_001012334 | Cytokines | 0.47 ± 0.02 |
VKORC1
| AK125618 | Miscellaneous | 0.44 ± 0.09 |
GO enrichment analysis
GO term | GO ID | FESa
| FDR |
---|---|---|---|
Proteinaceous extracellular matrix | GO:0005578 | 15.63 | 3.22 × 10-5
|
Extracellular matrix | GO:0031012 | 14.50 | 5.78 × 10-5
|
Extracellular region part | GO:0044421 | 6.95 | 1.28 × 10-4
|
Collagen | GO:0005581 | 79.39 | 3.22 × 10-4
|
Extracellular region | GO:0005576 | 4.15 | 4.78 × 10-4
|
Platelet-derived growth factor binding | GO:0048407 | 224.81 | 5.23 × 10-4
|