Background
Methods
Research design and study population
Sample size and sample determination
Inclusion and exclusion criteria
Data collection
Anthropometric measurements
Biochemical analysis
Assessment of blood pressure
Assessment of PA
Statistical analysis
Results
Variables | Groups (n = 141) | P valueg | |||||
---|---|---|---|---|---|---|---|
Vit D (n = 24)a | Vit D + PA (n = 21)b | Vit C (n = 26)c | Vit C + PA (n = 23)d | Placebo (n = 25)e | Placebo + PA (n = 22)f | ||
Age (years) | 40.5 ± 5.9 | 40.4 ± 5.9 | 41.2 ± 5.8 | 40.8 ± 5.8 | 42.6 ± 5.6 | 41.6 ± 6.4 | .812 |
Female (%) | 67 | 67 | 62 | 70 | 48 | 59 | .725 |
Family history of obesity (%) | 75 | 81 | 65 | 65 | 76 | 50 | .314 |
Family history of DM (%) | 50 | 67 | 46 | 35 | 60 | 41 | .321 |
Family history of BP (%) | 58 | 62 | 46 | 57 | 60 | 41 | .753 |
Weight (kg) | 84.2 ± 16.7 | 81.7 ± 9.6 | 81 ± 13.2 | 79.7 ± 13.6 | 82.3 ± 14 | 74.9 ± 12.7 | .354 |
BMI (kg/m2) | 33.1 ± 5.9 | 33.4 ± 4.3 | 32 ± 6.1 | 32.3 ± 5.9 | 32.8 ± 4.3 | 30.1 ± 4.7 | .475 |
WC (cm) | 109.2 ± 8 | 108.5 ± 7.7 | 108.2 ± 10.4 | 107.8 ± 8.7 | 107.6 ± 9.6 | 111.5 ± 10.2 | .824 |
FPG (mg/dL) | 108 ± 17.1 | 106 ± 11.8 | 106.6 ± 19.9 | 104 ± 13.7 | 110.6 ± 17.2 | 106.2 ± 18.1 | .871 |
SBP (mmHg) | 127.1 ± 11.5 | 129 ± 12.7 | 130.2 ± 12 | 129 ± 11.4 | 125.6 ± 14.5 | 128.6 ± 10.8 | .810 |
DBP (mmHg) | 79.8 ± 9.5 | 83.6 ± 9.4 | 81.3 ± 8.3 | 79.3 ± 7.1 | 80 ± 6.9 | 82.5 ± 5.9 | .421 |
TC (mg/dL) | 173.5 ± 60.8 | 194.7 ± 32.2 | 174.8 ± 41.5 | 178.3 ± 29.8 | 185.9 ± 39 | 149 ± 35.8 | .326 |
TG (mg/dL) | 229.3 ± 113.8 | 184.5 ± 98.5 | 268.4 ± 107.2* | 176.1 ± 87.4 | 147.4 ± 43 | 161.7 ± 70.1 | .001 |
LDL-C (mg/dL) | 120.7 ± 64.4† | 149.6 ± 35.8 | 114.5 ± 47.5 | 135.5 ± 32.7 | 150.4 ± 39.8 | 153.5 ± 38.4 | .007 |
HDL-C (mg/dL) | 34.9 ± 17.3 | 40.9 ± 14.4 | 33 ± 13.5 | 37.6 ± 11** | 30 ± 8.5 | 40.8 ± 16.5 | .046 |
Vit D (ng/mL) | 10.8 ± 2.8 | 10.4 ± 3.2 | – | – | 12.2 ± 4 | 11 ± 4 | .434 |
Vit C (ng/mL) | – | – | .9 ± .4 | .8 ± .3 | 1 ± .3 | .9 ± .3 | .320 |
Variables | Groups (n = 141) | P valueg | |||||
---|---|---|---|---|---|---|---|
Vit D (n = 24)a | Vita D + PA (n = 21)b | Vit C (n = 26)c | Vit C + PA (n = 23)d | Placebo (n = 25)e | Placebo + PA (n = 22)f | ||
Weight (kg) | 84.9 ± 16.7 | 79 ± 11 | 79.5 ± 12.9 | 77.9 ± 13.4 | 82.3 ± 13.8 | 73.6 ± 12.5 | .124 |
BMI (kg/m2) | 33.3 ± 6 | 32.2 ± 4 | 311.4 ± 6 | 31.7 ± 5.6 | 32.8 ± 4.2 | 29.7 ± 4.7 | .231 |
WC (cm) | 109.1 ± 8.2 | 107.2 ± 7.5 | 107 ± 9.9 | 103.6 ± 8.4 | 106.2 ± 9.7 | 107.9 ± 10.4 | .442 |
FPG (mg/dL) | 97.8 ± 7.7 | 97.7 ± 8.7 | 105 ± 13.3 | 100.7 ± 8.4 | 108 ± 19.2 | 98.9 ± 10.7 | .012 |
SBP (mmHg) | 121.3 ± 9.2 | 126.2 ± 8.9 | 129.2 ± 8.6 | 173 ± 252.4 | 126.6 ± 12 | 123 ± 12 | .514 |
DBP (mmHg) | 80.1 ± 5.8 | 81.7 ± 5.3 | 81.9 ± 5 | 79.6 ± 4.5 | 81.6 ± 6.7 | 80.2 ± 6.7 | .643 |
TC (mg/dL) | 160.5 ± 33.4 | 81.7 ± 31.3 | 180.2 ± 31.9 | 175.1 ± 29.9 | 196.8 ± 39.4 | 184.8 ± 28.1 | .009 |
TG (mg/dL) | 233.8 ± 97 | 178 ± 80.8 | 246.1 ± 90.3 | 151.2 ± 53.8 | 158.6 ± 35.4 | 155.6 ± 57.7 | .001 |
LDL-C (mg/dL) | 107 ± 36.6 | 138.3 ± 31.4 | 124.3 ± 39.6 | 134.5 ± 28.2 | 158.8 ± 39 | 145.8 ± 30.2 | .001 |
HDL-C (mg/dL) | 33.7 ± 10.6 | 39.1 ± 10 | 33.2 ± 10.1 | 51.9 ± 20.6 | 31.8 ± 7.1 | 38.9 ± 9.8 | .001 |
Vit D (ng/mL) | 23.2 ± 4.9 | 29 ± 5.5 | – | – | 12.6 ± 4 | 18.9 ± 4.5 | .001 |
Vit C (ng/mL) | – | – | 1.4 ± .3 | 1.7 ± .3 | .9 ± .4 | 1.1 ± .3 | .001 |
Variables | Groups (n = 141) | P valueg | |||||
---|---|---|---|---|---|---|---|
Vit D (n = 24)a | Vita D + PA (n = 21)b | Vit C (n = 26)c | Vit C + PA (n = 23)d | Placebo (n = 25)e | Placebo + PA (n = 22)f | ||
Weight (kg) | .4 ± 1.3 | − 2.8 ± 7.9 | − 1.6 ± 2.2 | − 1.8 ± 4.3 | − .03 ± 1.3 | − 1.2 ± 1 | .058 |
BMI (kg/m2) | .16 ± .5 | − 1.3 ± 3.5 | − .6 ± .8 | − .6 ± 1.3 | − .01 ± .5 | − .5 ± .4 | .031 |
WC (cm) | − .04 ± 2.1 | − 1.2 ± 2.2 | − 1.2 ± 2.3 | − 4.2 ± 6.1 | − 1.4 ± 3.6 | − 3.6 ± 1.4 | .001 |
FPG (mg/dL) | − 10.2 ± 15.9 | − 8.3 ± 16.2 | − 1.7 ± 11 | − 3.3 ± 14.3 | − 2.6 ± 19.7 | − 7.4 ± 14.6 | .325 |
SBP (mmHg) | − 5.8 ± 7.1 | − 2.9 ± 9.7 | − .1 ± 8 | 44.1 ± 251.1 | 1 ± 8.2 | − 5.7 ± 7.9 | .552 |
DBP (mmHg) | 1 ± 6.9 | − 1.9 ± 7.7 | .6 ± 7.4 | .2 ± 8.2 | 1.6 ± 8.7 | − 2.3 ± 6.3 | .434 |
TC (mg/dL) | − 13 ± 42.3 | − 13 ± 16.7 | 5.4 ± 24.9 | − 3.1 ± 22.4 | 10.9 ± 32.8 | − 9.3 ± 17 | .012 |
TG (mg/dL) | 4.6 ± 32.9 | − 6.4 ± 29.4 | − 22.3 ± 31.2 | − 25 ± 68.8 | 11.2 ± 29.8 | − 6.1 ± 18.8 | .006 |
LDL-C (mg/dL) | − 13.7 ± 43.8 | − 11.3 ± 19.8 | 9.8 ± 24.8 | − 1 ± 27.8 | 8.3 ± 30.9 | − 7.7 ± 17.9 | .017 |
HDL-C (mg/dL) | − 1.3 ± 12.9 | − 1.9 ± 13.4 | .1 ± 11.1 | 14.3 ± 20 | 1.7 ± 7.2 | − 1.9 ± 13.9 | .001 |
Vit D (ng/mL) | 12.4 ± 4.3 | 18.6 ± 6.8 | – | – | .5 ± 1.3 | 7.9 ± 2.8 | .001 |
Vit C (ng/mL) | – | – | .5 ± .5 | .9 ± .4 | − .07 ± .5 | .1 ± .5 | .001 |
Multiple comparisons: Tukey HSD | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Dependent variables | Mean difference (I–J) | Std. error | Sig. | 95% confidence interval | Mean difference (I–J) | Std. error | Sig. | 95% confidence interval | |||
(I) Group | (J) Group | Lower bound | Upper bound | Lower bound | Upper bound | ||||||
Changes in BMI | Change in WC | ||||||||||
Vit Da | Vit D + PA | 1.4* | .45 | .029 | .09 | 2.72 | 1.19 | .99 | .831 | − 1.6 | 4.07 |
Vit C | .74 | .43 | .512 | − .50 | 1.99 | 1.18 | .94 | .802 | − 1.5 | 3.91 | |
Vit C + PA | .79 | .44 | .473 | − .48 | 2.08 | 4.17* | .97 | .001 | 1.3 | 6.98 | |
Placebo | .16 | .43 | .991 | − 1.09 | 1.42 | 1.35 | .95 | .714 | − 1.3 | 4.11 | |
Placebo + PAf | .63 | .44 | .722 | − .66 | 1.93 | 3.54* | .98 | .006 | .70 | 6.39 | |
Vit Cc | Vit D + PA | .66 | .44 | .675 | − .63 | 1.95 | .007 | .97 | 1.01 | − 2.8 | 2.83 |
Vit C + PA | .05 | .43 | 1.04 | − 1.20 | 1.31 | 2.98* | .95 | .021 | .22 | 5.74 | |
Placebo | − .57 | .42 | .755 | − 1.81 | .65 | .16 | .93 | 1.03 | − 2.52 | 2.86 | |
Placebo + PA | − .11 | .44 | 1.01 | − 1.38 | 1.16 | 2.36 | .96 | .142 | − .43 | 5.15 | |
Vit D + PAb | Vit C + PA | − .60 | .45 | .772 | − 1.93 | .72 | 2.97* | 1.00 | .041 | .07 | 5.88 |
Placebo | − 1.23 | .45 | .074 | − 2.54 | .06 | .16 | .98 | 1.01 | − 2.68 | 3.01 | |
Placebo + PA | − .77 | .46 | .551 | − 2.11 | .56 | 2.35 | 1.01 | .192 | − .58 | 5.29 | |
Vit C + PAd | Placebo | − .63 | .43 | .705 | − 1.90 | .64 | − 2.81* | .96 | .041 | − 5.60 | − .034 |
Placebo + PA | − .16 | .45 | .998 | − 1.47 | 1.14 | − .62 | .99 | .987 | − 3.49 | 2.24 | |
Placeboe | Placebo + PA | .46 | .44 | .901 | − .82 | 1.75 | 2.19 | .97 | .221 | − .62 | 5.00 |
Change in TC | Change in TG | ||||||||||
Vit Da | Vit D + PA | − .047 | 8.33 | 1.00 | − 24.15 | 24.05 | 10.96 | 11.48 | .931 | − 22.23 | 44.16 |
Vit C | − 18.38 | 7.89 | .190 | − 41.21 | 4.44 | 26.86 | 10.87 | .141 | − 4.59 | 58.31 | |
Vit C + PA | − 9.86 | 8.14 | .830 | − 33.40 | 13.66 | 29.52 | 11.21 | .096 | − 2.89 | 61.95 | |
Placebo | − 23.92* | 7.97 | .037 | − 46.97 | − .86 | − 6.64 | 10.98 | .990 | − 38.40 | 25.10 | |
Placebo + PAf | − 3.72 | 8.23 | .998 | − 27.53 | 20.08 | 10.67 | 11.34 | .935 | − 22.12 | 43.47 | |
Vit Cc | Vit D + PA | 18.33 | 8.18 | .227 | − 5.33 | 42.00 | − 15.89 | 11.27 | .721 | − 48.49 | 16.70 |
Vit C + PA | 8.51 | 7.98 | .894 | − 14.57 | 31.60 | 2.66 | 11.00 | 1.00 | − 29.14 | 34.47 | |
Placebo | − 5.53 | 7.81 | .981 | − 28.13 | 17.059 | − 33.51* | 10.76 | .027 | − 64.63 | − 2.38 | |
Placebo + PA | 14.65 | 8.08 | .461 | − 8.71 | 38.02 | − 16.18 | 11.13 | .694 | − 48.37 | 16.00 | |
Vit D + PAb | Vit C + PA | − 9.82 | 8.42 | .852 | − 34.16 | 14.52 | 18.56 | 11.60 | .600 | − 14.97 | 52.09 |
Placebo | − 23.8* | 8.25 | .050 | − 47.74 | .005 | − 17.61 | 11.37 | .634 | − 50.50 | 15.27 | |
Placebo + PA | − 3.67 | 8.51 | .998 | − 28.28 | 20.92 | − .29 | 11.72 | 1.00 | − 34.19 | 33.60 | |
Vit C + PAd | Placebo | − 14.05 | 8.06 | .506 | − 37.35 | 9.25 | − 36.17* | 11.10 | .017 | − 68.28 | − 4.07 |
Placebo + PA | 6.14 | 8.32 | .977 | − 17.91 | 30.19 | − 18.85 | 11.46 | .571 | − 51.98 | 14.28 | |
Placeboe | Placebo + PA | 20.19 | 8.15 | .139 | − 3.38 | 43.77 | 17.32 | 11.23 | .638 | − 15.15 | 49.80 |
Change in LDL-C | Change in HDL-C | ||||||||||
Vit Da | Vit D + PA | − 2.36 | 8.68 | 1.00 | − 27.46 | 22.74 | .60 | 4.03 | 1.00 | − 11.07 | 12.27 |
Vit C | − 23.47* | 8.22 | .055 | − 47.25 | .30 | − 1.39 | 3.82 | .992 | − 12.45 | 9.66 | |
Vit C + PA | − 12.65 | 8.47 | .669 | − 37.17 | 11.85 | − 15.57* | 3.94 | .002 | − 26.97 | − 4.17 | |
Placebo | − 21.99 | 8.30 | .093 | − 46.00 | 2.01 | − 2.96 | 3.86 | .972 | − 14.13 | 8.19 | |
Placebo + PAf | − 5.99 | 8.57 | .982 | − 30.79 | 18.79 | .68 | 3.98 | 1.00 | − 10.84 | 12.22 | |
Vit Cc | Vit D + PA | 21.11 | 8.52 | .138 | − 3.53 | 45.76 | 2.0 | 3.96 | .994 | − 9.46 | 13.46 |
Vit C + PA | 10.81 | 8.31 | .784 | − 13.23 | 34.86 | − 14.18* | 3.86 | .005 | − 25.36 | − 2.99 | |
Placebo | 1.481 | 8.13 | 1.00 | − 22.05 | 25.01 | − 1.57 | 3.78 | .998 | − 12.51 | 9.37 | |
Placebo + PA | 17.47 | 8.41 | .306 | − 6.85 | 41.81 | 2.08 | 3.91 | .995 | − 9.23 | 13.40 | |
Vit D + PAb | Vit C + PA | − 10.29 | 8.77 | .848 | − 35.65 | 15.05 | − 16.18* | 4.07 | .002 | − 27.97 | − 4.38 |
Placebo | − 19.63 | 8.60 | .208 | − 44.50 | 5.23 | − 3.57 | 4.00 | .941 | − 15.13 | 7.99 | |
Placebo + PA | − 3.63 | 8.86 | .998 | − 29.26 | 21.99 | .083 | 4.12 | 1.00 | − 11.83 | 12.00 | |
Vit C + PAd | Placebo | − 9.33 | 8.39 | .876 | − 33.61 | 14.93 | 12.60* | 3.90 | .012 | 1.31 | 23.89 |
Placebo + PA | 6.65 | 8.66 | .972 | − 18.39 | 31.71 | 16.26* | 4.03 | .001 | 4.61 | 27.91 | |
Placeboe | Placebo + PA | 15.99 | 8.49 | .417 | − 8.56 | 40.55 | 3.65 | 3.95 | .931 | − 7.76 | 15.07 |