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Medical Microbiology and Immunology

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01.10.2016 | Original Investigation

Comparative evaluation of two Rickettsia typhi-specific quantitative real-time PCRs for research and diagnostic purposes

verfasst von: Stefanie Papp, Jessica Rauch, Svenja Kuehl, Ulricke Richardt, Christian Keller, Anke Osterloh

Erschienen in: Medical Microbiology and Immunology | Ausgabe 1/2017

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Abstract

Rickettsioses are caused by intracellular bacteria of the family of Rickettsiaceae. Rickettsia (R.) typhi is the causative agent of endemic typhus. The disease occurs worldwide and is one of the most prevalent rickettsioses. Rickettsial diseases, however, are generally underdiagnosed which is mainly due to the lack of sensitive and specific methods. In addition, methods for quantitative detection of the bacteria for research purposes are rare. We established two qPCRs for the detection of R. typhi by amplification of the outer membrane protein B (ompB) and parvulin-type PPIase (prsA) genes. Both qPCRs are specific and exclusively recognize R. typhi but no other rickettsiae including the closest relative, R. prowazekii. The prsA-based qPCR revealed to be much more sensitive than the amplification of ompB and provided highly reproducible results in the detection of R. typhi in organs of infected mice. Furthermore, as a nested PCR the prsA qPCR was applicable for the detection of R. typhi in human blood samples. Collectively, the prsA-based qPCR represents a reliable method for the quantitative detection of R. typhi for research purposes and is a promising candidate for differential diagnosis.

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Titel: Comparative evaluation of two Rickettsia typhi-specific quantitative real-time PCRs for research and diagnostic purposes
verfasst von: Stefanie Papp
Jessica Rauch
Svenja Kuehl
Ulricke Richardt
Christian Keller
Anke Osterloh
Publikationsdatum: 01.10.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Medical Microbiology and Immunology / Ausgabe 1/2017
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI: https://doi.org/10.1007/s00430-016-0480-z

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Comparative evaluation of two Rickettsia typhi-specific quantitative real-time PCRs for research and diagnostic purposes

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