Comparative in vitro anti-tick efficacy of commercially available products and newly developed phyto-formulations against field collected and resistant tick lines of Rhipicephalus (Boophilus) microplus

Rhipicephalus (Boophilus) microplus is considered as one of the most widely distributed tick species ecto-parasitizing on livestock and causes fatal diseases with significant production loss. To address the problem of controlling acaricide resistant tick infestations on animals, attention has been paid to develop eco-friendly phyto-acaricides. The present study was undertaken to evaluate the comparative anti-tick activities of commercially available herbal and chemical products with chemically characterized phyto-formulations developed recently against field ticks and resistant tick lines of R. (B.) microplus. The chemical product Butox^® Vet was found nearly passive against all the tested resistant tick lines. However, one of the commercial polyherbal product, Zerokeet^® showed an efficacy (E%) of 41.8–75.4 % (\(\bar{X} = 63.5 \pm 7.4\,\%\)) using recommended dilution (1:2) against field ticks and resistant tick lines. However, the other commercial product, Erina^® EP has very limited efficacy against all the tested tick. In comparison, the newly developed phyto-formulation, NBA/13/B/2 and NAC-01 conferred an E% of 82.4–91.3 % (\(\bar{X} = 85.9 \pm 2.1\,\%\)) and 62.3–94.6 % (\(\bar{X} = 80.0 \pm 8.1\,\%\)), respectively, against tested resistant ticks. Results indicated higher marketing potentiality of newly developed formulation in the existing tick problem scenario.