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Journal of Natural Medicines
Erschienen in:

07.05.2018 | Original Paper

Comparative transcriptome analyses of three medicinal Forsythia species and prediction of candidate genes involved in secondary metabolisms

verfasst von: Luchao Sun, Amit Rai, Megha Rai, Michimi Nakamura, Noriaki Kawano, Kayo Yoshimatsu, Hideyuki Suzuki, Nobuo Kawahara, Kazuki Saito, Mami Yamazaki

Erschienen in: Journal of Natural Medicines | Ausgabe 4/2018

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Abstract

The three Forsythia species, F. suspensa, F. viridissima and F. koreana, have been used as herbal medicines in China, Japan and Korea for centuries and they are known to be rich sources of numerous pharmaceutical metabolites, forsythin, forsythoside A, arctigenin, rutin and other phenolic compounds. In this study, de novo transcriptome sequencing and assembly was performed on these species. Using leaf and flower tissues of F. suspensa, F. viridissima and F. koreana, 1.28–2.45-Gbp sequences of Illumina based pair-end reads were obtained and assembled into 81,913, 88,491 and 69,458 unigenes, respectively. Classification of the annotated unigenes in gene ontology terms and KEGG pathways was used to compare the transcriptome of three Forsythia species. The expression analysis of orthologous genes across all three species showed the expression in leaf tissues being highly correlated. The candidate genes presumably involved in the biosynthetic pathway of lignans and phenylethanoid glycosides were screened as co-expressed genes. They express highly in the leaves of F. viridissima and F. koreana. Furthermore, the three unigenes annotated as acyltransferase were predicted to be associated with the biosynthesis of acteoside and forsythoside A from the expression pattern and phylogenetic analysis. This study is the first report on comparative transcriptome analyses of medicinally important Forsythia genus and will serve as an important resource to facilitate further studies on biosynthesis and regulation of therapeutic compounds in Forsythia species.
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Metadaten
Titel
Comparative transcriptome analyses of three medicinal Forsythia species and prediction of candidate genes involved in secondary metabolisms
verfasst von
Luchao Sun
Amit Rai
Megha Rai
Michimi Nakamura
Noriaki Kawano
Kayo Yoshimatsu
Hideyuki Suzuki
Nobuo Kawahara
Kazuki Saito
Mami Yamazaki
Publikationsdatum
07.05.2018
Verlag
Springer Japan
Erschienen in
Journal of Natural Medicines / Ausgabe 4/2018
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-018-1218-6

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