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27.09.2018 | Original Paper

Comparing protein and mRNA expressions of the human epidermal growth factor receptor family in estrogen receptor-positive breast cancer

verfasst von: Sasagu Kurozumi, Yuri Yamaguchi, Hiroshi Matsumoto, Kenichi Inoue, Masafumi Kurosumi, Tetsunari Oyama, Jun Horiguchi, Takaaki Fujii, Ken Shirabe

Erschienen in: Medical Molecular Morphology | Ausgabe 2/2019

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Abstract

The human epidermal growth factor receptor (HER) family plays a vital role in the development of resistance to treatments in estrogen receptor (ER)-positive breast cancer. This study investigated the correlation between protein and mRNA expressions of the HER family in ER-positive breast cancer. We dissected regions of invasive cancer from the frozen tissues of 34 patients with ER-positive breast cancer using laser-capture microdissection, followed by evaluation of the mRNA levels of the ER and HER family (EGFR, HER2, HER3, and HER4) using the quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. In addition, we assessed the protein expressions of the ER and HER family using an immunohistochemical (IHC) assay. A significant correlation was observed between the ER protein and mRNA expressions. For HER2, HER3, and HER4, protein expressions significantly correlated with mRNA levels. We established significant correlations of the mRNA level between EGFR versus HER2, as well as EGFR versus HER3. Furthermore, a significant correlation of the mRNA level between HER2 and HER3 was illustrated. In conclusion, IHC evaluation may be reliable and representable for mRNA. Hence, this study established a marked correlation between the mRNA expressions of HER family members in patients with ER-positive breast cancer.

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Titel: Comparing protein and mRNA expressions of the human epidermal growth factor receptor family in estrogen receptor-positive breast cancer
verfasst von: Sasagu Kurozumi
Yuri Yamaguchi
Hiroshi Matsumoto
Kenichi Inoue
Masafumi Kurosumi
Tetsunari Oyama
Jun Horiguchi
Takaaki Fujii
Ken Shirabe
Publikationsdatum: 27.09.2018
Verlag: Springer Japan
Erschienen in: Medical Molecular Morphology / Ausgabe 2/2019
Print ISSN: 1860-1480
Elektronische ISSN: 1860-1499
DOI: https://doi.org/10.1007/s00795-018-0206-y

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Neu im Fachgebiet Pathologie

Theoretische Grundlagen von Explosionen und Explosionsverletzungen

Auswirkungen vorgeschalteter Laborprozesse auf die Digitalisierung histologischer Schnittpräparate

Knochenmarkhistologie bei Zytopenien

Herausforderungen der Automation bei der quantitativen Auswertung von Leberbiopsien