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Erschienen in: Journal of Parasitic Diseases 4/2016

23.06.2015 | Original Article

Comparison between in-house indirect ELISA and Dot-ELISA for the diagnosis of Fasciola gigantica in cattle

verfasst von: Jafar Arjmand Yamchi, Nasser Hajipour, Seyyed Meysam Abtahi Froushani, Mojtaba Keighobadi

Erschienen in: Journal of Parasitic Diseases | Ausgabe 4/2016

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Abstract

This survey was done to investigate the efficacy of the in-house indirect ELISA (iELISA) and Dot-ELISA methods Prepared from excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of Fasciola for sero-diagnosis of Fasciola gigantica in cattle. The liver specimens of slaughtered cattle were collected and their liver examined macroscopically and microscopically for infestation to fasciolosis. Sera from two groups of cattle, one infected with fasciolosis (n = 60) and the other non-infected with fasciolosis (n = 60), were used in the iELISA and Dot-ELISA test; grouping based on histopathology results. Except specificity, other parameters such as, sensitivity, accuracy, positive and negative predictive values of both Dot-ELISA and iELISA done with ES Ag were better than those of tests performed with Cr Ag. Interestingly, the reliability of two methods was very good similar to one another.
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Metadaten
Titel
Comparison between in-house indirect ELISA and Dot-ELISA for the diagnosis of Fasciola gigantica in cattle
verfasst von
Jafar Arjmand Yamchi
Nasser Hajipour
Seyyed Meysam Abtahi Froushani
Mojtaba Keighobadi
Publikationsdatum
23.06.2015
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 4/2016
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-015-0699-4

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