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Journal of Parasitic Diseases

Erschienen in:

08.02.2020 | Original Article

Comparison of camel, dog and the laboratory animals’ sera with the fetal calf serum (FCS) for cultivation of Leishmania major

verfasst von: Frideh Esfandiari, Amin Derakhshanfar, Fatemeh Goudarzi, Gholamreza Hatam

Erschienen in: Journal of Parasitic Diseases | Ausgabe 2/2020

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Abstract

The best-known serum for Leishmania spp. cultivation is the fetal calf serum (FCS), which is very expensive with ethical concerns. This study was conducted to compare various laboratory (BALB/c mice, rat, rabbit, hamster and guinea pig) and non-laboratory (dog and camel) animals’ sera as a substitute for FCS in L. major culture. L. major, MRHO/IR/75/ER strain, was cultivated in RPMI-1640 medium enriched with different percentages of mentioned animal’s sera. Parasite growth was checked constantly. The rate of growth and survival of parasites were compared with a control medium enriched with FCS. As well, biochemical (albumin, globulin AST, ALT, ALP, Bil, BUN, Crea, Ca, P, Na, K, Fe, TIBC, Mg, zinc, Chol, HDL, LDL, TG, BS, uric acid, LDH, CPK) analysis of all sera was performed and compared with FCS. The most promastigote growth rate is considered in 10% BALB/c, guinea pig and hamster sera on the 6th day of cultivation. Also, on the 8th day, parasites showed viability in all animal sera. The promastigote growth in culture media enriched with the camel and the dog sera in comparison with laboratory animals was considered very low. Differences between 10% FCS and 10% cocktail serum were not significant (p > 0.05) but with other sera were significant (p < 0.05). Also, differences between BALB/c with hamster and guinea pig sera were not significant, respectively (p = 0.07 and p = 0.09). According to the biochemical analysis of all sera, the higher content of iron was detected in the hamster, guinea pig, BALB/c and fetal calf sera. The magnesium and zinc content of guinea pig and BALB/c serum was found to be more than the others and comparable with FCS. The promastigote growth decreased by camel, dog and rat sera orderly. In this study, a rapid increase in parasite growth in media supplemented with hamster, BALB/c and guinea pig sera was considered. It could be suggested to use these sera as a suitable alternative for FCS in molecular biology researches.

Bahrami S, Hatam GR, Razavi M, Nazifi S (2011) In vitro cultivation of axenic amastigotes and the comparison of antioxidant enzymes at different stages of Leishmania tropica. Trop Biomed 28(2):411–417

Bessat M, Shanat SE (2013) Leishmaniasis: epidemiology, control and future perspectives with special emphasis on Egypt. J Trop Dis 3(1):1–10

Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G (2010) Serum-free cell culture:the serum-free media interactive online database. Altex 27(1):53–62

Esfandiari F, Sarkaril B, Turki H, Arefkhah N, Shakouri N (2018) Level of circulating steroid hormones in malaria and cutaneous leishmaniasis: a case control study. J Parasit Dis 43(1):54–58PubMedCentral

Fakhar M, Mirzaii M, Rafiei A (2012) Comparison hydatid cyst fluid with fetal bovine serum in culture of rat fibroblast cells. J Mazandaran Univ Med Sci 21(86):213–221 [In Persian]

Ferreira KA, Lemos-Júnior PE, Lages-Silva E, Ramírez LE, Pedrosa AL (2007) Human urine stimulates in vitro growth of Trypanosoma cruzi and Trypanosoma rangeli. Parasitol Res 101(5):1383–1388

Filipic B, Shehata M, Toth S, Schwarzmeier J, Koren S (2002) Novel serum replacement based on bovine ocular fluid: a useful tool for cultivation of different animal cells in vitro. Altex 19(1):15–20

Goodarzi P, Arjmand B, Emami-Razavi SH, Soleimani M, Khodadadi A, Mohamadi-Jahani F, Aghayan HR (2014) Human autologous serum as a substitute for fetal bovine Serum in human schwann cell culture. Acta Medica Iranica 52(4):241–245

Gospodarowicz D, Charles III (1980) Extracellular matrix and control of proliferation of vascular endothelial cells. J Clin Invest 65(6):1351–1364PubMedCentral

Grekov I, Svobodova M, Nohynkova E, Lipoldova M (2011) Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium. J Microbiol Methods 87(3):273–277

Gstraunthaler G (2003) Alternatives to the use of fetal bovine serum: serum-free cell culture. Altex 20(4):275–281

Hatam GR, Ghatee MA, Hossini SMH, Sarkari B (2009) Improvement of the newly developed latex agglutination test (Katex) for diagnosis of visceral lieshmaniasis. J Clin Lab Anal 23(4):202–205PubMedCentral

Hayashi I, Sato GH (1976) Replacement of serum by hormones permits growth of cells in a defined medium. Nature 259:132–134

Herman R (1966) Studies of the numbers and morphology of the intracellular form of Leishmania donovani grown in cell culture. J Eukaryot Microbiol 13(3):408–418

Hill CS, Treisman R (1995) Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell 80(2):199–211

Limoncu ME, Ozbilgin A, Balcioglu IC, Ozbel Y (2004) Evaluation of three new culture media for the cultivation and isolation of Leishmania parasites. J Basic Microbiol 44(3):197–202

Muniaraj M, Lal CS, Kumar S, Sinha PK, Das P (2007) Milk of cow (Bos Taurus), Buffalo, (Bubalus bubalis) and Goat (Capra hircicus): a better alternative than fetal bovine serum in media for primary isolation, in vitro cultivation and maintenance of L. donovani promastigotes. J Clin Microbiol 45(4):1353–1560PubMedCentral

Nasiri A, Habibi Gh, Esmailnia K (2011) Use of chicken serum as a good replacement for the fetal calf serum in cultivation of promastigotes of Leishmania major. J Arch Razi Inst 66(1):59–64

Pucadyil TJ, Tewary P, Madhubala R, Chattopadhyay A (2004) Cholesterol is required for Leishmania donovani infection: implications in leishmaniasis. Mol Biochem Parasitol 133(2):145–152

Rashidi S, Kalantar K, Hatam G (2018) Using proteomics as a powerful tool to develop a vaccine against Mediterranean visceral leishmaniasis. J Parasit Dis 42(2):162–170PubMedCentral

Sasse M, Lengwinat T, Henklein P, Hlinak A, Schade R (2000) Replacement of fetal calf serum in cell cultures by an egg yolk factor with cholecystokinin/gastrin-like immunoreactivity. Altern Lab Anim 28(6):815–831

Schwartz KA, Lu G, Trosko JE, Chang CC (1984) Serum from outdated human platelet concentrates: an alternative supplement for tissue (fibroblast) culture media. Am J Hematol 17(1):23–27

Taub M (1990) The use of defined media in cell and tissue culture. Toxicol In Vitro 4(3):213–225

Torres MP, Ortega YR (2006) Neospora caninum antibodies in commercial fetal bovine serum. Vet Parasitol 140(3–4):352–355

van der Valk J, Mellor D, Brands R, Fischer R, Gruber F, Gstraunthaler G, Hellebrekers L, Hyllner J, Jonker FH, Prieto P, Thalen M, Baumans V (2004) The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol In Vitro 18(1):1–12

World Health Organization (1991) Information on the epidemiology and control of the leishmaniasis by country or territory World Health Organization (WHO) Geneva Switzerland. Leishmania 91:30

World Health Organization (2017) WHO Report on Global Surveillance of Epidemic-prone Infectious Diseases–Leishmaniasis

Titel: Comparison of camel, dog and the laboratory animals’ sera with the fetal calf serum (FCS) for cultivation of Leishmania major
verfasst von: Frideh Esfandiari
Amin Derakhshanfar
Fatemeh Goudarzi
Gholamreza Hatam
Publikationsdatum: 08.02.2020
Verlag: Springer India
Erschienen in: Journal of Parasitic Diseases / Ausgabe 2/2020
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-020-01203-x

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Comparison of camel, dog and the laboratory animals’ sera with the fetal calf serum (FCS) for cultivation of Leishmania major

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