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11.06.2019 | Technical Note - Tumor - Glioma

Comparison of commercial 5-aminolevulinic acid (Gliolan®) and the pharmacy-compounded solution fluorescence in glioblastoma

verfasst von: Santiago Garfias Arjona, Mónica Lara Almunia, Javier Ángel Ibáñez Domínguez, Olga Delgado Sánchez, Priam Villalonga, Ruth Villalonga-Planells, Javier Pierola Lopetegui, Joan Bestard Escalas, Albert Maimó Barceló, Marta Brell Doval

Erschienen in: Acta Neurochirurgica | Ausgabe 8/2019

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Abstract

Background

5-Aminolevulinic acid (5-ALA) has become an important assistant in glioblastoma (GB) surgery. Unfortunately, its price affects its widespread use.

Objective

The aim of this study was to compare commercial 5-ALA with the pharmacy-compounded solution.

Methods

Using first an in vitro experimental approach, different concentrations of the pharmacy-compounded solution and commercial 5-ALA were tested in U87MG, LN229, U373, and T98G commercial glioblastoma cell lines. Fluorescence intensity was compared for each concentration by flow cytometry. Mean fluorescence of culture supernatant and lysate samples were analyzed. In a second phase, both preparations were used for surgical glioblastoma resection and tumor samples were analyzed by confocal microscopy. Mean fluorescence intensity was analyzed for each preparation and compared.

Results

There was a high variability of fluorescence intensity between cell lines, but each cell line showed similar fluorescence for both preparations (compounded preparation and commercial 5-ALA). In the same way, both preparations had similar fluorescence intensity in glioblastoma samples.

Conclusion

Both, compounded and commercial 5-ALA preparations produce equivalent fluorescent responses in human glioblastoma cells. Fluorescence intensity is cell line specific, but fluorescent properties of both preparations are undistinguishable.

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Titel: Comparison of commercial 5-aminolevulinic acid (Gliolan®) and the pharmacy-compounded solution fluorescence in glioblastoma
verfasst von: Santiago Garfias Arjona
Mónica Lara Almunia
Javier Ángel Ibáñez Domínguez
Olga Delgado Sánchez
Priam Villalonga
Ruth Villalonga-Planells
Javier Pierola Lopetegui
Joan Bestard Escalas
Albert Maimó Barceló
Marta Brell Doval
Publikationsdatum: 11.06.2019
Verlag: Springer Vienna
Erschienen in: Acta Neurochirurgica / Ausgabe 8/2019
Print ISSN: 0001-6268
Elektronische ISSN: 0942-0940
DOI: https://doi.org/10.1007/s00701-019-03930-4

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Comparison of commercial 5-aminolevulinic acid (Gliolan®) and the pharmacy-compounded solution fluorescence in glioblastoma

Abstract

Background

Objective

Methods

Results

Conclusion

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Abstract

Background

Objective

Methods

Results

Conclusion

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