Background
Methods
Case
Chemicals
Components of Product-X
Preparation of diagnostic antigens, chemical-HSA adducts
Dot blotting
Measurements of immunological parameters
Results
Study participants
Dermatitis | ||
---|---|---|
Yes | No | |
Number of subjects | 11 | 9 |
Age | 43.5 ± 3.3 | 41.6 ± 3.9 |
Number of current smokers (%) | 8 (72.3 %) | 7 (77.8) |
Number of habitual drinkers (%) | 7 (63.6) | 6 (66.7) |
Allergic history (%) | 2 (18.2) | 4 (44.4) |
Specific IgE against inhalant and food antigens and CD4/CD8 ratio
Dermatitis | ||||||
---|---|---|---|---|---|---|
Yes | No | |||||
Units | Median | (Range) | Median | (Range) | ||
Nonspecific IgE | IU/mL | 84.3 | (15.1 - 442) | 76.6 | (6.9 – 1,930) | n.s. |
Dermatophagoides farinae
| lumicount | 0.44 | (0–68.3) | 20.8 | (0.24 - 99.9) | p < 0.05 |
House dust | lumicount | 0.32 | (0–3.43) | 0.38 | (0–19.1) | n.s. |
Cat | lumicount | 0.16 | (0–5.19) | 0.23 | (0–1.46) | n.s. |
Dog | lumicount | 0.28 | (0.07 - 0.56) | 0.43 | (0–24.1) | n.s. |
Timothy grass | lumicount | 0.32 | (0.16 - 18.4) | 0.8 | (0–99.6) | n.s. |
Vernal grass | lumicount | 0.09 | (0–12.7) | 0.33 | (0–75.3) | n.s. |
Ragweed mix | lumicount | 0.06 | (0–0.16) | 0.02 | (0–4.46) | n.s. |
Mugwort | lumicount | 0.08 | (0–1.93) | 0 | (0–96.4) | n.s. |
Japanese cedar | lumicount | 0.72 | (0–81.5) | 0.65 | (0–49.8) | n.s. |
Penicillin | lumicount | 0 | (0–0.07) | 0 | (0–2.38) | n.s. |
Cladosporium | lumicount | 0 | (0–0.25) | 0.01 | (0–2.08) | n.s. |
Candida | lumicount | 0 | (0–0.33) | 0 | (0–1.17) | n.s. |
Alternaria | lumicount | 0 | (0–0.12) | 0 | (0–0.77) | n.s. |
Aspergillus | lumicount | 0.13 | (0–0.32) | 0.3 | (0–3.99) | n.s. |
Wheat | lumicount | 0 | (0–0.1) | 0 | (0–1.56) | n.s. |
Soy bean | lumicount | 0.05 | (0–0.21) | 0.02 | (0–2.58) | n.s. |
Rice | lumicount | 0.02 | (0–0.14) | 0 | (0–2.93) | n.s. |
Tuna | lumicount | 0.02 | (0–0.26) | 0.11 | (0–1.26) | n.s. |
Salmon | lumicount | 0.05 | (0–0.23) | 0.09 | (0–0.59) | n.s. |
Shrimp | lumicount | 0 | (0–0.27) | 0.18 | (0–0.98) | p < 0.05 |
Crab | lumicount | 0.04 | (0–0.22) | 0.13 | (0–9.48) | n.s. |
Cheddar cheese | lumicount | 0.01 | (0–0.27) | 0 | (0–0.21) | n.s. |
Milk | lumicount | 0.08 | (0–0.42) | 0 | (0–1.13) | n.s. |
Beef | lumicount | 0 | (0–0.16) | 0 | (0–0.8) | n.s. |
Chicken | lumicount | 0 | (0–0.08) | 0.01 | (0–1.02) | n.s. |
Egg white | lumicount | 0.12 | (0–0.36) | 0.08 | (0–0.53) | n.s. |
Cortisol | ug/dL | 4.8 | (2.5 - 12) | 5 | (3.5 - 19.4) | n.s. |
IFN-gamma | IU/mL | Under detection limit | Under detection limit | |||
IL-1beta | pg/mL | 5 | (5–5) | 5 | (5–13) | n.s. |
IL-2 | U/mL | Under detection limit | Under detection limit | |||
IL-5 | pg/mL | 6.2 | (2.5 - 9) | 5.3 | (2.5 - 9) | n.s. |
IL-4 | pg/mL | 10.8 | (3.6 - 31.1) | 7.2 | (3.6 - 11.1) | n.s. |
IL-6 | pg/mL | 1.2 | (0.6 - 18.6) | 1.3 | (8–41.2) | n.s. |
CD8(+)CD4(+) | % | 1.5 | (0.4 - 5.5) | 0.8 | (0.4 - 1.9) | n.s. |
CD8(+)CD4(−) | % | 26.8 | (14.5 - 37.7) | 25.3 | (15.2 - 35.1) | n.s. |
CD8(−)CD4(+) | % | 49.3 | (31.8 - 57.8) | 47.7 | (43.7 - 62.2) | n.s. |
CD8(−)CD4(−) | % | 21.7 | (15.1 - 37.2) | 25.3 | (16.1 - 27.5) | n.s. |
CD4/CD8 ratio | 1.77 | (1.04 - 3.11) | 1.92 | (1.23 - 4.01) | n.s. |
Detection of IgG which reacts with chemical-HSA adduct
Prevalence rates of IgG and IgE which reacts with chemical-HSA adduct in the workers
Dermatitis | |||
---|---|---|---|
Yes (N = 11) | No (N = 9) | ||
IgE against BADGE | 0 (0.0 %) | 0 (0.0 %) | n.s. |
IgE against XDA | 0 (0.0) | 0 (0.0) | n.s. |
IgE against BEE | 0 (0.0) | 0 (0.0) | n.s. |
IgE against Product-X | 0 (0.0) | 0 (0.0) | n.s. |
IgG against BADGE | 3 (27.3) | 0 (0.0) | n.s. |
IgG against XDA | 1 (9.1) | 0 (0.0) | n.s. |
IgG against BEE | 1 (9.1) | 0 (0.0) | n.s. |
IgG against Product-X | 11 (100) | 5 (55.6) | p < 0.05 |