The online version of this article (doi:10.1186/1475-2875-11-210) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
PH and SH designed the study, analysed the data and prepared the manuscript. SH and KR carried out the experiments. All authors read and approved the final manuscript.
Several electroporation protocols exist to transfect exogenous DNA into Plasmodium falciparum. To date, however, only a subjective analysis of their relative efficiencies has been reported.
A time-course of luciferase reporter expression is used to provide an objective quantitative analysis of the absolute efficiency of three electroporation techniques; direct electroporation of ring stage infected erythrocytes, preloading of erythrocytes and a novel “double-tap” protocol that combines both approaches.
Preloading of erythrocytes shows a mean efficiency of 9.59x10-6, some 5–180 fold more efficient than matched experiments utilizing the “double-tap” and direct electroporation of ring stage infected erythrocytes alone, respectively.
Evidence presented here provides the first quantitative assessment of both the absolute and relative efficiencies of a key molecular tool used to study the biology and pathogenesis of this important human pathogen.
Authors’ original file for figure 112936_2012_2122_MOESM1_ESM.pdf
Balu B, Adamas JH: Advancements in transfection technologies for Plasmodium. Int J Parasitol. 2007, 26: 1- CrossRef
Carvalho TG, Ménard R: Manipulating the Plasmodium genome. Curr Issues Mol Biol. 2005, 7: 39- PubMed
Spalding MD, Allary M, Gallagher JR, Prigge ST: Validation of a modified method for Bxb1 mycobacteriophage integrase-mediated recombination in Plasmodium falciparum by localization of the H-protein of the glycine cleavage complex to the mitochondrion. Mol Biochem Parasitol. 2010, 172: 156-10.1016/j.molbiopara.2010.04.005. PubMedCentralCrossRefPubMed
- Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum
Karen T Russell
- BioMed Central
Neu im Fachgebiet Innere Medizin
Meistgelesene Bücher aus der Inneren Medizin
Mail Icon II