Comparison of three immunochromatographic assays for the rapid detection of CTX-M in Enterobacterales
- 04.11.2025
- Research
- Verfasst von
- Eugenie Maurin
- Emilie Mallecot
- Racha Beyrouthy
- Richard Bonnet
- Frederic Robin
Abstract
Purpose
Two lateral flow immunochromatographic assays are currently available for the rapid detection of CTX-M enzymes in Enterobacterales, RESIST CTX-M (Coris BioConcept, Gembloux, Belgium) and NG-Test CTX-M MULTI (NG Biotech, Guipry, France). The NG-Test CTX-M MULTI is designed to detect all CTX-M enzymes. The RESIST CTX-M detects CTX-M from the M-15 and the M-14 group for V1.0, CTX-M from the M-15 and the M-14/M-8 groups for V1.1.
Method
The performances of the two versions of the RESIST CTX-M test (V1.0 and V1.1) and the NG-Test CTX-M MULTI were assessed against a collection of 140 non-redundant Enterobacterales isolates characterized for their content in β-lactamases. β-lactamases content was established from genotypic analysis to collect a broad diversity of resistance mechanisms and bacterial strains, including 74 ESBL-producing strains (CTX-M (n = 56), other ESBLs (n = 18) either alone or associated with other β-lactamases), 22 strains overproducing chromosomal AmpC, 13 strains producing plasmid-encoded AmpC, 7 carbapenemase-producing strains, 3 strains combining the production of several β-lactamases other than ESBL and 21 strains that produced other β-lactamases.
Results
The sensitivity and specificity of NG-Test CTX-M MULTI for detecting CTX-M were 98.2% and 85.7%, respectively. They were 87.2% and 93.5% for RESIST CTX-M V1.0 for CTX-M of the M-1 and M-9 groups versus 94.3% and 93.1% for RESIST CTX-M V1.1 for CTX-M of the M-1 and M-9/8 groups.
Conclusion
Lateral flow immunochromatographic assays are rapid and reliable tests for the detection of CTX-M production. The NG-Test CTX-M MULTI and RESIST CTX-M V1.1 were the most efficient. However, one limitation of the different assays is the risk of false-positive results with strains belonging to the K. oxytoca and C. farmeri/sedlakii/amalonaticus complexes, which overproduce their chromosomal β-lactamase.
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- Titel
- Comparison of three immunochromatographic assays for the rapid detection of CTX-M in Enterobacterales
- Verfasst von
-
Eugenie Maurin
Emilie Mallecot
Racha Beyrouthy
Richard Bonnet
Frederic Robin
- Publikationsdatum
- 04.11.2025
- Verlag
- Springer Berlin Heidelberg
- Erschienen in
-
European Journal of Clinical Microbiology & Infectious Diseases
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373 - DOI
- https://doi.org/10.1007/s10096-025-05337-9
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