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16.08.2017 | Brief Report

Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium

verfasst von: Shaozhen Xing, Xianglilan Zhang, Qiang Sun, Jian Wang, Zhiqiang Mi, Guangqian Pei, Yong Huang, Xiaoping An, Kaifei Fu, Lijun Zhou, Baohua Zhao, Yigang Tong

Erschienen in: Archives of Virology | Ausgabe 12/2017

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Abstract

A novel virulent bacteriophage named vB_EfaP_IME199 that specifically infects Enterococcus faecium was isolated and characterized. Its optimal multiplicity of infection was 0.01, and it had a 30 minute outbreak period. High-throughput sequencing revealed that the phage has a dsDNA genome of 18,838 bp with 22 open reading frames. The genome has very low homology to all other bacteriophage sequences in the GenBank database. Run-off sequencing experiments confirmed that vB_EfaP_IME199 has short inverted terminal repeats. Phylogenetic analysis indicated that vB_EfaP_IME199 can be taxonomically classified as a new member of the genus Ahjdlikevirus of family Podoviridae.

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Titel: Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium
verfasst von: Shaozhen Xing
Xianglilan Zhang
Qiang Sun
Jian Wang
Zhiqiang Mi
Guangqian Pei
Yong Huang
Xiaoping An
Kaifei Fu
Lijun Zhou
Baohua Zhao
Yigang Tong
Publikationsdatum: 16.08.2017
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 12/2017
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3503-1

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Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium

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