Background
Methods
Mice
Behavior
Data analysis
Specific behaviors
Rotarod
Activity chamber
Elevated plus maze
Marble burying
Spontaneous alternation in T-maze
Olfactory dishabituation
Social novelty preference
Fear conditioning
Results and discussion
Metfx/fx/Emx1cre
Steady speed rotarod
Accelerating rotarod
WT | Metfx/fx/Emx1cre KO |
F or t Statistic |
p value | |
---|---|---|---|---|
Activity chamber | ||||
Genotype, F (1, 51) = 12.26 |
p < 0.05 | |||
Total distance traveled(cm) | 3317.67 ± 218.98 | 2339.74 ± 175.11 | Time, F (5, 255) = 4.308 |
p < 0.001 |
Interaction, F (5, 255) = 3.184 |
p < 0.01 | |||
Steady speed rotarod | ||||
Genotype, F (1, 52) = 4.249 |
p < 0.05 | |||
Average latency to fall(sec) | 20.0185 ± 2.58 | 28.481 ± 3.19 | Trial, F (9, 468) = 18.99 |
p < 0.0001 |
Interaction, F (9, 468) = 1.063 |
p > 0.05 | |||
Accelerating rotarod | ||||
Genotype, F (1, 12) = 0.017 |
p > 0.05 | |||
Average latency to fall(sec) | 151.43 ± 19.82 | 148.10 ± 16.14 | Trial, F (2, 24) = 15.35 |
p < 0.0001 |
Interaction, F (2, 24) = 0.024 |
p > 0.05 | |||
T-maze | ||||
% SA | 54.701 ± 1.679 | 47.4956 ± 2.401 |
t
51 = 2.475 |
p < 0.05 |
% AAR | 35.063 ± 1.554 | 38.576 ± 1.599 |
t
51 = −1.576 |
p > 0.05 |
% SAR | 3.891 ± 0.666 | 5.301 ± 0.907 |
t
51 = −1.410 |
p > 0.05 |
#arm entries | 35.852 ± 2.456 | 29.154 ± 1.859 |
t
51 = 2.162 |
p < 0.05 |
Marble burying (#) | 7.100 ± 1.567 | 9.200 ± 1.756 |
t
18 = 0.8923 |
p > 0.05 |
EPM entries (#) | ||||
Genotype, F (1, 32) = 0.2539 |
p > 0.05 | |||
Closed arms | 9.188 ± 1.697 | 8.882 ± 1.364 | Arm entry, F (1, 32) = 65.93 |
p < 0.05 |
Open arms | 1.706 ± 0.803 | 0.5882 ± 0.298 | Interaction, F (1, 32) = 0.2081 |
p > 0.05 |
EPM arm duration (%) | ||||
Genotype, F (1, 32) = 0.3463 |
p > 0.05 | |||
Closed arms | 88.882 ± 3.169 | 86.863 ± 5.017 | Arm duration, F (1, 32) = 220.8 |
p < 0.0001 |
Open arms | 4.3137 ± 1.505 | 3.000 ± 1.649 | Interaction, F (1, 32) = 0.2508 |
p > 0.05 |
Sociability (sec) | ||||
Genotype, F (1, 52) = 1.463 |
p > 0.05 | |||
Side with mouse | 312.897 ± 14.864 | 317.225 ± 11.721 | Chamber side, F (1, 52) = 74.25 |
p < 0.05 |
Side with empty cup | 185.271 ± 12.428 | 162.44 ± 11.885 | Interaction, F (1, 52) = 0.6864 |
p > 0.05 |
Social novelty (sec) | ||||
Genotype, F (1, 52) = 0.001 |
p > 0.05 | |||
Familiar mouse side | 227.609 ± 13.819 | 235.362 ± 17.602 | Chamber side, F (1, 52) = 1.545 |
p > 0.05 |
Novel mouse side | 259.723 ± 14.579 | 252.667 ± 14.533 | Interaction, F (1, 52) = 0.1387 |
p > 0.05 |
Sociability (sec) | ||||
Genotype, F (1, 52) = 0.005652 |
p > 0.05 | |||
Cup with mouse | 62.795 ± 4.653 | 60.152 ± 5.095 | Cup, F (1, 52) = 214.6 |
p < 0.05 |
Empty cup | 11.818 ± 1.606 | 12.645 ± 1.254 | Interaction, F (1, 52) = 0.2665 |
p > 0.05 |
Social novelty (sec) | ||||
Genotype, F (1, 52) = 0.02144 |
p > 0.05 | |||
Cup with familiar mouse | 23.087 ± 3.234 | 21.624 ± 2.801 | Cup, F (1, 52) = 63.83 |
p < 0.05 |
Cup with novel mouse | 46.756 ± 5.329 | 46.817 ± 4.221 | Interaction, F (1, 52) = 0.06198 |
p > 0.05 |
Contextual fear (%) | ||||
Baseline | 0.04976 ± 0.0497 | 0.3007 ± 0.1955 | Genotype, F (1, 31) = 0.6584 |
p > 0.05 |
Training | 28.73 ± 3.2649 | 32.72 ± 2.6444 | Trial, F (2, 62) = 110.5 |
p < 0.0001 |
Test | 29.04 ± 3.2954 | 30.81 ± 3.4346 | Interaction, F (2, 62) = 0.3224 |
p > 0.05 |
Activity chamber
Elevated plus maze
Marble burying
T-maze
Sociability and social novelty preference
Olfactory dishabituation
Contextual fear conditioning
Metfx/fx;fx/+/Nestincre
Steady speed rotarod
WT | Metfx/+/Nestincre Het | Metfx/fx/Nestincre KO |
F Statistic |
p value | |
---|---|---|---|---|---|
Activity chamber | |||||
Genotype, F (2, 55) = 2.491 |
p > 0.05 | ||||
Total distance traveled(cm) | 2807.3 ± 197.9 | 3445.5 ± 236.0 | 2842.9 ± 140.5 | Time, F (5, 275) = 18.09 |
p < 0.0001 |
Interaction, F (5, 275) = 1.410 |
p > 0.05 | ||||
Steady speed rotarod | |||||
Genotype, F (2, 55) = 1.971 |
p > 0.05 | ||||
Latency to fall(sec) | 27.417 ± 2.635 | 14.271 ± 2.016 | 20.033 ± 3.363 | Trial, F (9, 495) = 9.686 |
p < 0.0001 |
Interaction, F (9, 495) = 1.002 |
p > 0.05 | ||||
Accelerating rotarod | |||||
Genotype, F (2, 17) = 0.033 |
p > 0.05 | ||||
Latency to fall(sec) | 150.39 ± 15.80 | 155.49 ± 14.76 | 148.38 ± 14.71 | Trial, F (2, 34) = 18.50 |
p < 0.0001 |
Interaction, F (2, 34) = 1.737 |
p > 0.05 | ||||
T-maze | |||||
% SA | 44.837 ± 2.456 | 49.408 ± 2.568 | 50.113 ± 2.181 |
F (2, 53) = 1.417 |
p > 0.05 |
% AAR | 36.794 ± 2.114 | 31.852 ± 1.356 | 31.123 ± 2.096 |
F (2, 53) = 2.408 |
p > 0.05 |
% SAR | 7.354 ± 1.1011 | 7.840 ± 1.198 | 8.192 ± 1.170 |
F (2, 53) = 0.1350 |
p > 0.05 |
#arm entries | 27.963 ± 1.778 | 30.429 ± 2.467 | 33.333 ± 2.131 |
F (2, 53) = 1.742 |
p > 0.05 |
Marble burying (#) | 8 ± 1.076 | 10 ± 1.742 | 7.0 ± 1.366 |
F (2, 53) = 0.6750 |
p > 0.05 |
EPM entries (#) | |||||
Genotype, F (2, 54) = 1.490 |
p > 0.05 | ||||
Closed arms | 9.5 ± 0.946 | 11.21 ± 1.085 | 9.8 ± 1.147 | Arm entries, F (1, 54) = 147.8 |
p < 0.0001 |
Open arms | 1.75 ± 0.413 | 3.21 ± 0.594 | 2.4 ± 0.523 | Interaction, F (2, 54) = 0.06371 |
p > 0.05 |
EPM duration (%) | |||||
Genotype, F (2, 54) = 0.0 |
p > 0.05 | ||||
Closed arms | 81.037 ± 2.725 | 63.268 ± 5.701 | 75.057 ± 3.494 | Arm duration, F (2, 108) = 228.1 |
p < 0.0001 |
Open arms | 8.102 ± 2.276 | 13.523 ± 5.628 | 8.736 ± 2.077 | Interaction, F (2, 108) = 4.299 |
p < 0.005 |
Sociability (sec) | |||||
Genotype, F (2, 53) = 1.032 |
p > 0.05 | ||||
Side with mouse | 335.257 ± 12.743 | 340.71 ± 11.666 | 310.944 ± 13.768 | Chamber side, F (2, 106) = 197.6 |
p < 0.0001 |
Side with empty cup | 165.806 ± 10.710 | 144.05 ± 10.953 | 199.303 ± 14.887 | Interaction, F (2, 106) = 2.493 |
p < 0.05 |
Social novelty (sec) | |||||
Genotype, F (2, 53) = 0.4669 |
p > 0.05 | ||||
Familiar mouse side | 239.276 ± 15.473 | 275.184 ± 20.999 | 239.899 ± 24.126 | Chamber side, F (2, 106) = 47.53 |
p < 0.0001 |
Novel mouse side | 256.915 ± 14.165 | 213.617 ± 17.351 | 256.977 ± 24.069 | Interaction, F (2, 106) = 1.174 |
p > 0.05 |
Sociability (sec) | |||||
Genotype, F (2, 53) = 0.7821 |
p > 0.05 | ||||
Cup with mouse | 76.390 ± 5.722 | 72.092 ± 5.533 | 64.901 ± 5.795 | Cup time, F (1, 53) = 212.2 |
p < 0.0001 |
Empty cup | 13.486 ± 1.589 | 12.605 ± 2.816 | 14.391 ± 2.338 | Interaction, F (2, 53) = 0.9274 |
p > 0.05 |
Social novelty (sec) | |||||
Genotype, F (2, 53) = 0.2960 |
p > 0.05 | ||||
Cup with familiar mouse | 25.686 ± 3.224 | 27.324 ± 3.362 | 25.807 ± 3.938 | Cup time, F (1, 53) = 23.81 |
p < 0.0001 |
Cup with novel Mouse | 46.11 ± 3.858 | 38.358 ± 4.95 | 41.836 ± 4.558 | Interaction, F (2, 53) = 0.7666 |
p > 0.05 |
Cue fear training (%) | |||||
Genotype, F (2, 21) = 1.206 |
p > 0.05 | ||||
Average freezing | 27.681 ± 3.621 | 20.959 ± 9.090 | 24.956 ± 2.421 | Trial, F (4, 84) = 31.76 |
p < 0.0001 |
Interaction, F (4, 84) = 0.8341 |
p > 0.05 | ||||
Cue fear context test (%) | |||||
Average freezing | 25.501 ± 2.778 | 21.132 ± 6.485 | 24.408 ± 4.978 |
F (2, 21) = 0.201 |
p > 0.05 |
Cue fear cue test (%) | |||||
Genotype, F (2, 21) = 3.156 |
p > 0.05 | ||||
Average freezing | 61.120 ± 1.062 | 72.072 ± 2.478 | 78.351 ± 2.758 | Trial, F (9, 189) = 1.398 |
p > 0.05 |
Interaction, F (9, 189) = 0.9551 |
p > 0.05 | ||||
Cue fear extinction 1 (%) | |||||
Genotype, F (2, 21) = 1.032 |
p > 0.05 | ||||
Average freezing | 47.999 ± 1.515 | 60.181 ± 2.219 | 56.860 ± 2.118 | Trial, F (9, 189) = 1.975 |
p < 0.05 |
Interaction, F (9, 189) = 0.6945 |
p > 0.05 | ||||
Extinction 2 | |||||
Genotype, F (2, 21) = 0.9524 |
p > 0.05 | ||||
Average freezing | 34.572 ± 1.967 | 40.725 ± 3.196 | 45.103 ± 1.575 | Trial, F (9, 189) = 1.480 |
p > 0.05 |
Interaction, F (9, 189) = 1.196 |
p > 0.05 | ||||
Extinction 3 | |||||
Genotype, F (2, 21) = 0.2070 |
p > 0.05 | ||||
Average freezing | 30.900 ± 2.513 | 29.381 ± 1.754 | 36.167 ± 1.686 | Trial, F (9, 189) = 1.026 |
p > 0.05 |
Interaction, F (9, 189) = 1.180 |
p > 0.05 |