Background
Methods
Animals
Physical exercise protocol
EAE induction and clinical assessment
Gait analysis
Time point of analysis | EAE mice | EAE-Exercised mice |
---|---|---|
Basal | 12 (100%) | 12 (100%) |
5 dpi | 12 (100%) | 12 (100%) |
12 dpi | 12 (100%) | 12 (100%) |
19 dpi | 07 (58%) | 04 (33%) |
22 dpi | 10 (83%) | 08 (67%) |
25 dpi | 08 (67%) | 05 (42%) |
28 dpi | 10 (83%) | 07 (58%) |
31 dpi | 06 (75%) | 05 (63%) |
36 dpi | 07 (88%) | 04 (50%) |
42 dpi | 07 (88%) | 04 (50%) |
Rotarod
Statistical analysis
Results
Regular treadmill exercise previously to EAE onset does not influence clinical score, rotarod data, and speed of the walking in EAE mice
Parameters
|
EAE
|
EAE-Ex
| ||||
---|---|---|---|---|---|---|
CS
|
RD
|
R
|
CS
|
RD
|
R
| |
Clinical score | − | 0.73#
| −0.73#
| − | 0.72#
| −0.77#
|
Run Duration (s) | 0.73#
| − | −0.65#
| 0.72#
| − | −0.64#
|
Rotarod (s) | −0.73#
| −0.65#
| − | −0.77#
| −0.64#
| − |
Run Average Speed (cm/s) | −0.65#
| −0.87#
| 0.60#
| −0.73#
| −0.95#
| 0.59#
|
Cadence (steps/s) | −0.67#
| −0.88#
| 0.61#
| −0.66#
| −0.93#
| 0.60#
|
Regularity Index (%) | −0.65#
| −0.67#
| 0.59#
| −0.75#
| −0.69#
| 0.58#
|
Phase Dispersions RF → LH | 0.35+
| 0.38+
| −0.34+
| 0.64#
| 0.51#
| −0.34+
|
Phase Dispersions LF → RH | 0.32+
| 0.29+
| −0.22*
| 0.61#
| 0.55#
| −0.38+
|
Couplings RF → LH | −0.69#
| −0.67#
| 0.62#
| −0.54#
| −0.45+
| 0.32+
|
Couplings LF → RH | −0.63#
| −0.50#
| 0.47#
| −0.51#
| −0.44+
| 0.28*
|
Diagonal Support (%) | −0.62#
| −0.73#
| 0.48#
| −0.62#
| −0.75#
| 0.39+
|
Lateral Support (%) | 0.51#
| 0.33+
| −0.42#
| 0.71#
| 0.35+
| −0.40+
|
Three Support (%) | 0.36+
| 0.59#
| −0.21*
| 0.38+
| 0.66#
| −0.27*
|
Girdle Support (%) | 0.35+
| 0.39+
| −0.43#
| 0.30*
| 0.20 | −0.22 |
Zero Support (%) | 0.17 | 0.01 | −0.08 | 0.41+
| −0.01 | −0.27*
|
EAE animals present decreased inter-paw coordination, and prior exercise seems to modify the style of support during the progression of the disease
Differential modulation of dynamic and static gait parameters of front (FP) and hind paws (HP) in EAE and exercised EAE (EAE-ex) animals
Parameters | Front paws (FP) | Hind paws (HP) | ||||||
---|---|---|---|---|---|---|---|---|
EAE | EAE-Ex | EAE | EAE-Ex | |||||
CS | RD | CS | RD | CS | RD | CS | RD | |
Stride Length (cm) | −0.60#
| −0.84#
| −0.74#
| −0.80#
| −0.72#
| −0.85#
| −0.68#
| −0.78#
|
Body Speed (cm/s) | −0.67#
| −0.89#
| −0.74#
| −0.95#
| −0.69#
| −0.89#
| −0.75#
| −0.95#
|
Swing Speed (cm/s) | −0.51#
| −0.71#
| −0.42+
| −0.63#
| −0.73#
| −0.85#
| −0.75#
| −0.83#
|
Step Cycle (s) | 0.64#
| 0.82#
| 0.45#
| 0.77#
| 0.58#
| 0.88#
| 0.44#
| 0.73#
|
Stand (s) | 0.70#
| 0.93#
| 0.57#
| 0.88#
| 0.18 | 0.43#
| 0.06 | 0.45#
|
Duty Cycle (%) | 0.56#
| 0.81#
| 0.64#
| 0.85#
| −0.30+
| −0.14 | −0.36+
| 0.02 |
Initial Dual Stance (s) | 0.66#
| 0.90#
| 0.63#
| 0.88#
| 0.07 | 0.37+
| 0.06 | 0.53#
|
Terminal Dual Stance (s) | 0.67#
| 0.90#
| 0.64#
| 0.87#
| 0.16 | 0.47#
| 0.11 | 0.57#
|
Base of Support (cm) | 0.58#
| 0.61#
| 0.72#
| 0.73#
| −0.31+
| −0.18 | −0.44+
| −0.20 |
Max Contact Max Intensity | −0.13 | 0.15 | −0.46#
| −0.15 | −0.55#
| −0.36+
| −0.76#
| −0.47#
|
15 Most Intense Pixels | −0.08 | 0.20 | −0.31+
| −0.02 | −0.53#
| −0.28+
| −0.73#
| −0.46#
|
Max Intensity At (%) | 0.51#
| 0.40#
| 0.52#
| 0.43#
| −0.64#
| −0.37+
| −0.73#
| −0.54#
|
Max Contact At (%) | 0.25*
| 0.38#
| 0.44#
| 0.58#
| −0.44#
| −0.39+
| −0.25*
| −0.27*
|
Print Width (cm) | −0.40#
| −0.28+
| −0.66#
| −0.42+
| −0.51#
| −0.31+
| −0.41+
| −0.20 |
Print Length (cm) | −0.16 | −0.02 | −0.29*
| −0.07 | −0.18 | −0.03 | −0.26*
| −0.09 |
Print Area (cm2) | −0.15 | 0.11 | −0.36*
| −0.10 | −0.27*
| −0.05 | −0.45#
| −0.22 |