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Erschienen in: Journal of Cancer Research and Clinical Oncology 8/2003

01.08.2003 | Original Paper

Construction of an EGF receptor-mediated histone H10-based gene delivery system

verfasst von: Fei-Han Dai, Yan Chen, Chang-Chun Ren, Jin-Jun Li, Min Yao, Jun-Song Han, Yi Gong, Sheng-Li Yang, Jing-De Zhu, Jian-Ren Gu

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 8/2003

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Abstract

Purpose

To construct an EGF receptor (EGF-R)-mediated histone H10-based gene delivery system for gene therapy.

Methods

A recombinant DNA containing histone H10, EGF-R ligand, and endosomalytic domains was constructed in a prokaryotic vector and expressed in E. coli. Expression of the β-galactosidase (β-gal) gene in the tumor cells and tissues was observed after transduction of the β-gal gene packaged by purified fusion proteins in vitro and in vivo.

Results

As an extension of the research on previously reported chemically synthetic composite polypeptide gene delivery systems, this genetically engineered polypeptide has proved to be capable of targeting the β-galactosidase (β-gal) gene into EGF-R-positive cancer cells both in vitro and in vivo. We also studied the time course of β-gal gene expression in tumor tissues delivered in vivo by this polypeptide vector. At 24 h after administration, expression of the β-galactosidase gene in tumor reached peak levels. The dosage optimization of administered polyplex was also investigated. The optimal dose of polyplex per mouse was 1 μg DNA packaged by 3 μg of composite polypeptide.

Conclusions

The genetically engineered polypeptide based on histone H10 is a promising gene delivery system targeting EGF-R.
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Metadaten
Titel
Construction of an EGF receptor-mediated histone H10-based gene delivery system
verfasst von
Fei-Han Dai
Yan Chen
Chang-Chun Ren
Jin-Jun Li
Min Yao
Jun-Song Han
Yi Gong
Sheng-Li Yang
Jing-De Zhu
Jian-Ren Gu
Publikationsdatum
01.08.2003
Verlag
Springer-Verlag
Erschienen in
Journal of Cancer Research and Clinical Oncology / Ausgabe 8/2003
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-003-0452-8

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