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26.02.2019 | Original Research Article

Corosolic acid: antiangiogenic activity and safety of intravitreal injection in rats eyes

verfasst von: Cibele Rodrigues Toledo, Vinícius Viana Pereira, Lays Fernanda Nunes Dourado, Mayara Rodrigues Brandão Paiva, Armando Silva-Cunha

Erschienen in: Documenta Ophthalmologica | Ausgabe 3/2019

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Abstract

Purpose

Investigate the potential application of corosolic acid (CA) in the treatment of diseases causing retinal neovascularization.

Methods

CA cytotoxicity effect was evaluated in ARPE-19 cells by sulforhodamine B colorimetric method, and antiangiogenic activity was studied using chorioallantoic membrane (CAM) assay. An amount of 0.01 mL of CA formulations at 5, 10 and 25 µM was injected in the right eyes of Wistar rats, and the contralateral eyes received the vehicle to verify the safety of ophthalmic use. Electroretinography (ERG) was performed before, 7 and 15 days after CA administration. Animals were killed on the 15th day, and the histological analysis of retina was carried out under light microscopy.

Results

CA did not present cytotoxicity at concentrations below 35.5 μM after 48 h of treatment. The antiangiogenic activity was confirmed by CAM assay, since CA (range from 5 to 25 µM) induced a significant reduction in vascularity without any signs of toxicity. ERG recordings and histological evaluation did not show any signs of retinal toxicity.

Conclusions

CA was effective in reducing vascularity in a CAM model and was found to be safe for potential ophthalmic use.

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Titel: Corosolic acid: antiangiogenic activity and safety of intravitreal injection in rats eyes
verfasst von: Cibele Rodrigues Toledo
Vinícius Viana Pereira
Lays Fernanda Nunes Dourado
Mayara Rodrigues Brandão Paiva
Armando Silva-Cunha
Publikationsdatum: 26.02.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Documenta Ophthalmologica / Ausgabe 3/2019
Print ISSN: 0012-4486
Elektronische ISSN: 1573-2622
DOI: https://doi.org/10.1007/s10633-019-09682-x

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Springer Medizin

Corosolic acid: antiangiogenic activity and safety of intravitreal injection in rats eyes

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Augenheilkunde

Ophthalmika in der Schwangerschaft

Operative Therapie und Keimnachweis bei endogener Endophthalmitis

Bakterielle endogene Endophthalmitis

Bestmögliche Wundheilung der Kornea dank optimaler pharmakologischer Therapie

Update Augenheilkunde

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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