Background
Methods
Study subjects
Data collection
Sample and genotyping assay of TLRs polymorphisms
Determination of HbsAg serum level
Serum level of TNF-α (tumor necrosis factor-α) and IL-6 (Interleukin-6) assay
Statistical analysis
Results
Characterization of study subjects
Variables | Mild chronic hepatitis | Moderate and severe chronic hepatitis | P value |
---|---|---|---|
Number (%) | Number (%) | ||
Gender | |||
Male | 93(61.6) | 50(83.3) | |
Female | 58(38.4) | 10(16.7) | 0.002a |
Ageb | 30.70(7.09) | 31.10(7.86) | 0.717 |
BMIb | 21.40(2.94) | 22.36(3.37) | 0.040a |
Marital Status | |||
Single | 40(26.5) | 19(31.7) | |
Married | 108(71.5) | 41(68.3) | |
Divorced | 3(2.0) | 0(0.0) | 0.513 |
Smoking Status | |||
Smokers | 17(11.3) | 13(21.7) | |
Never-smokers | 134(88.7) | 47(78.3) | 0.051 |
Alcohol consumption | |||
No | 20(13.2) | 14(23.3) | |
Yes | 131(86.8) | 46(76.7) | 0.072 |
Family history of hepatitis B | |||
No | 95(62.9) | 47(78.3) | |
Yes | 56(37.1) | 13(21.7) | 0.031a |
HBV genotype | |||
Genotype B | 123(81.5) | 48(80.0) | |
Genotype C | 28(18.5) | 12(20.0) | 0.808 |
TLR polymorphisms and hepatitis activity
Genotype | Mild chronic hepatitis | Moderate and severe chronic hepatitis | Odds Ratio (95%CI) |
---|---|---|---|
Number (%) | Number (%) | ||
rs1075993 | |||
TT | 83(55.0) | 25(41.7) | 1(Ref.) |
CT | 64(42.4) | 32(53.3) | 1.66(0.90–3.08) |
CC | 4(2.6) | 3(5.0) | 2.49(0.52–11.88) |
rs3804099 | |||
TT | 60(39.7) | 41(68.3) | 1(Ref.) |
CT | 50(33.1) | 15(25.0) | 0.44(0.22–0.89)a |
CC | 41(27.2) | 4(6.7) | 0.14(0.05–0.43)a |
rs4696480 | |||
CC | 83(55.0) | 25(41.7) | 1(Ref.) |
CT | 64(42.4) | 32(53.3) | 0.98(0.48–1.98) |
TT | 4(2.6) | 3(5.0) | 0.35(0.15–0.80)a |
Comparison of liver function parameters by TLR polymorphisms
Variables | rs10759932 wild type | rs10759932 mutant |
P
| rs3804099 wild type | rs3804099 mutant |
P
|
---|---|---|---|---|---|---|
Total bilirubin(umol/L) | 18.04(6.15) | 21.87(23.24) | 0.665 | 26.56(40.20) | 20.43(15.08) | 0.320 |
Direct bilirubin(umol/L) | 3.24(2.27) | 5.65(14.20) | 0.655 | 8.45(24.88) | 4.79(8.98) | 0.337 |
Indirect bilirubin(umol/L) | 14.80(5.29) | 16.98(11.51) | 0.618 | 19.98(19.24) | 16.08(7.90) | 0.337 |
ALT(U/L) | 187.00(216.38) | 196.13(212.91) | 0.911 | 340.91(362.01) | 156.50(123.43) | 0.002a |
AST(U/L) | 220.57(228.28) | 162.275(170.61) | 0.380 | 231.42(267.27) | 145.99(131.27) | 0.043a |
Total protein(g/L) | 78.00(5.26) | 73.79(6.45) | 0.090 | 72.44(11.15) | 74.34(4.36) | 0.270 |
Globulin(g/L) | 45.43(5.00) | 41.86(4.35) | 0.035a | 41.98(7.26) | 42.30(3.27) | 0.996 |
Albumin(g/L) | 32.57(3.87) | 32.11(4.13) | 0.772 | 31.27(3.50) | 32.36(4.24) | 0.080 |
Variables | rs4696480 wild type | rs4696480 mutant |
P
|
---|---|---|---|
Total bilirubin(umol/L) | 21.29 ± 20.31 | 21.96 ± 24.13 | 0.840 |
Direct bilirubin(umol/L) | 5.47 ± 12.62 | 5.62 ± 14.64 | 0.941 |
Indirect bilirubin(umol/L) | 17.33 ± 12.60 | 16.70 ± 10.73 | 0.706 |
ALT(U/L) | 244.61 ± 198.66 | 171.61 ± 215.66 | 0.018a |
AST(U/L) | 152.33 ± 190.18 | 170.11 ± 163.32 | 0.482 |
Total protein(g/L) | 73.39 ± 4.88 | 74.21 ± 7.10 | 0.386 |
Globulin(g/L) | 42.53 ± 3.27 | 41.71 ± 4.86 | 0.204 |
Albumin(g/L) | 30.86 ± 4.09 | 32.76 ± 3.99 | 0.001a |
Comparison of HbsAg level by TLR polymorphisms
Genotypes | Normalized HbsAg level (ng/ml) |
---|---|
rs1075993 wild type | 4.69(0.57) |
rs1075993 mutant | 4.11(0.77) |
P
| 0.051 |
rs3804099 wild type | 4.37(0.78) |
rs3804099 mutant | 4.07(0.76) |
P
| 0.014a |
rs4696480 wild type | 4.35(0.80) |
rs4696480 mutant | 4.02(0.73) |
P
| 0.003a |
Comparison of TNF-α and IL-6 serum level by TLR2 polymorphisms
Genotypes | TNF- α(pg/ml) | IL-6(pg/ml) |
---|---|---|
rs1075993 wild type | 2.94 ± 1.48 | 6.18 ± 2.47 |
rs1075993 mutant | 3.13 ± 1.34 | 5.87 ± 2.39 |
P
| 0.330 | 0.357 |
rs3804099 wild type | 3.13 ± 1.47 | 6.38 ± 2.13 |
rs3804099 mutant | 2.91 ± 1.35 | 5.66 ± 2.68 |
P
| 0.262 | 0.031a |
rs4696480 wild type | 3.39 ± 1.60 | 6.71 ± 2.73 |
rs4696480 mutant | 2.66 ± 1.09 | 5.36 ± 1.86 |
P
| <0.001 a | <0.001a |