Background
Materials and methods
Antibodies and reagents
Patients and human tissue specimens
Semi-quantitative RT-PCR
Western blot
Immunohistochemical analysis and scoring
Cell lines and shRNA transfection
Wound healing assay, cell invasion and migration analysis
Immunofluorescence
Statistical analysis
Results
CBX8 expression is associated with HCC metastasis
Knock-down of CBX8 inhibits metastasis of HCC cells
CBX8 induces EMT process
Correlations between CBX8 and EMT associated factors
HCC tissues | n | Adjacent non-tumor tissues | P value | |
---|---|---|---|---|
High | Low | |||
E-cadherin | ||||
High | 54 | 23 | 31 | 0.008 |
Low | 112 | 72 | 40 | |
β-catenin | ||||
High | 69 | 50 | 19 | 0.001 |
Low | 97 | 46 | 51 | |
N-cadherin | ||||
High | 79 | 33 | 46 | 0.005 |
Low | 87 | 22 | 65 | |
Vimentin | ||||
High | 117 | 56 | 61 | 0.000 |
Low | 49 | 15 | 34 | |
ZEB1 | ||||
High | 121 | 67 | 54 | 0.000 |
Low | 45 | 17 | 28 | |
ZEB2 | ||||
High | 102 | 48 | 54 | 0.000 |
Low | 65 | 20 | 45 |
EMT factors | n | CBX8 | χ2 value | r value | P value | |
---|---|---|---|---|---|---|
High | Low | |||||
E-cadherin | ||||||
High | 54 | 9 | 45 | 33.066 | − 0.446 | 0.000 |
Low | 112 | 72 | 40 | |||
β-catenin | ||||||
High | 69 | 12 | 57 | 46.608 | − 0.530 | 0.000 |
Low | 97 | 69 | 28 | |||
N-cadherin | ||||||
High | 79 | 65 | 14 | 67.637 | 0.638 | 0.005 |
Low | 87 | 16 | 71 | |||
Vimentin | ||||||
High | 117 | 75 | 42 | 37.172 | 0.473 | 0.000 |
Low | 49 | 6 | 43 | |||
ZEB1 | ||||||
High | 121 | 71 | 50 | 17.447 | 0.324 | 0.000 |
Low | 45 | 10 | 35 | |||
ZEB2 | ||||||
High | 102 | 66 | 36 | 26.805 | 0.402 | 0.000 |
Low | 64 | 15 | 49 |