Cortical diffusivity investigation in posterior cortical atrophy and typical Alzheimer’s disease

All subjects’ data in the study had been collected as part of previous studies. Fifteen PCA patients were recruited through the Oxford Cognitive Disorders Clinic, Oxford, UK. Diagnosis was established by a senior behavioural neurologist (CB) and neuropsychologist (SA). All patients fulfilled consensus criteria for PCA [4, 5], based upon clinical assessment, brain imaging and detailed neuropsychological assessment. Clinical magnetic resonance imaging (MRI) confirmed focal atrophy in the occipital and parietal lobes.

A tAD and a control group were used for comparisons with the PCA patients. Twenty-three tAD patients were recruited from the Oxford Project to Investigate Memory and Aging (OPTIMA) [28] and the Memory and Amnesia Project, University of Oxford, UK. All tAD subjects fulfilled consensus criteria for Alzheimer’s disease [29, 30], based upon clinical assessment, detailed neuropsychological assessment, and structural brain imaging.

Twenty-two comparable healthy controls were recruited in Oxford. Participants were recruited from the OPTIMA Project and from the Oxford Memory Assessment Clinic (OXMAC) at the John Radcliffe Hospital. Healthy volunteers were recruited as such if they did not have a subjective or reported memory complaint and performed within the normal range in global cognitive scales (i.e., MMSE > 26 and CDR = 0). These participants had no prior history of psychiatric illness, significant head injury, or cerebrovascular disease, and were not prescribed any medication known to affect cognition. Neuropsychological data were not comparable between groups due to the earlier acquisition of some data under different research protocols.

Scanning for all subjects entered in the study was performed at the Oxford Centre for Clinical Magnetic Resonance Research using a 3 T Trio Siemens MRI scanner equipped with a 12-channel head coil. The neuroimaging protocol included: (1) diffusion weighted image (DWI) acquisition SE-EPI sequence, TR/TE = 9300/94 ms; resolution = 2 × 2 × 2 mm³; flip angle 90°; FOV = 192 mm; number of diffusion directions = 60; b value = 1000 s/mm² and two additional images with no diffusion weighting (b = 0), (2) high-resolution T1-weighted 3D MP-RAGE images (TR/TE = 2040/4.7 ms; resolution = 1 × 1 × 1 mm³; FOV 192 mm).

The 3D T1-weighted image for each subject was segmented using FreeSurfer v 6.0 (https://​surfer.​nmr.​mgh.​harvard.​edu/​). This provided outputs containing estimates of the cortical grey matter volume, white matter (WM) volume, cortical surfaces (CS) and the cortical thickness (CT), based on the standard segmentation and surface-fitting performed by FreeSurfer.

DTI preprocessing was performed using FSL tools (FSL Version 6.0; FMRIB Software Library, Oxford, UK—https://​www.​fmrib.​ox.​ac.​uk/​fsl/​). For each subject, the diffusion data were visually checked by two trained investigators (MT and SA) to detect artefacts and corrupted volumes. DTI scans of low quality were removed (1 AD and 1 PCA). Diffusion-weighted images were then corrected for motion and eddy current effects by alignment of all images to a reference b = 0 image using FSL’s eddy tool. The diffusion tensor was then calculated with the FSL DTIFIT tool, providing fractional anisotropy (FA), mean diffusivity (MD) and V1 maps. For each subject, the displacement among diffusion volumes was estimated using the eddy_correct output to obtain a measure of head movement (defined as “movement”) during the acquisition. To reduce the impact of partial volume effect, we used a 0.5 mm erosion from the edges. In previous testing, we explored four different degrees of erosion and 0.5 mm was selected as the best combination between reduction of partial volume effect and maintaining a sufficient number of voxels. The tests showed that any remaining partial volume effect due to contamination from CSF and WM, had little impact on the cortical diffusion measures.

Standard diffusivity analysis was conducted to calculate MD and FA in the cortex. Further cortical diffusivity analysis was performed using a proprietary software tool. The tool generates cortical profiles, i.e., radial lines within the cortex, providing an estimate of the columnar organisation within the cortex. Values for the diffusion tensor derived metrics were averaged along the cortical profiles, throughout cortical grey matter (method previously described) [21]. To summarise the method, three measures were calculated in this manner, relating to the principal diffusion component: the angle between the radial minicolumnar direction and the principal diffusion direction (AngleR, θrad); the principal diffusion component perpendicular to the radial minicolumnar direction [PerpPD, D1,⊥ (× 10^–3 mm²/s)], and the principal diffusion component parallel with the radial minicolumnar direction [ParlPD, D1,∥ (× 10^–3 mm²/s)] (see also the article by McKavanagh et al. [21] where AngleR was described as the difference between the radial minicolumn direction in the cortex and the principal diffusion vector, PerpPD and ParlPD were calculated by projecting the principal vector diffusion tensor component on to the planes perpendicular and parallel to the radial minicolumn direction in the cortex). All the cortical values were averaged across all of the cortical profiles within the region of interest (being the entire cortex for the whole brain value, or the cortical anatomical area for the regional values) to reduce the influence of noise in the DTI scans. This effectively smoothed the data, ensuring only directionality with some local coherence would dominate, guarding against the influence of random deflections from the radial direction. Previous work has found that measures of the cyto- and myelo-architecture are relatively stable within a cortical subregion [31] indicating that it is valid to find an average value for that region. Global cortical diffusion values included all regions of the cerebral cortex to generate overall values for the whole brain cerebral cortex. These were used to compare groups and the metrics that differentiated the three groups were then used for the regional analyses. To investigate the regional cortical diffusion metrics, single values for each cortical region were extracted using the regional segmentation provided by the recon-all pipeline of the FreeSurfer v 6.0 software package (https://​surfer.​nmr.​mgh.​harvard.​edu/​) based on Desikan-Atlas.

The cortical GM mask, the WM mask and the CS mask obtained by Freesurfer recon-all were used to estimate the GM and WM volume. To account for head size, the GM, WM and CS volumes were normalised by the total intracranial volume (TIV) to obtain the GM fraction GM_fr,WM fraction (WM_fr) and CS fraction (CS_fr).

The right and left hippocampal volumes were automatically segmented using Freesurfer recon-all. Hippocampal volumes (left and right) were averaged and normalised by TIV to obtain the hippocampal fraction (Hipp_fr).

ANOVA was performed using the multivariate general linear model (GLM) in SPSS to compare the between-group differences in diffusion and volumetric measurements, using the diagnostic group as a fixed factor and the head movement and age as covariates. To investigate the associations between global cortical diffusivity, volume, and thickness, Partial Spearman’s rank correlations were used, accounting for age and gender.

All statistically significant results reported remained significant after false discovery rate correction (FDR < 0.05) [32]. Bonferroni correction was used to account for multiple comparisons in demographics (p < 0.05/9).

To investigate the discrimination power of the cortical diffusion measures (FA, MD, AngleR, PerpPD and ParlPD) and three conventional structural measures (GM_fr, CT and Hipp_fr), the Receiver operating characteristic (ROC) curves were used. Every measure entered into the ROC analysis was adjusted for age and head movement. The area under the curve was used as a diagnostic accuracy index.

Table 1 summarises the principal demographic and clinical characteristics of all subjects entered in the study. No significant difference was observed between groups for years of formal education (F_2,55 = 2.84; p = 0.07) or sex (Chi-square HC vs tAD = 0.0178; p = 0.89.; HC vs PCA = 0.1081; p = 0.74.; tAD vs PCA = 0.0451; p = 0.83). The PCA group was significantly younger than HC group (F_2,55 = 9.47; p = 0.000). The illness duration was higher in the PCA group compared to tAD (F_2,36 = 8.151; p = 0.048), but this was not significant after multiple comparisons correction. Age and movement were used as covariates in further GLM analysis.

For global cortical volumetric measures, GLM analysis revealed a significant effect of diagnostic group on GM_fr (F_2,58 = 32.679; p = 0.000), WM_fr (F_2,58 = 6.222; p = 0.004), CT (F_2,58 = 14.800; p = 0.000) and Hipp_fr (F_2,58 = 18.539; p = 0.000). The between groups comparisons are summarized in Table 1. No significant effects of age or movement on volumetric variables were detected.

Cortical regional comparisons are summarized in Tables 3 and 4 and shown in Figs. 1 and 2 (see also Supplemental file). The GLM analysis revealed multiple significantly different regional PerpPD and ParlPD values in both hemispheres.

As expected, the between groups comparisons (Tables 3, 4) revealed that both patient groups exhibited higher PerpPD and ParlPD values compared to the HC group in a large number of frontal, temporal, parietal and occipital cortical regions. Comparing directly the two patient groups, the PCA group revealed higher PerpPD and ParlPD values in a large number of parieto-occipital regions (e.g., inferior parietal, lateral occipital, lingual, precuneus, superior parietal and supramarginal cortex), and the tAD group had higher PerpPD in the left entorhinal cortex and higher ParlPD in bilateral entorhinal cortex (FDR < 0.05; 210 tests).

The results showed significant differences between all groups for the PerpPD and ParlPD global values, with the PCA group presenting the higher values. Based on previous studies [21, 33, 34], these measures and other, related, cortical diffusivity values may act as surrogate measures of cytoarchitectural features such as minicolumn structure and may also be sensitive to changes in organization related to neuropathology including pathological protein accumulation.

Neurodegeneration in Alzheimer’s disease (and other dementias) proceeds insidiously and gradually from synaptic damage to dendrite loss, to cell loss and then to large scale atrophy. With respect to cytoarchitectural organisation there is shrinkage of horizontal cortical layers associated with cortical thinning and there is shrinkage and disruption of vertical minicolumn organisation. One concept in previous work [25] is that the minicolumn width shrinks initially due to loss of neuropil between cells, and then, in severe AD, there is minicolumn breakdown due to tangle accumulation and cell loss. It is possible that these phases of progression could be associated with differences in the characteristics of diffusion in the cortex due to changes in microscopic geometry of cytoarchitecture [35]. Furthermore, it appears that the different patterns of neurodegeneration between PCA and AD may be reflected in some measures of cortical diffusivity more than others, in this case in differences in PerpPD and ParlPD.

The global grey matter fraction and cortical surface area were reduced in both patient groups compared to the healthy control group, with no significant differences between tAD and PCA groups. However, the PCA group also had a reduced cortical thickness compared to the tAD group. Therefore, both of the contributing components of cortical volume (surface area and thickness) were affected in PCA and to some extent worse that in tAD. This is consistent with more severe changes that may be due to the longer illness duration of PCA. Illness duration was also correlated with PerpPD and ParlPD values across this dataset. The additional finding that cortical thickness was negatively correlated with global cortical PerpPD and ParlPD suggests there may be a meaningful coincidence of longer duration of illness and more severe cortical thickness and cortical diffusivity damage in PCA.

Consistent with previous works using other forms of assessment [13, 17, 36], the regional comparisons revealed that the PCA and tAD subgroups showed two different patterns of cortical changes (Fig. 3). Compared with healthy controls, PCA patients showed significantly higher values of PerpPD and ParlPD in bilateral parietal and occipital regions (e.g., inferior parietal, lateral occipital, lingual, superior parietal, supramarginal, precuneus). In contrast, tAD patients demonstrated significantly higher PerpPD and ParlPD values, compared with controls, in temporal regions (e.g., entorhinal, middle temporal, parahippocampal, superior temporal, transverse temporal, insula). Comparing the two patient groups directly, tAD patients had higher PerpPD in left entorhinal cortex and higher ParlPD values in bilateral entorhinal cortex than PCA patients. As is well known, the entorhinal region is the earliest one affected in tAD neurodegeneration [13, 37], contributing to the memory symptoms reported in tAD.

Compared with tAD patients, PCA patients had higher PerpPD and ParlPD values mainly in some posterior cortical regions, usually related to visuospatial and visuoperceptual abilities, encompassing bilateral fusiform, bilateral cuneus, bilateral inferior parietal, bilateral lateral occipital, bilateral lingual, bilateral precuneus, bilateral superior parietal, right paracentral and right supramarginal cortex. As shown in other studies [38, 39], these regions are involved in visual perception tasks and their damage can cause the deficits that characterize the clinical presentation of PCA.

This different pattern of cortical changes seems to be confirmed also by volumetric comparisons in which both patient groups showed a lower bilateral hippocampal fraction compared to healthy controls but also a lower bilateral hippocampal fraction in tAD compared with PCA.

Concerning the discrimination power of these cortical diffusivity measures, this study demonstrated that the global measures enabled differentiation with excellent accuracy between HC and all patients grouped together (AngleR AUC = 0.902), between HC and tAD (AngleR = 0.922) and between HC and PCA (PerpPD AUC = 0.961). However, to differentiate between the two patient groups the global cortical volumetric and diffusion measures were of limited use [ParlPD demonstrated the best AUC (0.771)].

According to previous studies [40‐42], tAD and PCA could have the same underlying neuropathology, but a different cortical distribution, therefore, a global cortical measure may not be suitable, or sensitive enough, to discriminate between the two dementia groups.

The regional cortical values were found to differentiate tAD and PCA patients with a higher discrimination accuracy than global cortical values. The best AUC was shown by PerpPD right lingual cortex (AUC = 0.856), PerpPD right superior parietal cortex (AUC = 0.842) and ParlPD right lateral occipital cortex (AUC = 0.826). These regions are involved in visual-spatial tasks and represent key regions in the PCA cortical signature.

These findings further confirm an association between the novel cortical diffusivity measurements and the cortical region size changes observed in both PCA and tAD patients, suggesting a potential role for DTI measures to enhance assessment of cortical changes in dementia.

The novel cortical diffusion measures described could have several applications. Further studies will enable a greater understanding of the underlying pathological cortical changes in the brain caused by various neurodegenerative diseases. Novel cortical diffusion measures may prove to be useful for investigating the cytoarchitectural organization changes among dementia types and could be an effective tool to aid differential diagnosis.

Despite the novel findings described above, there are several limitations to the current study. First, the study included a relatively small number of subjects because of the rarity of PCA, therefore these findings should be replicated with a larger number of subjects. Additionally, no neuropsychological comparisons between groups were possible because the two patient groups were originally enrolled in two different projects using different batteries of neuropsychological tools. The patients were diagnosed and classified as tAD and PCA on the basis of clinical presentation, so we cannot be certain about the neuropathology underlying the symptoms and the possibility that some patients may have coexistent, additional neuropathologies cannot be excluded. Finally, the cortical diffusivity measures presented here were not directly shown in this study to measure minicolumn organization or any specific cytoarchitectural component and may instead represent a surrogate of the combination of cortical changes occurring during neurodegeneration, most likely including minicolumn breakdown and other processes.

In conclusion, different diffusion values were found, predominantly in the posterior cortical and temporal regions, between PCA and tAD subjects. These novel diffusion values showed excellent discrimination power to differentiate between tAD and PCA. This study provides insights into the role that cortical diffusivity could play as a potential method for investigating the microstructural changes in diverse neuropathological conditions. Further studies with ex-vivo cohorts and larger in-vivo cohorts will be necessary to generalize these findings.