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Lasers in Medical Science
Erschienen in: Lasers in Medical Science 6/2019

02.01.2019 | Original Article

Could a chelant improve the effect of curcumin-mediated photodynamic antimicrobial chemotherapy against dental intact biofilms?

verfasst von: Daniela Alejandra Cusicanqui Méndez, Veridiana Lopes Rizzato, Giuliana Campos Chaves Lamarque, Evandro José Dionisio, Marília Afonso Rabelo Buzalaf, Daniela Rios, Maria Aparecida Andrade Moreira Machado, Thiago Cruvinel

Erschienen in: Lasers in Medical Science | Ausgabe 6/2019

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Abstract

To our knowledge, there is still no evidence in relation to the combination of curcumin with chelants to improve the effects of antimicrobial photodynamic therapy (aPDT) on complex dental caries biofilms. Therefore, the aim of this study was to evaluate the antimicrobial effect of curcumin-ethylenediaminetetraacetic acid (EDTA)-mediated aPDT on the vitality of intact biofilms of dentin caries microcosms. Biofilms were grown on glass slabs in McBain medium plus 1% sucrose in microaerophily at 37 °C for 5 days. Then, biofilms were treated with associations of 600 μmol L−1 curcumin combined or not with 1% EDTA and 37.5 or 75 J cm−2 LED (455 nm). The vitality was determined by a confocal laser scanning microscopy (CLSM) after staining biofilms with a mixture of 2.5 g L−1 fluorescein diacetate and 0.25 g L−1 ethidium bromide. Statistical analysis was conducted by Kruskal-Wallis and post hoc Dunn’s test (P < 0.05). Three treatments were able to reduce the vitality of overall biofilms: curcumin + 75 J cm−2 LED, curcumin-EDTA + 37.5 J cm−2 LED, and curcumin-EDTA + 75 J cm−2 LED. Also, the vitality of inner layers of biofilms was significantly reduced only after the combination of aPDT with EDTA. Therefore, the association of curcumin and EDTA improved the antimicrobial effect of aPDT on dentin caries microcosms, considering the application of lower light densities and deeper layers of biofilms.
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Metadaten
Titel
Could a chelant improve the effect of curcumin-mediated photodynamic antimicrobial chemotherapy against dental intact biofilms?
verfasst von
Daniela Alejandra Cusicanqui Méndez
Veridiana Lopes Rizzato
Giuliana Campos Chaves Lamarque
Evandro José Dionisio
Marília Afonso Rabelo Buzalaf
Daniela Rios
Maria Aparecida Andrade Moreira Machado
Thiago Cruvinel
Publikationsdatum
02.01.2019
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 6/2019
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-018-02708-x

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