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Journal of Assisted Reproduction and Genetics

Erschienen in:

11.05.2017 | Assisted Reproduction Technologies

Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS

verfasst von: S. Mateo, F. Vidal, M. Parriego, I. Rodríguez, V. Montalvo, A. Veiga, M. Boada

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 7/2017

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Abstract

Purpose

The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage.

Methods

Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012–December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator. Embryo biopsy was performed on day 3 and genetic analysis approached by array comparative genomic hybridization.

Results

Chromosomal analysis revealed that 17% (15/88) of embryos derived from 1PN 2PB zygotes were diagnosed as euploid. After blastomere biopsy at day 3, the blastocyst rate at day 5 was 3.4% (3/88). Only 2.3% (2/88) euploid blastocysts were obtained. In two couples and after counseling and patient agreement, the transfer of a euploid blastocyst from a 1PN 2PB ICSI zygote was performed resulting in the birth of a healthy child.

Conclusions

These results open the possibility to consider embryos coming from 1PN 2PB ICSI zygotes for transfer when no other embryos from 2PN 2PB ICSI zygotes are available and if a PGS diagnosis of euploidy is obtained. Confirmation of biparental inheritance is strongly recommended.

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Titel: Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS
verfasst von: S. Mateo
F. Vidal
M. Parriego
I. Rodríguez
V. Montalvo
A. Veiga
M. Boada
Publikationsdatum: 11.05.2017
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 7/2017
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-017-0937-z

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Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS

Abstract

Purpose

Methods

Results

Conclusions

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Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

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Stillende Frauen haben geringeres kardiovaskuläres Risiko

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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