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03.08.2020 | COVID-19 | Commentary Zur Zeit gratis

Cryopreservation and IVF in the time of Covid-19: what is the best good tissue practice (GTP)?

verfasst von: Kimball O. Pomeroy, Mitchel C. Schiewe

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 10/2020

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Abstract

Examine good tissue practices as relates to in vitro fertilization, biopsying, and vitrificationto compare current knowledge of ova, sperm, and embryos as vectors for disease transmission as it relates to our current knowledge regarding the SARS-CoV-2 virus.
Unknown risks relating to the SARS-CoV-2 virus and sperm, ova, and embryos necessitate a reexamining of how human IVF is performed. Over the last decade, improvements in cryosurvival and live birth outcomes have been associated with zona pellucida breaching procedures (e.g., blastocyst collapsing and biopsying). In turn, today embryos are generally no longer protected by an intact zona pellucida when vitrified and in cryostorage. Additionally, high security storage containers have proven to be resilient to potential cross-contamination and reliable for routine human sperm freezing and embryo vitrification.
Several options to current IVF practices are presented that can effectively mitigate the risks of cross-contamination and infection due to the current Covid-19 pandemic or other viral exposures. The question remains; is heightened security and change warranted where the risks of disease transmission likely remain negligible?
Literatur
1.
Zurück zum Zitat Pomeroy K, Harris S, Conaghan J, Papadakis M, Centola G, Basuray R, et al. Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk. Fertil Steril. 2010;94(4):1181–8.CrossRef Pomeroy K, Harris S, Conaghan J, Papadakis M, Centola G, Basuray R, et al. Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk. Fertil Steril. 2010;94(4):1181–8.CrossRef
5.
Zurück zum Zitat Prussin A II, Marr LC. Source of airborne microorganisms in the built environment. Microbiome. 2015;3:78–88.CrossRef Prussin A II, Marr LC. Source of airborne microorganisms in the built environment. Microbiome. 2015;3:78–88.CrossRef
6.
Zurück zum Zitat Qian J, Hospodsky D, Yamamoto N, Nazaroff W, Peccia J. Size-resolved emission rates of airborne bacteria and fungi in an occupied classroom. Indoor Air. 2012;22:339–51.CrossRef Qian J, Hospodsky D, Yamamoto N, Nazaroff W, Peccia J. Size-resolved emission rates of airborne bacteria and fungi in an occupied classroom. Indoor Air. 2012;22:339–51.CrossRef
7.
Zurück zum Zitat Bielanski A. A review of the risk of contamination of semen and embryos during cryopreservation and measures to limit cross-contamination during banking to prevent disease transmission in ET practices. Theriogenology. 2012;77(3):467–82.CrossRef Bielanski A. A review of the risk of contamination of semen and embryos during cryopreservation and measures to limit cross-contamination during banking to prevent disease transmission in ET practices. Theriogenology. 2012;77(3):467–82.CrossRef
9.
Zurück zum Zitat Hema N, Raj N, Chaithanya E, Chincholi R, Iswariya M, Hema K. J. Oral Maxi Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus among dental students: an in vivo study. J Oral Maxillofac Pathol. 2017;21(3):356–9.CrossRef Hema N, Raj N, Chaithanya E, Chincholi R, Iswariya M, Hema K. J. Oral Maxi Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus among dental students: an in vivo study. J Oral Maxillofac Pathol. 2017;21(3):356–9.CrossRef
12.
Zurück zum Zitat Holtmann N, Edimiris P, Andree M, Doehmen C, Baston-Buest D, Adams O, et al. Assessment of SARS-CoV-2 in human semen - a cohort study. Fertil Steril. In Press. Holtmann N, Edimiris P, Andree M, Doehmen C, Baston-Buest D, Adams O, et al. Assessment of SARS-CoV-2 in human semen - a cohort study. Fertil Steril. In Press.
13.
Zurück zum Zitat Pan F, Xiao X, Guo J, Song Y, Li H, Patel D, et al. No evidence of SARS-CoV-2 in 393 semen of males recovering from COVID-19. Fertil Steril. 2020;113(6):1135–9.CrossRef Pan F, Xiao X, Guo J, Song Y, Li H, Patel D, et al. No evidence of SARS-CoV-2 in 393 semen of males recovering from COVID-19. Fertil Steril. 2020;113(6):1135–9.CrossRef
14.
Zurück zum Zitat Araneta M, Mascola L, Eller A, O'Neil L, Ginsberg M, Bursaw M, et al. HIV transmission through donor artificial insemination. JAMA. 1995;273(11):854–8.CrossRef Araneta M, Mascola L, Eller A, O'Neil L, Ginsberg M, Bursaw M, et al. HIV transmission through donor artificial insemination. JAMA. 1995;273(11):854–8.CrossRef
15.
Zurück zum Zitat Quayle A, Zu C, Mayer K, Anderson DJ. T lymphocytes and macrophages, but not motile spermatozoa, are a significant source of human immunodeficiency virus in semen. J Infect Dis. 1997;176:960–8.CrossRef Quayle A, Zu C, Mayer K, Anderson DJ. T lymphocytes and macrophages, but not motile spermatozoa, are a significant source of human immunodeficiency virus in semen. J Infect Dis. 1997;176:960–8.CrossRef
16.
Zurück zum Zitat Loskutoff N, Huyser C, Singh R, Tech B, Walker D, Thornhill A, et al. Use of a novel washing method combining multiple density gradients and trypsin for removing human immunodeficiency virus-1 and hepatitis C virus from semen. Fertil Steril. 2005;84:1001–10.CrossRef Loskutoff N, Huyser C, Singh R, Tech B, Walker D, Thornhill A, et al. Use of a novel washing method combining multiple density gradients and trypsin for removing human immunodeficiency virus-1 and hepatitis C virus from semen. Fertil Steril. 2005;84:1001–10.CrossRef
17.
Zurück zum Zitat Dineen T, Woodward B. Other factors to consider with sperm preparation for treatment. In: Jayant Mehta BW, editor. Male infertility: sperm diagnosis, management and delivery. JP Medical Publishers, Ltd: London; 2014. p. 71–80. Dineen T, Woodward B. Other factors to consider with sperm preparation for treatment. In: Jayant Mehta BW, editor. Male infertility: sperm diagnosis, management and delivery. JP Medical Publishers, Ltd: London; 2014. p. 71–80.
18.
Zurück zum Zitat Givens D, Stringfellow DA. Manual of the International Embryo Transfer Society: a procedural guide and general information for the use of embryo transfer technology, emphasizing sanitary precautions. 4th ed. Champaign: International Embryo Transfer Society; 2008. p. 1–145. Givens D, Stringfellow DA. Manual of the International Embryo Transfer Society: a procedural guide and general information for the use of embryo transfer technology, emphasizing sanitary precautions. 4th ed. Champaign: International Embryo Transfer Society; 2008. p. 1–145.
20.
Zurück zum Zitat Parmegiani L, Accorsi A, Cognigni G, Bernardi S, Troilo E, Filicori M. Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos. Fertil Steril. 2010;94(4):1525–8.CrossRef Parmegiani L, Accorsi A, Cognigni G, Bernardi S, Troilo E, Filicori M. Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos. Fertil Steril. 2010;94(4):1525–8.CrossRef
21.
Zurück zum Zitat Parmegiani L, Accorsi A, Bernardi S, Arnone A, Cognigni G, Filicori M. A reliable procedure for decontamination before thawing of human specimens cryostored in liquid nitrogen: three washes with sterile liquid nitrogen (SLN2). Fertil Steril. 2012;98(4):870–5.CrossRef Parmegiani L, Accorsi A, Bernardi S, Arnone A, Cognigni G, Filicori M. A reliable procedure for decontamination before thawing of human specimens cryostored in liquid nitrogen: three washes with sterile liquid nitrogen (SLN2). Fertil Steril. 2012;98(4):870–5.CrossRef
22.
Zurück zum Zitat Bielanski A, Dubuc C. In vitro fertilization of ova from cows experimentally infected with a non-cytopathic strain of bovine viral diarrhea virus. Anim Reprod Sci. 1995;38:215–22.CrossRef Bielanski A, Dubuc C. In vitro fertilization of ova from cows experimentally infected with a non-cytopathic strain of bovine viral diarrhea virus. Anim Reprod Sci. 1995;38:215–22.CrossRef
23.
Zurück zum Zitat Cobo A, Bellver J, de los Santos M, Remohí J. Viral screening of spent culture media and liquid nitrogen samples of oocytes and embryos from hepatitis B, hepatitis C, and human immunodeficiency virus chronically infected women undergoing in vitro fertilization cycles. Fertil Steril. 2012;97(1):74–8.CrossRef Cobo A, Bellver J, de los Santos M, Remohí J. Viral screening of spent culture media and liquid nitrogen samples of oocytes and embryos from hepatitis B, hepatitis C, and human immunodeficiency virus chronically infected women undergoing in vitro fertilization cycles. Fertil Steril. 2012;97(1):74–8.CrossRef
24.
Zurück zum Zitat Tedder R, Zuckerman M, Goldstone A, Hawkins A, Fielding A, Briggs E, et al. Hepatitis B transmission from a contaminated cryopreservation tank. Lancet. 1995;346:137–40.CrossRef Tedder R, Zuckerman M, Goldstone A, Hawkins A, Fielding A, Briggs E, et al. Hepatitis B transmission from a contaminated cryopreservation tank. Lancet. 1995;346:137–40.CrossRef
25.
Zurück zum Zitat Bielanski A, Nadin-Davis S, Sapp T, Lutz-Wallace C. Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology. 2000;40(2):110–6.CrossRef Bielanski A, Nadin-Davis S, Sapp T, Lutz-Wallace C. Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology. 2000;40(2):110–6.CrossRef
26.
Zurück zum Zitat Kyuwa S, Nishikawa T, Kaneko T, Nakashima T, Kawano K, Nakamura N, et al. Experimental evaluation of cross-contamination between cryotubes containing mouse 2-cell embryos and murine pathogens in liquid nitrogen tanks. Exp Anim. 2003;52(1):67–70.CrossRef Kyuwa S, Nishikawa T, Kaneko T, Nakashima T, Kawano K, Nakamura N, et al. Experimental evaluation of cross-contamination between cryotubes containing mouse 2-cell embryos and murine pathogens in liquid nitrogen tanks. Exp Anim. 2003;52(1):67–70.CrossRef
27.
Zurück zum Zitat Van Landuyt L, Verpoest W, Verheyen G, De Vos A, Van de Velde H, Liebaers I, et al. Closed blastocyst vitrification of biopsied embryos: evaluation of 100 consecutive warming cycles. Hum Reprod. 2010;26:316–22.CrossRef Van Landuyt L, Verpoest W, Verheyen G, De Vos A, Van de Velde H, Liebaers I, et al. Closed blastocyst vitrification of biopsied embryos: evaluation of 100 consecutive warming cycles. Hum Reprod. 2010;26:316–22.CrossRef
28.
Zurück zum Zitat Schiewe MC, Zozula S, Anderson RE, Fahy GM. Validation of microSecure vitrification (μS-VTF) for the effective cryopreservation of human embryos and oocytes. Cryobiology. 2015;71:264–72.CrossRef Schiewe MC, Zozula S, Anderson RE, Fahy GM. Validation of microSecure vitrification (μS-VTF) for the effective cryopreservation of human embryos and oocytes. Cryobiology. 2015;71:264–72.CrossRef
29.
Zurück zum Zitat Schiewe MC, Zozula S, Nugent N, Waggoner K, Borba J, Gamboa L, et al. Modified microSecure vitrification: a safe, simple and highly effective cryopreservation procedure for human blastocysts. J Vis Exp. 2017;121:e54871. Schiewe MC, Zozula S, Nugent N, Waggoner K, Borba J, Gamboa L, et al. Modified microSecure vitrification: a safe, simple and highly effective cryopreservation procedure for human blastocysts. J Vis Exp. 2017;121:e54871.
30.
Zurück zum Zitat Panagiotidis Y, Vanderzwalmen P, Prapas Y, Kasapi E, Goudakou M, Papatheodorou A, et al. Open versus closed vitrification of blastocysts from an oocyte-donation programme: a prospective randomized study. Reprod BioMed Online. 2013;26:470–6.CrossRef Panagiotidis Y, Vanderzwalmen P, Prapas Y, Kasapi E, Goudakou M, Papatheodorou A, et al. Open versus closed vitrification of blastocysts from an oocyte-donation programme: a prospective randomized study. Reprod BioMed Online. 2013;26:470–6.CrossRef
31.
Zurück zum Zitat Papatheodorou A, Vanderzwalmen P, Panagiotidis Y, Prapas N, Zikopoulos K, Georgiou I, et al. Open versus closed oocyte vitrification system: a prospective randomized study. Reprod BioMed Online. 2013;26:595–602.CrossRef Papatheodorou A, Vanderzwalmen P, Panagiotidis Y, Prapas N, Zikopoulos K, Georgiou I, et al. Open versus closed oocyte vitrification system: a prospective randomized study. Reprod BioMed Online. 2013;26:595–602.CrossRef
32.
Zurück zum Zitat Wirleitner B, Vanderzwalmen P, Bach M, Baramsai B, Neyer A, Schwerda D, et al. The time aspect in storing vitrified blastocysts: its impact on survival rate, implantation potential and babies born. Hum Reprod. 2013;28:2950–7.CrossRef Wirleitner B, Vanderzwalmen P, Bach M, Baramsai B, Neyer A, Schwerda D, et al. The time aspect in storing vitrified blastocysts: its impact on survival rate, implantation potential and babies born. Hum Reprod. 2013;28:2950–7.CrossRef
33.
Zurück zum Zitat Mortimer D. Current and future concepts and practices in human sperm cryobanking. Reprod BioMed Online. 2004;9(2):134–51.CrossRef Mortimer D. Current and future concepts and practices in human sperm cryobanking. Reprod BioMed Online. 2004;9(2):134–51.CrossRef
34.
Zurück zum Zitat Anderson R, Whitney J, Schiewe M. Clinical benefits of preimplantation genetic testing for aneuploidy (PGT-A) for all in vitro fertilization treatment cycles. J Eur Med Genet. 2019;62:103731. Anderson R, Whitney J, Schiewe M. Clinical benefits of preimplantation genetic testing for aneuploidy (PGT-A) for all in vitro fertilization treatment cycles. J Eur Med Genet. 2019;62:103731.
35.
Zurück zum Zitat Practice Committee of the American Society for Reproductive Medicine and The Society for Assisted Reproductive Technology. The use of preimplantation genetic testing for aneuploidy (PGT-A: a committee opinion). Fertil Steril. 2018;109(3):429–36.CrossRef Practice Committee of the American Society for Reproductive Medicine and The Society for Assisted Reproductive Technology. The use of preimplantation genetic testing for aneuploidy (PGT-A: a committee opinion). Fertil Steril. 2018;109(3):429–36.CrossRef
Metadaten
Titel
Cryopreservation and IVF in the time of Covid-19: what is the best good tissue practice (GTP)?
verfasst von
Kimball O. Pomeroy
Mitchel C. Schiewe
Publikationsdatum
03.08.2020
Verlag
Springer US
Schlagwort
COVID-19
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 10/2020
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-020-01904-5

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