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Erschienen in: Maternal and Child Health Journal 9/2020

16.07.2020 | COVID-19 | From the Field Zur Zeit gratis

Using Prenatal Blood Samples to Evaluate COVID-19 Rapid Serologic Tests Specificity

verfasst von: Jackeline Alger, Maria Luisa Cafferata, Tito Alvarado, Alvaro Ciganda, Arturo Corrales, Hans Desale, Arnaud Drouin, Dahlene Fusco, Jorge Garcia, Luz Gibbons, Emily Harville, Wendy Lopez, Ivette Lorenzana, Fausto Muñoz-Lara, Elsa Palou, Eduardo Retes, Manuel Sierra, Candela Stella, Xu Xiong, Lysien I. Zambrano, Pierre Buekens

Erschienen in: Maternal and Child Health Journal | Ausgabe 9/2020

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Abstract

Introduction

Background cross-reactivity with other coronaviruses may reduce the specificity of COVID-19 rapid serologic tests. The vast majority of women attend prenatal care, which is a unique source of population-based blood samples appropriate for validation studies. We used stored 2018 serum samples from an existing pregnancy cohort study to evaluate the specificity of COVID-19 serologic rapid diagnostic tests.

Methods

We randomly selected 120 stored serum samples from pregnant women enrolled in a cohort in 2018 in Tegucigalpa, Honduras, at least 1 year before the COVID-19 pandemic. We used stored serum to evaluate four lateral flow rapid diagnostic tests, following manufacturers’ instructions. Pictures were taken for all tests and read by two blinded trained evaluators.

Results

We evaluated 120, 80, 90, and 90 samples, respectively. Specificity for both IgM and IgG was 100% for the first two tests (95% confidence intervals [CI] 97.0–100 and 95.5–100, respectively). The third test had a specificity of 98.9% (95% CI 94.0–100) for IgM and 94.4% (95% CI 87.5–98.2) for IgG. The fourth test had a specificity of 88.9% (95% CI 80.5–94.5) for IgM and 100% (95% CI 96.0–100) for IgG.

Discussion

COVID-19 serologic rapid tests are of variable specificity. Blood specimens from sentinel prenatal clinics provide an opportunity to validate serologic tests with population-based samples.
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Metadaten
Titel
Using Prenatal Blood Samples to Evaluate COVID-19 Rapid Serologic Tests Specificity
verfasst von
Jackeline Alger
Maria Luisa Cafferata
Tito Alvarado
Alvaro Ciganda
Arturo Corrales
Hans Desale
Arnaud Drouin
Dahlene Fusco
Jorge Garcia
Luz Gibbons
Emily Harville
Wendy Lopez
Ivette Lorenzana
Fausto Muñoz-Lara
Elsa Palou
Eduardo Retes
Manuel Sierra
Candela Stella
Xu Xiong
Lysien I. Zambrano
Pierre Buekens
Publikationsdatum
16.07.2020
Verlag
Springer US
Schlagwort
COVID-19
Erschienen in
Maternal and Child Health Journal / Ausgabe 9/2020
Print ISSN: 1092-7875
Elektronische ISSN: 1573-6628
DOI
https://doi.org/10.1007/s10995-020-02981-9

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