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Erschienen in: Critical Care 1/2020

Open Access 01.12.2020 | COVID-19 | Research Letter

Weak anti-SARS-CoV-2 antibody response is associated with mortality in a Swedish cohort of COVID-19 patients in critical care

verfasst von: Sana Asif, Robert Frithiof, Miklos Lipcsey, Bjarne Kristensen, Kjell Alving, Michael Hultström

Erschienen in: Critical Care | Ausgabe 1/2020

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To the editor,
Profiling of the antibody responses to SARS-CoV-2 may be crucial to understand the immunological reaction and to design successful treatment strategies. Studies have confirmed the development of a typical antibody response to an acute viral infection in COVID-19 patients [1]. A robust generation and a dynamic pattern of IgA, IgM and IgG antibodies can be detected 2–3 weeks following the first symptoms of COVID-19 [2, 3]. An early robust antibody response in patients hospitalized with severe COVID-19 was reported in survivors, versus a weak antibody production in non-survivors [4, 5]. However, antibody responses to SARS-CoV-2 in critically ill patients is largely unknown.
We investigated the antibody response to SARS-Cov-2 Spike-1 protein in adult patients (n = 19) admitted to the intensive care unit (ICU) at a tertiary care hospital in Uppsala, Sweden. Plasma samples were collected at two different time points; (a) early, day 0–3 and (b) late, day 10–13, and concentrations of IgA, IgG and IgM antibodies were quantified by FluoroEnzymeImmunoassay (FEIA), Phadia AB, Uppsala, Sweden.
The median age of our cohort was 57 years, and 93% were males. The most common co-morbidities were obesity (93%), hypertension (42%) and diabetes mellitus type-2 (32%). The median COVID-19 day at ICU admission was 10 (6–14) days, the median length of ICU stay was 18 (11–38) days, and 30 days mortality was 21% (Table 1). In our cohort, a IgA, IgG and IgM antibody response could be detected as early as day 0–3 post-ICU admission, and this increase in antibodies was persistent up to day 10–13 (Fig. 1). A significant change in antibody concentrations over time was detected in patients who survived till day 30 in comparison with those who did not (Fig. 1). No associations were seen between antibody levels and patient age, or any other clinical or laboratory parameters. At both early and late timepoints, plasma concentrations of IgA, IgG and IgM antibodies tend to be higher in patients who survived compared to those who had died at 30 days (Fig. 1). This suggests that SARS-CoV-2 antibody response, similar to other virus illnesses, is important for virus protection and recovery. A limitation of the present dataset is the relatively low number of patients.
Table 1
Clinical characteristics of COVID-19 patients (n = 19) with at least 10 days length-of-stay in intensive care unit (ICU)
Variables
 
Cohort characteristics
Median (range)
 Age, years, median (range)
57 (26–76)
 Male, n (%)
18 (93)
 COVID-19 days, median (range)
10 (6–14)
 Length-of-stay in ICU, median (range)
18 (11–38)
 30-day mortality, n (%)
4 (21)
Comorbidities
n (%)
 Obesity (BMI > 25)
18 (93)
 Diabetes mellitus
6 (32)
 Hypertension
8 (42)
 Pulmonary disease
4 (21)
 Cardiovascular disease
3 (16)
Clinical parameters
Median (range)
 Fever (> 38 °C), n (%)
15 (79)
 Mean arterial pressure, mmHg, median (range)
94 (68–137)
 Heart rate, min−1, median (range)
92 (67–116)
 Respiratory rate, min−1, median (range)
31 (15–50)
 SAPS3 score, median (range)
49 (39–63)
Laboratory parameters
 SOFA score
7 (3–9)
 CRP mg/L
241 (131–476)
 Ferritin μg/L
676 (104–3960)
 Lactate mmol/L
1.1 (0.8–1.7)
SAPS3 simplified acute physiology score-3, SOFA score sequential organ failure assessment score, CRP C-reactive protein
To our knowledge, this study provides the earliest evidence that an early and potent antibody response may contribute to infection clearance and improved prognosis in patients critically ill with COVID-19.

Acknowledgements

The authors thank research nurses Joanna Wessbergh and Elin Söderman, and the biobank assistants Erik Danielsson and Philip Karlsson for their expertise in compiling the study.
The presented data are part of a study approved by the National Ethical Review Agency (EPM; No. 2020-01623). Informed consent was obtained from the patient, or next of kin if the patient was unable to give consent. The Declaration of Helsinki and its subsequent revisions were followed.
Not applicable.

Competing interests

The authors declare that they have no conflict of interest.
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://​creativecommons.​org/​licenses/​by/​4.​0/​. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Metadaten
Titel
Weak anti-SARS-CoV-2 antibody response is associated with mortality in a Swedish cohort of COVID-19 patients in critical care
verfasst von
Sana Asif
Robert Frithiof
Miklos Lipcsey
Bjarne Kristensen
Kjell Alving
Michael Hultström
Publikationsdatum
01.12.2020
Verlag
BioMed Central
Schlagwort
COVID-19
Erschienen in
Critical Care / Ausgabe 1/2020
Elektronische ISSN: 1364-8535
DOI
https://doi.org/10.1186/s13054-020-03362-y

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