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01.12.2014 | Research | Ausgabe 1/2014 Open Access

Chinese Medicine 1/2014

Cratoxylum formosum (Jack) Dyer ssp. pruniflorum (Kurz) Gogel. (Hóng yá mù) extract induces apoptosis in human hepatocellular carcinoma HepG2 cells through caspase-dependent pathways

Zeitschrift:
Chinese Medicine > Ausgabe 1/2014
Autoren:
Apiyada Nonpunya, Natthida Weerapreeyakul, Sahapat Barusrux
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1749-8546-9-12) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no conflict of interests.

Authors’ contributions

NW conceived and designed the study. AN performed the experiments and statistical analysis. NW and AN wrote the manuscript. SB reviewed the literature, revised the manuscript and coordinated the study. All the authors read and approved the final version of the manuscript.

Abstract

Background

Cratoxylum formosum (Jack) Dyer ssp. pruniflorum (Kurz) Gogel. (Hóng yá mù) (CF) has been used for treatment of fever, cough, and peptic ulcer. Previously, a 50% ethanol-water extract from twigs of CF was shown highly selective in cytotoxicity against cancer cells. This study aims to investigate the molecular mechanisms underlying the apoptosis-inducing effect of CF.

Methods

The cytotoxicity of CF was evaluated in the human hepatocellular carcinoma (HCC) HepG2 cell line in comparison with a non-cancerous African green monkey kidney epithelial cell line (Vero) by a neutral red assay. The apoptosis induction mechanisms were investigated through nuclear morphological changes, DNA fragmentation, mitochondrial membrane potential alterations, and caspase enzyme activities.

Results

CF selectively induced HepG2 cell death compared with non-cancerous Vero cells. A 1.5-fold higher apoptotic effect compared with melphalan was induced by 120 μg/mL of the 50% ethanol-water extract of CF. The apoptotic cell death in HepG2 cells occurred via extrinsic and intrinsic caspase-dependent pathways in dose- and time-dependent manners by significantly increasing the activities of caspase 3/7, 8, and 9, decreasing the mitochondrial membrane potential, and causing apoptotic body formation and DNA fragmentation.

Conclusions

CF extract induced a caspase-dependent apoptosis in HepG2 cells.
Zusatzmaterial
Authors’ original file for figure 1
13020_2013_207_MOESM1_ESM.pdf
Authors’ original file for figure 2
13020_2013_207_MOESM2_ESM.pdf
Authors’ original file for figure 3
13020_2013_207_MOESM3_ESM.pdf
Authors’ original file for figure 4
13020_2013_207_MOESM4_ESM.pdf
Authors’ original file for figure 5
13020_2013_207_MOESM5_ESM.pdf
Literatur
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