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Erschienen in: European Journal of Nutrition 2/2020

26.02.2019 | Original Contribution

Creatine supplementation in Walker-256 tumor-bearing rats prevents skeletal muscle atrophy by attenuating systemic inflammation and protein degradation signaling

verfasst von: Paola S. Cella, Poliana C. Marinello, Fernando H. Borges, Diogo F. Ribeiro, Patrícia Chimin, Mayra T. J. Testa, Philippe B. Guirro, José A. Duarte, Rubens Cecchini, Flávia A. Guarnier, Rafael Deminice

Erschienen in: European Journal of Nutrition | Ausgabe 2/2020

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Abstract

Purpose

The aim of this study was to investigate the effects of creatine supplementation on muscle wasting in Walker-256 tumor-bearing rats.

Methods

Wistar rats were randomly assigned into three groups (n = 10/group): control (C), tumor bearing (T), and tumor bearing supplemented with creatine (TCr). Creatine was provided in drinking water for a total of 21 days. After 11 days of supplementation, tumor cells were implanted subcutaneously into T and TCr groups. The animals’ weight, food and water intake were evaluated along the experimental protocol. After 10 days of tumor implantation (21 total), animals were euthanized for inflammatory state and skeletal muscle cross-sectional area measurements. Skeletal muscle components of ubiquitin–proteasome pathways were also evaluated using real-time PCR and immunoblotting.

Results

The results showed that creatine supplementation protected tumor-bearing rats against body weight loss and skeletal muscle atrophy. Creatine intake promoted lower levels of plasma TNF-α and IL-6 and smaller spleen morphology changes such as reduced size of white pulp and lymphoid follicle compared to tumor-bearing rats. In addition, creatine prevented increased levels of skeletal muscle Atrogin-1 and MuRF-1, key regulators of muscle atrophy.

Conclusion

Creatine supplementation prevents skeletal muscle atrophy by attenuating tumor-induced pro-inflammatory environment, a condition that minimizes Atrogin-1 and MuRF-1-dependent proteolysis.
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Metadaten
Titel
Creatine supplementation in Walker-256 tumor-bearing rats prevents skeletal muscle atrophy by attenuating systemic inflammation and protein degradation signaling
verfasst von
Paola S. Cella
Poliana C. Marinello
Fernando H. Borges
Diogo F. Ribeiro
Patrícia Chimin
Mayra T. J. Testa
Philippe B. Guirro
José A. Duarte
Rubens Cecchini
Flávia A. Guarnier
Rafael Deminice
Publikationsdatum
26.02.2019
Verlag
Springer Berlin Heidelberg
Erschienen in
European Journal of Nutrition / Ausgabe 2/2020
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-019-01933-6

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