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01.09.2018 | Brief Report

Cryo-EM structure reveals cylindrical nucleocapsids from two polydnaviruses

verfasst von: Ji-Hui Cui, Ya-Bin Chen, Ming Li, Qiu-Chen Cai, Li-Dan Zhang, Zi-Yun Lu, Jian-Cheng Li, Qi-Shun Zhu, Gang Ji, Kai-Jun Luo

Erschienen in: Archives of Virology | Ausgabe 12/2018

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Abstract

Bracovirus is one of the two polydnavirus genera. Here, we used a cryo-EM analysis to reveal the near-native morphology of two nucleocapsid-containing model bracoviruses: Microplitis bicoloratus bracovirus (MbBV) and Microplitis mediator bracovirus (MmBV). MbBV and MmBV nucleocapsids have discernable cap structures in two distal regions with relatively high electron density. Adjacent to the end-cap structures are two electron-lucent rings. Some nucleocapsids were uniformly electron-dense and had a distinctive “helix-tail-like structure”. Cryo-EM revealed inconsistent nucleocapsid diameters of 34–69.9 nm in MbBV and 46–69.9 nm in MmBV, and the largest observed cylindrical area length was expanded to 126 nm.

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Titel: Cryo-EM structure reveals cylindrical nucleocapsids from two polydnaviruses
verfasst von: Ji-Hui Cui
Ya-Bin Chen
Ming Li
Qiu-Chen Cai
Li-Dan Zhang
Zi-Yun Lu
Jian-Cheng Li
Qi-Shun Zhu
Gang Ji
Kai-Jun Luo
Publikationsdatum: 01.09.2018
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 12/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3958-8

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