Skip to main content
main-content

04.02.2017 | Original Article | Ausgabe 3/2017

Comparative Clinical Pathology 3/2017

Cytotoxic activity of fumonisin B1 in Vero cells: comparison between 2D and 3D structural microplates

Zeitschrift:
Comparative Clinical Pathology > Ausgabe 3/2017
Autoren:
Doha Yahia, Yoichi Kamata

Abstract

The present study aimed to estimate cytotoxic activity of fumonisin B1 (FB1) in Vero cells using crystal violet staining assay (CVA) and lactate dehydrogenase assay (LDH) performed in flattened (2D) and three dimensional (3D) bottom (V, U, and M) microplates. Vero cells were exposed to different concentrations of FB1 ranging from 0 to 10 μg/ml for 24 and 48 h. Tween 20 was used as a positive control and acetonitrile 50% in water was used as a negative control. Results revealed that CVA could be performed in the F type microplate for 24 and 48 h but it was useless in the 3D microplates. LDH assay showed that FB1 induced significant cytotoxicity on Vero cells after 24 h exposure in all microplates. Using 3D bottom microplates allowed formation of spheroid cell aggregates and reduced cell adhesion. LDH release in the U type microplate showed a significant dose-dependent increase, while V type and M type microplates showed a significant dose-independent increase in all exposed cells. Forty-eight hours exposure to FB1 in 2D microplate induced time-related cytotoxicity in all exposed cells pronounced as increased percentage of dead cells and significant increase in LDH release at 0.01, 0.1, 1, and 10 μg/ml. It was concluded that 2D culture plate is suitable for both LDH and CVA while 3D microplates are suitable only for the LDH assay. LDH assay could detect the cytotoxic effect of FB1 at lower concentrations than crystal violet staining assay.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf SpringerMedizin.de. Zusätzlich können Sie eine Zeitschrift Ihrer Wahl in gedruckter Form beziehen – ohne Aufpreis.

Jetzt e.Med bestellen und 100 € sparen!

Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 3/2017

Comparative Clinical Pathology 3/2017 Zur Ausgabe

Neu im Fachgebiet Pathologie

01.02.2020 | Forensische Begutachtung | CME | Ausgabe 1/2020

Diagnose einer gewaltsamen Erstickung

Teil 1: Reevaluation der Spezifität makroskopischer und histomorphologischer Befunde

27.01.2020 | Schwerpunkt: Lunge | Ausgabe 1/2020

Immunhistochemiebasierte prädiktive Biomarker bei Lungenkarzinomen

27.01.2020 | Schwerpunkt: Lunge | Ausgabe 1/2020

Zytologie der primären Lungenkarzinome