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Indian Journal of Otolaryngology and Head & Neck Surgery

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22.01.2021 | Original Article

Cytotoxic Role of Chlorogenic Acid on Oral Squamous Cell Carcinoma Cell Line

verfasst von: Gitika Sharma, Mala Kamboj, Anjali Narwal, Rashmi Bhardwaj, Pooja Yadav

Erschienen in: Indian Journal of Otolaryngology and Head & Neck Surgery | Sonderheft 3/2022

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Abstract

The aim of the present study was to determine the cytotoxic, anticancerous and antiproliferative activity of CGA on oral squamous cell carcinoma (OSCC) cell line (KB) and to evaluate expression level of p21 and p53 in these CGA treated OSCC cell line. Different concentrations of CGA varying from 500 to 2500 µM were tested on OSCC cell line. Trypan blue and MTT assay were performed to establish IC₅₀. DNA fragmentation and expression level of p21 and p53 were evaluated with the help of RT-PCR. CGA exerted antiproliferative and cytotoxic effect on OSCC (KB) cell line. Statistically significant results were found regarding effect of different CGA concentrations on KB cell line with IC₅₀ at 1800 µM. No DNA fragmentation was observed. p21 and p53 expression were down regulated after CGA treatment. CGA revealed neither apoptosis nor damage to the nucleus after DNA fragmentation. Antiproliferative role of CGA was hinted by down regulation of p53 and p21 probably through cell cycle arrest at G₁-S phase. It was reaffirmed that CGA a natural chemo preventive agent could enhance the treatment modalities with minimal side effects.

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Titel: Cytotoxic Role of Chlorogenic Acid on Oral Squamous Cell Carcinoma Cell Line
verfasst von: Gitika Sharma
Mala Kamboj
Anjali Narwal
Rashmi Bhardwaj
Pooja Yadav
Publikationsdatum: 22.01.2021
Verlag: Springer India
Erschienen in: Indian Journal of Otolaryngology and Head & Neck Surgery / Ausgabe Sonderheft 3/2022
Print ISSN: 2231-3796
Elektronische ISSN: 0973-7707
DOI: https://doi.org/10.1007/s12070-021-02395-1

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Springer Medizin

Cytotoxic Role of Chlorogenic Acid on Oral Squamous Cell Carcinoma Cell Line

Abstract

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