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14.09.2016 | Original Paper | Ausgabe 1/2017 Open Access

Journal of Natural Medicines 1/2017

De novo transcriptome assembly and characterization of nine tissues of Lonicera japonica to identify potential candidate genes involved in chlorogenic acid, luteolosides, and secoiridoid biosynthesis pathways

Zeitschrift:
Journal of Natural Medicines > Ausgabe 1/2017
Autoren:
Amit Rai, Hidetaka Kamochi, Hideyuki Suzuki, Michimi Nakamura, Hiroki Takahashi, Tomoki Hatada, Kazuki Saito, Mami Yamazaki
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s11418-016-1041-x) contains supplementary material, which is available to authorized users.
We would like to dedicate this article to Professor Satoshi Ōmura and Professor William C. Campbell ‘for their discoveries concerning a novel therapy against infections caused by roundworm parasites’, and to Professor Youyou Tu ‘for her discoveries concerning a novel therapy against Malaria’. These discoveries had a great impact on human society, and they were acknowledged by being awarded the Nobel Prize in Physiology or Medicine in 2015.

Abstract

Lonicera japonica is one of the most important medicinal plants with applications in traditional Chinese and Japanese medicine for thousands of years. Extensive studies on the constituents of L. japonica extracts have revealed an accumulation of pharmaceutically active metabolite classes, such as chlorogenic acid, luteolin and other flavonoids, and secoiridoids, which impart characteristic medicinal properties. Despite being a rich source of pharmaceutically active metabolites, little is known about the biosynthetic enzymes involved, and their expression profile across different tissues of L. japonica. In this study, we performed de novo transcriptome assembly for L. japonica, representing transcripts from nine different tissues. A total of 22 Gbps clean RNA-seq reads from nine tissues of L. japonica were used, resulting in 243,185 unigenes, with 99,938 unigenes annotated based on a homology search using blastx against the NCBI-nr protein database. Unsupervised principal component analysis and correlation studies using transcript expression data from all nine tissues of L. japonica showed relationships between tissues, explaining their association at different developmental stages. Homologs for all genes associated with chlorogenic acid, luteolin, and secoiridoid biosynthesis pathways were identified in the L. japonica transcriptome assembly. Expression of unigenes associated with chlorogenic acid was enriched in stems and leaf-2, unigenes from luteolin were enriched in stems and flowers, while unigenes from secoiridoid metabolic pathways were enriched in leaf-1 and shoot apex. Our results showed that different tissues of L. japonica are enriched with sets of unigenes associated with specific pharmaceutically important metabolic pathways and, therefore, possess unique medicinal properties. The present study will serve as a resource for future attempts for functional characterization of enzyme coding genes within key metabolic processes.

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