Background
The World Health Organization (WHO) defines diarrhoea as “the passage of three or more loose or liquid stools per day, or more frequently than is normal for the individual” [
1]. Among travel related morbidity, traveller’s diarrhoea (TD) is in first place [
2]. The causative agents of TD are manifold (e.g. virus, bacteria, parasites) and often remain unidentified in culture-based routine microbiological analysis. Recently, various culture-independent syndromic multiplex assays were developed for the laboratory detection of a broad range of TD pathogens. Although these tests are more sensitive, their interpretation can become a challenge, if numerous pathogens are detected [
3]. In addition, some pathogens have a weak association with disease or low attributable fractions among TD cases (e.g.
Aeromonas sp.,
Plesiomonas shigelloides) [
4,
5]. Finally, the presence of amplifiable DNA does not always correlate with the presence of viable pathogens.
As molecular tests for gastrointestinal infections become more and more part of routine diagnostics [
6], there is a need to assess their clinical value for the diagnosis of TD. Therefore, the objective of this study was to evaluate the GI-EB Screening assay (Allplex™, Seegene, Düsseldorf, Germany) in a case-control study.
Results
A detailed description of the study population (
n = 91), the cases (
n = 61) and controls (
n = 30) is published elsewhere [
5]. Briefly, the TD cases were younger (mean age 24 vs. 39,
p < 0.001) and more likely female (66 vs. 33%,
p = 0.004) than controls. The majority of participants travelled to Africa (36%), Asia (34%) and North America (15%). The travel destinations were unbalanced between cases and controls as more cases travelled to Africa (OR = 2.5, 95%CI: 0.9–5,
p = 0.07) while a travel destination in South America was overrepresented in the control group (OR = 5, 95%CI: 1.4–25,
p = 0.01).
The average duration of signs and symptoms of TD was 4 days. Stool samples were stored at ambient temperature during travel and at − 20 °C after travel until analysis (storage time at − 20° was approx. 2 years).
In total, 29% of participants (26/91) were tested positive in the GI-EB Screening assay. The majority had only one pathogen (20/91), followed by two pathogens (4/91) and three or four pathogens (each 1/91).
Overall,
E. coli O157 was predominant (13/91), followed by STEC (9/91),
Shigella spp./EIEC (5/91),
Y. enterocolitica (5/91) and
Salmonella spp. (3/91). Noteworthy, three travellers (2 cases, 1 control) had a co-detection of
E. coli O157/STEC. Although
Salmonella spp. was only detected in cases, none of the pathogens included in the GI-EB Screening assay was significantly associated with TD cases. All AFs were 0–3.5 (Table
1).
Table 1
Evaluation of the GI-EB Screening assay (Seegene) in travellers with and without traveller’s diarrhoea (TD)
Escherichia coli O157 | 13 (14.3%) | 8 (13%) | 5 (17%) | 0.8 (0.2–2.5) | 0.65 | 0 (0–7.8) |
Shiga-toxin producing E. coli | 9 (9.9%) | 4 (7%) | 5 (17%) | 0.4 (0.1–1.4) | 0.15 | 0 (0–2.0) |
Shigella spp./Enteroinvasive E. coli | 5 (5.5%) | 4 (7%) | 1 (3%) | 2 (0.2–10) | 1 | 3.5 (0–6.3) |
Yersinia enterocolitica | 5 (5.5%) | 3 (5%) | 2 (7%) | 0.7 (0.1–5) | 1 | 0 (0–4) |
Salmonella spp. | 3 (3.3%) | 3 (5%) | 0 (0%) | 0 (0–NaN) | 0.55 | NA |
Clostridioides difficile toxin A/B | 0 (0%) | 0 (0%) | 0 (0%) | NA | NA | NA |
Campylobacter spp. | 0 (0%) | 0 (0%) | 0 (0%) | NA | NA | NA |
A higher pathogen load corresponds to a lower Ct-value (threshold cycle, i.e. number of cycles required for a positive result) and could be used to distinguish between asymptomatic colonization and TD (e.g. ETEC,
Campylobacter) assuming that pathogen concentration is higher in cases compared to controls [
4]. The mean Ct-values were significantly higher in cases compared to controls for
E. coli O157 (37.7 vs. 32.9,
p = 0.03). Cases and controls had comparable Ct-values for
Y. enterocolitica (34.3 vs. 33.6,
p = 0.9) and STEC (35.6 vs. 34.9,
p = 0.8). No comparison of Ct-values was done for
Salmonella spp. and
Shigella spp./EIEC as none or only one participant, respectively, was detected in the control group (Table
1).
The samples were also analysed by the BioFire® FilmArray® Gastrointestinal Panel for comparison [
5] and the majority of pathogens were enteropathogenic
E. coli (36/91, Table S
1).
The concordance between the GI-EB Screening assay and BioFire® FilmArray® Gastrointestinal Panel was 86.8–100% depending on the target pathogen (Table
2).
Table 2
Concordance between GI-EB Screening assay (Seegene) and BioFire® FilmArray® Gastrointestinal Panel (bioMérieux [
5])
BioFire® FilmArray® Gastrointestinal Panel | Escherichia coli O157 | Not detected | 76 | 6 | 91.2% (83/91) |
Detected | 2 | 7 |
Shiga-toxin producing E. coli | Not detected | 70 | 0 | 86.8% (79/91) |
Detected | 12 | 9 |
Shigella spp./Enteroinvasive E. coli | Not detected | 85 | 1 | 97.8% (89/91) |
Detected | 1 | 4 |
Yersinia enterocolitica | Not detected | 86 | 3 | 94.5% (88/91) |
Detected | 0 | 2 |
Salmonella spp. | Not detected | 88 | 0 | 100% (91/91) |
Detected | 0 | 3 |
Total costs for one analysis using the BioFire® FilmArray® Gastrointestinal Panel were 118.15 € (work force costs for 10 min: 3.15 €; consumable costs: 115.00€). One test with the GI-EB Screening assay costs 25.43 € (work force costs for 20 min: 6.30 €; consumable costs [incl. DNA extraction]: 19.13 €).
Discussion
We tested a selection of TD stool samples with the GI-EB Screening assay and found a low proportion of positive samples and a weak association with TD (Table
1). This weak test performance is most likely due to unsuitable target organisms of the test for the diagnosis of TD (Table
1). In general, ETEC (7–45%), enteropathogenic
E. coli (EPEC, 26–47%) and enteroaggregative
E. coli (EAEC, 5–46%) are predominant in TD in many countries [
2,
8].
Ct-values were significantly higher in cases compared to controls for
E. coli O157 suggesting a higher pathogen load in controls. The reason for this finding is, however, unclear and in contradiction with a larger study showing that the association with TD increases with lower Ct-values for STEC [
4].
The good concordance (86.8–100%) of the GI-EB Screening assay with BioFire® FilmArray® Gastrointestinal Panel is in line with another report that showed a concordance of 82.6–100% for bacterial pathogens between GI-EB Screening assay and other multiplex platforms that are currently used (e.g. BioFire®, Luminex xTAG®) [
9].
Syndromic multiplex tests are currently very much in vogue but their clinical value is often blurred due to the lack of asymptomatic control groups in the majority of studies [
10,
11]. Without this control group, one cannot calculate the AF, which is a benchmark in the evaluation of these multiplex tests as it “indicates the proportion of cases that can be attributed to a particular pathogen” [
2,
4]. Increasing detection rates or sensitivity does not imply an additional clinical value [
11]. We therefore suggest that future studies must include an asymptomatic control group to shed light on the question, which detected pathogen is truly of clinical relevance.
The total costs (personnel and reagents) were markedly lower for the GI-EB Screening assay (25.43 €) than the BioFire® FilmArray® Gastrointestinal Panel (118.15 €) and comparable to other commercial PCR-kits covering similar bacterial species (e.g. RIDA®GENE: 20–25 € without DNA extraction) [
12]. The more affordable PCR-kits, however, require a more sophisticated infrastructure (e.g. DNA extraction, test-platforms) and are suitable for processes in laboratories. In contrast, the more expensive BioFire® FilmArray® Gastrointestinal Panel has its strength as a point-of-care diagnostics.
Our study has limitations: First, the small sample size that does not allow for any conclusions on an association between the detection of rare pathogens and TD (e.g. Salmonella, Campylobacter). Second, we were unable to compare the multiplex-approaches with classical culture-based microbiological analyses, which was not done right after the return of the travellers. A post-hoc culture for enteropathogens more than two years after collection would lead to an unacceptably high rate of false-negative results by culture, particularly for fastidious pathogens such as Campylobacter sp. Third, we initially tested if sufficient controls are in our dataset to match them with the travel region of TD cases to rule out geographic confounders. Since we were unable to include matched controls for each case, we decided to select controls randomly. Therefore, the absence of an association between the detection of pathogens and TD might be confounded.
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