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Erschienen in: Lasers in Medical Science 8/2021

29.06.2021 | Review Article

An improved in vitro photochemical internalization protocol for 3D spheroid cultures

verfasst von: Lina Nguyen, Steen J. Madsen, Kristian Berg, Henry Hirschberg

Erschienen in: Lasers in Medical Science | Ausgabe 8/2021

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Abstract

Photochemical internalization (PCI) is a modified form of photodynamic therapy (PDT) that enhances the efficacy of therapeutic agents in a site and temporal specific manner in both in vitro and in vivo publications. The purpose of the study reported here was to evaluate the benefits of a modified PCI protocol in a 3D rat glioma spheroid model. In the modified protocol, F98 glioma cells were incubated with photosensitizer (AlPcS2a) prior to spheroid generation, as opposed to post-spheroid formation photosensitizer exposure commonly used in conventional protocols. The efficacy of both bleomycin and doxorubicin PCI was evaluated using either the conventional or modified protocols. The formed spheroids were then exposed to light treatment from a diode laser,  λ= 670 nm. Spheroid growth was monitored for a period of 14 days. The results of spheroid growth assays showed that there was no statistically significant difference in PCI efficacy between the conventional and modified protocols for both of the drugs tested. The direct PDT effect was significantly reduced using the modified protocol. Therefore, due to its several advantages, the modified protocol is recommended for evaluating the efficacy of PCI in tumor spheroid models.
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Metadaten
Titel
An improved in vitro photochemical internalization protocol for 3D spheroid cultures
verfasst von
Lina Nguyen
Steen J. Madsen
Kristian Berg
Henry Hirschberg
Publikationsdatum
29.06.2021
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 8/2021
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-021-03332-y