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Erschienen in:
01.11.2011 | original article
Cells from iliac crest and alveolar bone are very similar in their response to growth factors
Erschienen in: international journal of stomatology & occlusion medicine | Ausgabe 3/2011
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Background
Autologous bone from the iliac crest and alveolar bone are used to treat bony defects. New treatment options take advantage of growth factors and differentiation factors. It is however unknown whether bone cells from different sources respond in a similar way not only qualitatively but also quantitatively. In this study the responsiveness of cells isolated from both sources to growth factors in vitro was investigated.
Methods
Bone grafts were harvested from adult pigs and cells were isolated by enzymatic digestion. Cells were exposed to natural and recombinant growth factors. Proliferation and protein synthesis were determined by the incorporation of tritium-labelled thymidine and proline, respectively. Alkaline phosphatase activity served as a marker of osteogenic differentiation.
Results
Iliac crest and alveolar bone-derived cells showed similar increases in proliferation and protein synthesis in response to platelet-released supernatant and platelet-derived growth factor BB. The responsiveness to bone morphogenetic protein-6 was, however, more pronounced in cells obtained from the iliac crest than in those obtained from alveolar bone.
Conclusions
These findings suggest that cells from the iliac crest and alveolar bone are very similar in their response to growth factors which are present during graft consolidation.