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Erschienen in: International Journal of Hematology 4/2024

16.02.2024 | Original Article

Correlation of ex vivo and in vivo ammonia production with L-asparaginase biological activity in adults with lymphoid malignancies

verfasst von: Takayuki Nakamura, Shuki Oya, Hidetoshi Ozawa, Yoshimi Maehiro, Seiya Muta, Masahiro Umeda, Yusuke Takaki, Toshinobu Fukuyama, Yoshitaka Yamasaki, Maki Yamaguchi, Kazutoshi Aoyama, Fumihiko Mouri, Yoshiki Naito, Koji Nagafuji

Erschienen in: International Journal of Hematology | Ausgabe 4/2024

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Abstract

Silent inactivation of L-asparaginase (L-Asp) represents rapid clearance of L-Asp by anti-L-Asp IgG antibodies without clinical symptoms. Measurement of L-Asp activity is the gold standard for diagnosis of silent inactivation, but this test is not commercially available in Japan as of 2023. We evaluated ex vivo and in vivo ammonia production in relation to L-Asp activity. Blood samples from ten adult patients treated with L-Asp were collected to measure ammonia levels and L-Asp activity before the first dose and 24 h after the last dose of L-Asp, during each cycle of treatment. Plasma ammonia levels were analyzed immediately and 1 h after incubation at room temperature, and ex vivo ammonia production was defined as the increase in ammonia concentration. Ex vivo ammonia production correlated with L-Asp activity (R2 = 0.741), and ammonia levels measured immediately after blood collection were moderately correlated with L-Asp activity (R2 = 0.709). One patient with extranodal NK/T-cell lymphoma showed an increase in ammonia levels during the first cycle, but no increase in ammonia levels or L-Asp activity after L-Asp administration during the second cycle. Both ex vivo and in vivo ammonia production and surrogate markers are used for L-Asp biological activity.
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Metadaten
Titel
Correlation of ex vivo and in vivo ammonia production with L-asparaginase biological activity in adults with lymphoid malignancies
verfasst von
Takayuki Nakamura
Shuki Oya
Hidetoshi Ozawa
Yoshimi Maehiro
Seiya Muta
Masahiro Umeda
Yusuke Takaki
Toshinobu Fukuyama
Yoshitaka Yamasaki
Maki Yamaguchi
Kazutoshi Aoyama
Fumihiko Mouri
Yoshiki Naito
Koji Nagafuji
Publikationsdatum
16.02.2024
Verlag
Springer Nature Singapore
Erschienen in
International Journal of Hematology / Ausgabe 4/2024
Print ISSN: 0925-5710
Elektronische ISSN: 1865-3774
DOI
https://doi.org/10.1007/s12185-024-03718-y

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