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Erschienen in: Lasers in Medical Science 9/2020

06.02.2020 | Original Article

Determination of cadmium induced acute and chronic reproductive toxicity with Raman spectroscopy

verfasst von: Disheng Liu, Jianghou Wan, Zhenzhen Liu, Zhiyu Zhao, Guangru Zhang, Yufang Leng

Erschienen in: Lasers in Medical Science | Ausgabe 9/2020

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Abstract

Cadmium (Cd) is one of the toxic heavy metals which is confirmed to be related to male sterile. Here, confocal Raman spectroscopy was employed to detect biomolecular composition and changes in testis under acute and chronic Cd treatment. Specific Raman shifts associated with mitochondria, nucleic acids, proteins, lipids, and cholesterol were identified which were distinguishing among groups undergoing different Cd treatment times. Supporting evidences were provided by conventional experimental detections. The relevant biochemical parameters, pathological changes, and protein expression related to testosterone synthesis were all changed and consistent with Raman spectrum information. In conclusion, confocal Raman spectroscopy presents a reliable data and provides a novel method which is expected to be a promising strategy in reproduction toxicity research.
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Metadaten
Titel
Determination of cadmium induced acute and chronic reproductive toxicity with Raman spectroscopy
verfasst von
Disheng Liu
Jianghou Wan
Zhenzhen Liu
Zhiyu Zhao
Guangru Zhang
Yufang Leng
Publikationsdatum
06.02.2020
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 9/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-02976-6