Diagnosis of female 17α-hydroxylase deficiency after gonadectomy: a case report

17α-Hydroxylase is an enzyme that converts pregnenolone and progesterone to 17α-hydroxypregnenolone and 17α-hydroxyprogesterone, which are the precursors of sex steroids and cortisol (Fig. 1). 17α-Hydroxylase deficiency (17OHD) is a recessively inherited autosomal disease caused by mutations in the CYP17A1 gene [1]. It is a rare disease and accounts for approximately 1% of congenital adrenal cortex hyperplasias [2]. Patients with 17OHD typically have low blood levels of cortisol, estrogen, and androgens and a compensatory high blood level of adrenocorticotropic hormone (ACTH) [3]. Excessive levels of ACTH stimulate the production of 11-deoxycorticosterone and corticosterone, which have considerable mineralocorticoid activity, leading to extracellular volume expansion, hypertension, and hypokalemia [2, 3]. Deficiency of sex steroids causes primary amenorrhea in women [3] and feminization of external genitalia in men [4]. We report a case of a 29-year-old woman who had undergone gonadectomy in her childhood due to complete androgen insensitivity syndrome (AIS) and was diagnosed with 17OHD in adulthood.

An 11-year-old Japanese girl was transferred to the University of Tsukuba Hospital for inspective examination for ambiguous genitalia. From visual inspection of the external genital area, a raised lesion with pigmentation and plicae that looked like fused labia were noted. Behind the lesion, there was an external urethral opening without an inlet for a vagina. At the time of consultation, the patient’s serum estradiol level was less than 8 pg/ml, serum testosterone level was less than 0.5 ng/dl, serum follicle-stimulating hormone (FSH) level was 5.6 mIU/ml, and serum luteinizing hormone (LH) level was 2.8 mIU/ml. Magnetic resonance imaging showed no uterine corpus or vagina in the pelvis and revealed small tumors in the bilateral inguinal position that looked like the testes. The patient’s karyotype was determined as 46,XY, without any electrolyte abnormality. She had no siblings, and her parents were both Japanese. There was also no family history of hypertension or electrolyte abnormalities.

Gonadectomy and episioplasty were performed on the basis of diagnosis of complete AIS. At the time of admission, the patient’s blood pressure was 111–116 mmHg systolic and 60–70 mmHg diastolic. The histopathological findings of the resected gonads revealed maturation arrest of the spermatogonia with no malignant lesion. The patient had continually taken conjugated estrogens since her gonadectomy.

After 10 years, the patient was transferred to our hospital for vaginoplasty. She had no electrolyte abnormality, and her blood pressure was 150–160 mmHg systolic and 100–110 mmHg diastolic at the time of admission. Therefore, the vaginoplasty was performed when she was 21 years old.

After 4 years of the vaginoplasty, the patient had started to take a hypotensive drug (nifedipine 10 mg/day); at the age of 28 years, she presented with hypokalemia complicated with hypertension. Therefore, she was hospitalized at Mito Kyodo General Hospital for a detailed examination. The patient’s hormone profile and the results of each loading test are shown in Tables 1 and 2. The results were indicative of primary aldosteronism. Abdominal computed tomography demonstrated a bilateral adrenal gland swelling. Because 17OHD was suspected, further evaluation and appropriate genetic counseling were recommended.

Three months later, the patient and her family came to our hospital; after genetic counseling, the patient underwent the genetic test for the CYP17A1 gene using multiplex PCR and targeted next-generation sequencing as described previously [5]. The genetic analysis showed a compound heterozygous deletion of phenylalanine (Phe) codon in exon 1 [c.160_162delTTC (p.Phe54del)] and heterozygous mutation in exon 6 [c.1118A>T (p.His373Leu)]. These genetic variations were reported by Miura et al. in Japan [6] and Kim et al. in Korea [3]. The composite CYP17A1 mutant gene could be the cause of her disease. The genetic analysis of the patient’s mother revealed heterozygous mutation of exon 6 (c.1118A>T) and was acknowledged as the gene carrier. We could not perform genetic analysis of the patient’s father, because he could not be contacted at the time of testing, as her parents had divorced when she was a child. The blood pressure of the patient was controlled by a selective aldosterone blocker (eplerenone 50 mg/day) because she only showed symptoms of hyperaldosteronism and never showed any symptoms from the lower level of glucocorticoids, such as hypotension and dehydration. Fortunately, our patient did not have gender dysphoria identified socially as a female, and both she and her family accepted the results of the genetic examination.

Congenital adrenal hyperplasia (CAH) is an autosomal recessive disorder due to a defect in any of the enzymes mediating steroidogenesis. 17OHD is a rare type of CAH that causes cortisol and sex hormone deficiency and aldosterone excess. The most common cause of CAH is 21-hydroxylase deficiency (21OHD), and neonatal screening programs for CAH are designed to detect only 21OHD [7]. It is reported that patients with 17OHD are usually diagnosed by the anomaly of external genitalia at birth or in childhood [8], lack of sexual development, or hypertension in puberty [2, 6, 9, 10]. The clinician whose practice involves young patients with hypertension and primary amenorrhea should be aware of CAH as a differential diagnosis. However, when the phenotypic abnormality is mild, as in our patient’s case, such cases are more likely to be diagnosed in adulthood [11, 12].

Our patient did not have hypertension or hypokalemia at the age of 11 years. Although the levels of FSH and LH suggested the onset of puberty, the level of sex steroids was low, which can be considered as an indicator of differential diagnosis. Additionally, various load testing techniques (captopril suppressing test, saline loading test, and furosemide stress test) should be considered to confirm primary aldosteronism even when the patients are normotensive.

Disorders of sex development (DSD) with 46,XY have an abdominal gonad that presents a high risk of tumor development (about 35%) [13]. However, the risk of tubular in situ neoplasia is reported to increase later in life, and ultrasonography can help in the identification of the gonads once their precise position is known [13]. Therefore, recent guidelines recommend gonadectomy after puberty [14]. In this case, the genetic examination should have been considered at the age of 11 years because the result would have been useful in determining an indication for gonadectomy and other necessary treatments. Furthermore, we must consider the gender identity of the patient. Fortunately, the current patient did not have gender dysphoria and identified socially as female, and both the patient and her family accepted the results of the genetic examination.

The purposes of the diagnosis of DSD, including genetic examinations, are to avoid errors in the identification of acute adrenal insufficiency, which is potentially life-threatening, and to predict the issues that may present, such as hypertension, obesity, and insulin resistance. For precise individual care, a multidisciplinary team that includes not only a gynecologist but also a pediatric endocrine specialist, a clinical geneticist, and a genetic counselor should perform physical phenotyping and hormonal examination along with the genetic testing [15].

In conclusion, the differential diagnosis of DSD may be difficult when the patient is younger without complete phenotypes. However, because a late diagnosis could result in different treatment and prognosis, a genetic examination should be considered. In addition, a multidisciplinary team should be involved in the patient’s treatment and in communication with the patient and the family.