nach oben

Lasers in Medical Science

Erschienen in:

09.07.2020 | Original Article

Effect of photobiomodulation on CCC-ESF reactive oxygen species steady-state in high glucose mediums

verfasst von: Hongli Chen, Mengru Tu, Jia Shi, Yunhao Wang, Zhenhao Hou, Jinhai Wang

Erschienen in: Lasers in Medical Science | Ausgabe 3/2021

Einloggen, um Zugang zu erhalten

Abstract

Delayed wound healing is one of the most challenging complications of diabetes mellitus (DM) in clinical medicine, and it is related to the excessive generation of reactive oxygen species (ROS). Photobiomodulation (PBM) can promote wound healing in many ways, so it can be used as a method for the treatment of delayed healing of DM wounds. In this study, we investigated the effect of PBM on ROS homeostasis in human embryonic skin fibroblast cells (CCC-ESFs) cultured in high glucose concentrations. The CCC-ESFs were cultured in vitro and divided into two groups, including the control group and the 635 nm laser irradiation group. After 2 days of high glucose treatment, the experimental group was irradiated with different doses of laser for 3 days. First, we measured the cellular proliferation, and the results showed that laser irradiation could promote cellular proliferation. Then, we measured the generation of ROS, the activities of total superoxide dismutase (SOD), and total antioxidant capacity (TAC) of the cells; the results showed that high glucose destroyed cells by inducing high concentration of ROS, the balance of oxidation, and antioxidation cause oxidative stress damage to cells. PBM can increase the antioxidant capacity of cells, reducing the high concentration of ROS induced by high glucose. Finally, we measured the levels of mitochondrial membrane potential (∆ψ_m) and the secretion of nuclear factor kappa-B (NF-κB), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β); the results showed that PBM can reduce apoptosis and regulate the inflammatory state. We conclude that PBM can maintain the ROS homeostasis, increase the TAC of cells, and trigger the cellular proliferation, and the response of CCC-ESFs to PBM was dose-dependent.

Zaccardi O, Webb DR, Yates T et al (2016) Pathophysiology of type 1 and type 2 diabetes mellitus: a 90-year perspective. Post Grad Med J 92(1084):63–69. https://doi.org/10.1136/postgradmedj-2015-133281CrossRef

Suhariningsih, Winarni D, Husen SA et al (2019) The effect of electric field, magnetic field, and infrared ray combination to reduce HOMA-IR index and GLUT 4 in diabetic model of Mus musculus. Lasers Med Sci:1–7. https://doi.org/10.1007/s10103-019-02916-z

Arya AK, Tripathi K, Das P et al (2014) Promising role of ANGPTL4 gene in diabetic wound healing. Int J Low Extrem Wounds 13(1):58–63. https://doi.org/10.1177/1534734614520704CrossRefPubMed

Moura LI, Dias AM, Carvalho E et al (2013) Recent advances on the development of wound dressings for diabetic foot ulcer treatment-a review. Acta Biomater 9(7):7093–7114. https://doi.org/10.1016/j.actbio.2013.03.033CrossRefPubMed

Jazwa A, Kucharzewska P, Leja J et al (2010) Combined vascular endothelial growth factor-A and fibroblast growth factor 4 gene transfer improves wound healing in diabetic mice. Genet Vaccines Ther 8(1):6. https://doi.org/10.1186/1479-0556-8-6CrossRefPubMedPubMedCentral

Zecha JA, Raber-Durlacher JE, Nair RG et al (2016) Low level laser therapy/photobiomodulation in the management of side effects of chemoradiation therapy in head and neck cancer: part 1: mechanisms of action, dosimetric, and safety considerations. Support Care Cancer 24(6):2781–2792. https://doi.org/10.1007/s00520-016-3152-zCrossRefPubMedPubMedCentral

Chung H, Dai T, Sharma SK et al (2012) The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 40(2):516–533. https://doi.org/10.1007/s10439-011-0454-7CrossRefPubMed

Houreld NN, Sekhejane PR, Abrahamse H (2010) Irradiation at 830 nm stimulates nitric oxide production and inhibits pro-inflammatory cytokines in diabetic wounded fibroblast cells. Lasers Surg Med 42(6):494–502. https://doi.org/10.1002/lsm.20812CrossRefPubMed

Peng F, Zheng YD, Xu XQ et al (2011) 620 nm red light enhances osteogenic differentiation in mesenchymal stem cells. Acta Laser Biology Sinica 20(3):285–288. https://doi.org/10.1007/s10570-010-9464-0CrossRef

10.

Santos NR, dos Santos JN, dos Reis JA et al (2010) Influence of the use of laser phototherapy (lambda660 or 790 nm) on the survival of cutaneous flaps on diabetic rats. Photomed Laser Surg 28(4):483–488. https://doi.org/10.1089/pho.2009.2500CrossRefPubMed

11.

Martins DF, Turnes BL, Cidral-Filho FJ et al (2016) Light-emitting diode therapy reduces persistent inflammatory pain: role of interleukin 10 and antioxidant enzymes. Neuroscience 324:485–495. https://doi.org/10.1016/j.neuroscience.2016.03.035CrossRefPubMed

12.

Ye J, Wang S, Leonard SS et al (1999) Role of reactive oxygen species and p53 in chromium(VI)-induced apoptosis. J Biol Chem 274(49):34974–34980. https://doi.org/10.1074/jbc.274.49.34974CrossRefPubMed

13.

Cossarizza A (1993) A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′3,3′-tetraethylbenzimidazolcar-bocyanin iodide (JC-1). Biochem Biophys Res Commun 30(1):40–45. https://doi.org/10.1006/bbrc.1993.2438CrossRef

14.

Houreld NN, Abrahamse H (2008) Laser light influences cellular viability and proliferation in diabetic-wounded fibroblast cells in a dose- and wavelength-dependent manner. Lasers Med Sci 23(1):11–18. https://doi.org/10.1007/s10103-007-0445-yCrossRefPubMed

15.

Houreld N, Abrahamse H (2010) Low-intensity laser irradiation stimulates wound healing in diabetic wounded fibroblast cells (WS1). Diabetes Technol Ther 12(12):971–978. https://doi.org/10.1089/dia.2010.0039CrossRefPubMed

16.

Mirzaei M, Bayat M, Mosafa N et al (2007) Effect of low-level laser therapy on skin fibroblasts of streptozotocin-diabetic rats. Photomed Laser Surg 25(6):519–525. https://doi.org/10.1089/pho.2007.2098CrossRefPubMed

17.

Spitler R, Berns MW (2014) Comparison of laser and diode sources for acceleration of in vitro wound healing by low-level light therapy. J Biomed Opt 19(3):38001. https://doi.org/10.1117/1.JBO.19.3.038001CrossRefPubMed

18.

Chen HL, Wu HJ, Yin HJ et al (2019) Effect of photobiomodulation on neural differentiation of human umbilical cord mesenchymal stem cells. Lasers Med Sci 34(4):667–675. https://doi.org/10.1007/s10103-018-2638-yCrossRefPubMed

19.

Daíse RM, Verônica FA, Barbisan F et al (2019) In vitro effect of low-level laser therapy on the proliferative, apoptosis modulation, and oxi-inflammatory markers of premature-senescent hydrogen peroxide-induced dermal fibroblasts. Lasers Med Sci 34(11):1333–1343. https://doi.org/10.1007/s10103-019-02728-1CrossRef

20.

Panahi G, Pasalar P, Zare M et al (2018) MCU-knockdown attenuates high glucose-induced inflammation through regulating MAPKs/NF-kappaB pathways and ROS production in HepG2 cells. PLoS One 13(4):e0196580. https://doi.org/10.1371/journal.pone.0196580CrossRefPubMedPubMedCentral

21.

Bajaj S, Khan A (2012) Antioxidants and diabetes. Indian J Endocrinol Metab 16(Suppl 2):267–271. https://doi.org/10.4103/2230-8210.104057CrossRef

22.

Rahimi-Madiseh M, Malekpour-Tehrani A, Bahmani M et al (2016) The research and development on the antioxidants in prevention of diabetic complications. Asian Pac J TropMed 9(9):825–831. https://doi.org/10.1016/j.apjtm.2016.07.001CrossRef

23.

Dillenburg CS, Almeida LO, Martins MD et al (2014) Laser phototherapy triggers the production of reactive oxygen species in oral epithelial cells without inducing DNA damage. J Biomed Opt 19(4):048002. https://doi.org/10.1117/1.jbo.19.4.048002CrossRefPubMed

24.

Koracevic D, Koracevic G, Djordjevic V et al (2001) Method for the measurement of antioxidant activity in human. J Clin Pathol 54(5):356–361. https://doi.org/10.1136/jcp.54.5.356CrossRefPubMedPubMedCentral

25.

Chen HL, Wang H, Li Y et al (2016) Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells. AIP Adv 6(4):045018. https://doi.org/10.1063/1.4948442CrossRef

26.

Dolgova D, Abakumova T, Gening T et al (2019) Anti-inflammatory and cell proliferative effect of the 1270 nm laser irradiation on the BALB/c nude mouse model involves activation of the cell antioxidant system. Biomed Opt Express 10(8):4261–4275. https://doi.org/10.1364/BOE.10.004261CrossRefPubMedPubMedCentral

27.

Chen W, Yang J, Chen SH et al (2017) Importance of mitochondrial calcium uniporter in high glucose-induced endothelial cell dysfunction. Diab Vasc Dis Res 14(6):494–501. https://doi.org/10.1177/1479164117723270CrossRefPubMed

28.

Cho M, Hunt TK, Hussain MZ (2001) Hydrogen peroxide stimulates macrophage vascular endothelial growth factor release. Am J Physiol Heart Circ Physiol 280(5):H2357–H2363. https://doi.org/10.1152/ajpheart.2001.280.5.H2357CrossRefPubMed

29.

De Freitas LF, Hamblin MR (2016) Proposed mechanisms of photobiomodulation or low-level light therapy. IEEE J Sel Top Quantum Electron 22(3):1–17. https://doi.org/10.1109/JSTQE.2016.2561201CrossRef

30.

Carroll JD, Milward MR, Cooper PR et al (2014) Developments in low level light therapy (LLLT) for dentistry. Dent Mater 30(5):465–475. https://doi.org/10.1016/j.dental.2014.02.006CrossRefPubMed

31.

Brandner JM, Zacheja S, Houdek P et al (2008) Expression of matrix metalloproteinases, cytokines, and onnexins in diabetic and nondiabetic human keratinocytes before and after transplantation into an ex vivo wound-healing model. Diabetes Care 31(1):114–120. https://doi.org/10.2337/dc07-1304CrossRefPubMed

32.

Sekhejane PR, Houreld NN, Abrahamse H (2011) Irradiation at 636 nm positively affects diabetic wounded and hypoxic cells in vitro. Photomed Laser Surg 29(8):521–530. https://doi.org/10.1089/pho.2010.2877CrossRefPubMed

33.

Lee KD, Chiang MH, Chen PH et al (2018) The effect of low-level laser irradiation on hyperglycemia-induced inflammation in human gingival fibroblasts. Lasers Med Sci 34(5):913–920. https://doi.org/10.1007/s10103-018-2675-6CrossRefPubMed

34.

Svobodova B, Kloudova A, Ruzicka J et al (2019) The effect of 808 nm and 905 nm wavelength light on recovery after spinal cord injury. Sci Rep 9(1):7660–7674. https://doi.org/10.1038/s41598-019-44141-2CrossRefPubMedPubMedCentral

Titel: Effect of photobiomodulation on CCC-ESF reactive oxygen species steady-state in high glucose mediums
verfasst von: Hongli Chen
Mengru Tu
Jia Shi
Yunhao Wang
Zhenhao Hou
Jinhai Wang
Publikationsdatum: 09.07.2020
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 3/2021
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03057-4

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2021

Thermal field and tissue damage analysis of moving laser in cancer thermal therapy

The effect of various media and probe angles on the power output of the Cyclo G6 Glaucoma Laser System

Evaluating the efficacy and safety of vascular IPL for treatment of acute cutaneous leishmaniasis: a randomized controlled trial

Effects of photobiomodulation on cellular viability and cancer stem cell phenotype in oral squamous cell carcinoma

Dual-wavelength fiber-optic technique to assist needle cricothyroidotomy

Efficacy of non-ablative fractional 1440-nm laser therapy for treatment of facial acne scars in patients with rosacea: a prospective, interventional study