Skip to main content
main-content
Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie 5/2021

15.12.2020 | Original article

Expression of biological markers in gingival crevicular fluid of teeth with orthodontically induced root resorption

verfasst von: Kholoud Ahmed Abo Mandour, Marwa Ali Tawfeek, Mona A. Montasser

Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Ausgabe 5/2021

Einloggen, um Zugang zu erhalten

Abstract

Aim

The goal was to investigate interleukin‑1 receptor antagonist (IL-1ra) and dentin sialophosphoprotein (DSPP) levels in gingival crevicular fluid (GCF) as potential biomarkers for orthodontically induced root resorption (OIRR) using enzyme-linked immunosorbent immunoassay (ELISA).

Materials and methods

In all, 74 subjects were assigned to one of three groups: (1) orthodontic group included orthodontic patients who showed radiographic evidence of 1–3 mm root resorption of a maxillary central incisor, (2) pediatric group included pediatric patients who showed radiographic evidence of physiologic root resorption of a lower second primary molar, and (3) control group included subjects who had no orthodontic treatment and showed no radiographic evidence of root resorption. Samples from the GCF were collected with endodontic absorbent paper points inserted 1 mm below the gingival margin of the tooth. The IL-1ra and DSPP levels were evaluated using ELISA.

Results

The IL-1ra levels were 657.5 ± 51.5, 319.9 ± 181.3, and 129.4 ± 54.9 pg/ml for the control, orthodontic, and pediatric groups, respectively. The DSPP levels were 1.6 ± 1.0, 30.1 ± 9.6, and 39.2 ± 3.3 pg/ml for the control, orthodontic, and pediatric groups, respectively. Post hoc analyses revealed significant differences for IL-1ra and DSPP between any two groups. Sensitivity and specificity of IL-1ra for the diagnosis of OIRR showed 80% reliability and a cutoff value of ≤432.6 pg/ml, while the analysis of DSPP showed 100% reliability and a cutoff value of ≥7.33 pg/ml.

Conclusions

The levels of IL-1ra and DSPP detected in the orthodontic and pediatric groups indicate a possible association with OIRR. Efforts to develop tests for screening, diagnosis, and monitoring OIRR based on biological markers should continue.
Literatur
1.
Zurück zum Zitat Brezniak N, Wasserstein A (1993) Root resorption after orthodontic treatment: Part 1. Literature review. Am J Orthod Dentofacial Orthop 103:62–66 CrossRef Brezniak N, Wasserstein A (1993) Root resorption after orthodontic treatment: Part 1. Literature review. Am J Orthod Dentofacial Orthop 103:62–66 CrossRef
2.
Zurück zum Zitat Brezniak N, Wasserstein A (2002) Orthodontically induced inflammatory root resorption. Part I: the basic science aspects. Angle Orthod 72:175–179 PubMed Brezniak N, Wasserstein A (2002) Orthodontically induced inflammatory root resorption. Part I: the basic science aspects. Angle Orthod 72:175–179 PubMed
3.
Zurück zum Zitat Dudic A, Giannopoulou C, Leuzinger M, Kiliaridis S (2009) Detection of apical root resorption after orthodontic treatment by using panoramic radiography and cone-beam computed tomography of super-high resolution. Am J Orthod Dentofacial Orthop 135:434–437 CrossRef Dudic A, Giannopoulou C, Leuzinger M, Kiliaridis S (2009) Detection of apical root resorption after orthodontic treatment by using panoramic radiography and cone-beam computed tomography of super-high resolution. Am J Orthod Dentofacial Orthop 135:434–437 CrossRef
4.
Zurück zum Zitat Gegler A, Fontanella V (2008) In vitro evaluation of a method for obtaining periapical radiographs for diagnosis of external apical root resorption. Eur J Orthod 30:315–319 CrossRef Gegler A, Fontanella V (2008) In vitro evaluation of a method for obtaining periapical radiographs for diagnosis of external apical root resorption. Eur J Orthod 30:315–319 CrossRef
5.
Zurück zum Zitat Sameshima GT, Asgarifar KO (2001) Assessment of root resorption and root shape: periapical vs panoramic films. Angle Orthod 71:185–189 PubMed Sameshima GT, Asgarifar KO (2001) Assessment of root resorption and root shape: periapical vs panoramic films. Angle Orthod 71:185–189 PubMed
6.
Zurück zum Zitat Ren H, Chen J, Deng F, Zheng L, Liu X, Dong Y (2013) Comparison of cone-beam computed tomography and periapical radiography for detecting simulated apical root resorption. Angle Orthod 83:189–195 CrossRef Ren H, Chen J, Deng F, Zheng L, Liu X, Dong Y (2013) Comparison of cone-beam computed tomography and periapical radiography for detecting simulated apical root resorption. Angle Orthod 83:189–195 CrossRef
7.
Zurück zum Zitat Rody WJ Jr, Holliday LS, McHugh KP, Wallet SM, Spicer V, Krokhin O (2014) Mass spectrometry analysis of gingival crevicular fluid in the presence of external root resorption. Am J Orthod Dentofacial Orthop 145:787–798 CrossRef Rody WJ Jr, Holliday LS, McHugh KP, Wallet SM, Spicer V, Krokhin O (2014) Mass spectrometry analysis of gingival crevicular fluid in the presence of external root resorption. Am J Orthod Dentofacial Orthop 145:787–798 CrossRef
8.
Zurück zum Zitat Kereshanan S, Stephenson P, Waddington R (2008) Identification of dentine sialoprotein in gingival crevicular fluid during physiological root resorption and orthodontic tooth movement. Eur J Orthod 30:307–314 CrossRef Kereshanan S, Stephenson P, Waddington R (2008) Identification of dentine sialoprotein in gingival crevicular fluid during physiological root resorption and orthodontic tooth movement. Eur J Orthod 30:307–314 CrossRef
9.
Zurück zum Zitat Rody WJ Jr, Wijegunasinghe M, Holliday LS, McHugh KP, Wallet SM (2016) Immunoassay analysis of proteins in gingival crevicular fluid samples from resorbing teeth. Angle Orthod 86:187–192 CrossRef Rody WJ Jr, Wijegunasinghe M, Holliday LS, McHugh KP, Wallet SM (2016) Immunoassay analysis of proteins in gingival crevicular fluid samples from resorbing teeth. Angle Orthod 86:187–192 CrossRef
10.
Zurück zum Zitat Balducci L, Ramachandran A, Hao J, Narayanan K, Evans C, George A (2007) Biological markers for evaluation of root resorption. Arch Oral Biol 52:203–208 CrossRef Balducci L, Ramachandran A, Hao J, Narayanan K, Evans C, George A (2007) Biological markers for evaluation of root resorption. Arch Oral Biol 52:203–208 CrossRef
11.
Zurück zum Zitat Mah J, Prasad N (2004) Dentine phosphoproteins in gingival crevicular fluid during root resorption. Eur J Orthod 26:25–30 CrossRef Mah J, Prasad N (2004) Dentine phosphoproteins in gingival crevicular fluid during root resorption. Eur J Orthod 26:25–30 CrossRef
12.
Zurück zum Zitat Dimuzio M, Veis A (1978) Phosphophoryns—major noncollagenous proteins of rat incisor dentin. Calcif Tissue Res 25:169–178 CrossRef Dimuzio M, Veis A (1978) Phosphophoryns—major noncollagenous proteins of rat incisor dentin. Calcif Tissue Res 25:169–178 CrossRef
13.
Zurück zum Zitat Butler WT, Ritchie H (1995) The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol 39:169–179 PubMed Butler WT, Ritchie H (1995) The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol 39:169–179 PubMed
14.
Zurück zum Zitat Ritchie HH, Wang L‑H (1996) Sequence determination of an extremely acidic rat dentin phosphoprotein. J Biol Chem 271:21695–21698 CrossRef Ritchie HH, Wang L‑H (1996) Sequence determination of an extremely acidic rat dentin phosphoprotein. J Biol Chem 271:21695–21698 CrossRef
15.
Zurück zum Zitat Krishnan V, Davidovitch Z (2006) Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop 129(469):e1–e32 Krishnan V, Davidovitch Z (2006) Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop 129(469):e1–e32
16.
Zurück zum Zitat Wise G, King G (2008) Mechanisms of tooth eruption and orthodontic tooth movement. J Dent Res 87:414–434 CrossRef Wise G, King G (2008) Mechanisms of tooth eruption and orthodontic tooth movement. J Dent Res 87:414–434 CrossRef
17.
Zurück zum Zitat Ren Y, Vissink A (2008) Cytokines in crevicular fluid and orthodontic tooth movement. Eur J Oral Sci 116:89–97 CrossRef Ren Y, Vissink A (2008) Cytokines in crevicular fluid and orthodontic tooth movement. Eur J Oral Sci 116:89–97 CrossRef
18.
Zurück zum Zitat Arend WP (2002) The balance between IL‑1 and IL-1Ra in disease. Cytokine Growth Factor Rev 13:323–340 CrossRef Arend WP (2002) The balance between IL‑1 and IL-1Ra in disease. Cytokine Growth Factor Rev 13:323–340 CrossRef
19.
Zurück zum Zitat Moltó A, Olivé A (2010) Anti-IL‑1 molecules: new comers and new indications. Joint Bone Spine 77:102–107 CrossRef Moltó A, Olivé A (2010) Anti-IL‑1 molecules: new comers and new indications. Joint Bone Spine 77:102–107 CrossRef
20.
Zurück zum Zitat Svenson M, Nedergaard S, Heegaard PM, Whisenand TD, Arend WP, Bendtzen K (1995) Differential binding of human interleukin‑1 (IL-1) receptor antagonist to natural and recombinant soluble and cellular IL‑1 type I receptors. Eur J Immunol 25:2842–2850 CrossRef Svenson M, Nedergaard S, Heegaard PM, Whisenand TD, Arend WP, Bendtzen K (1995) Differential binding of human interleukin‑1 (IL-1) receptor antagonist to natural and recombinant soluble and cellular IL‑1 type I receptors. Eur J Immunol 25:2842–2850 CrossRef
21.
Zurück zum Zitat Cohen J (1988) Statistical power analysis for the behavioral sciences, 2nd edn. Lawrence Erlbaum, New York Cohen J (1988) Statistical power analysis for the behavioral sciences, 2nd edn. Lawrence Erlbaum, New York
22.
Zurück zum Zitat Faul F, Erdfelder E, Lang A‑G, Buchner A (2007) G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 39:175–191 CrossRef Faul F, Erdfelder E, Lang A‑G, Buchner A (2007) G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 39:175–191 CrossRef
23.
Zurück zum Zitat Krieger E, Drechsler T, Schmidtmann I, Jacobs C, Haag S, Wehrbein HJH et al (2013) Apical root resorption during orthodontic treatment with aligners? A retrospective radiometric study. Head Face Med 9:21 CrossRef Krieger E, Drechsler T, Schmidtmann I, Jacobs C, Haag S, Wehrbein HJH et al (2013) Apical root resorption during orthodontic treatment with aligners? A retrospective radiometric study. Head Face Med 9:21 CrossRef
24.
Zurück zum Zitat Fritz U, Diedrich P, Wiechmann D (2003) Apical root resorption after lingual orthodontic therapy. J Orofac Orthop 64:434–442 CrossRef Fritz U, Diedrich P, Wiechmann D (2003) Apical root resorption after lingual orthodontic therapy. J Orofac Orthop 64:434–442 CrossRef
25.
Zurück zum Zitat Akobeng AK (2007) Understanding diagnostic tests 1: sensitivity, specificity and predictive values. Acta Paediatr 96(3):338–341 CrossRef Akobeng AK (2007) Understanding diagnostic tests 1: sensitivity, specificity and predictive values. Acta Paediatr 96(3):338–341 CrossRef
26.
Zurück zum Zitat Lund H, Gröndahl K, Hansen K, Gröndahl HG (2012) Apical root resorption during orthodontic treatment. A prospective study using cone beam. Angle Orthod 82:480–487 CrossRef Lund H, Gröndahl K, Hansen K, Gröndahl HG (2012) Apical root resorption during orthodontic treatment. A prospective study using cone beam. Angle Orthod 82:480–487 CrossRef
27.
Zurück zum Zitat Killiany DM (1999) Root resorption caused by orthodontic treatment: an evidence-based review of literature. Semin Orthod 5:128–133 CrossRef Killiany DM (1999) Root resorption caused by orthodontic treatment: an evidence-based review of literature. Semin Orthod 5:128–133 CrossRef
28.
Zurück zum Zitat Topkara A, Karaman AI, Kau CH (2012) Apical root resorption caused by orthodontic forces: A brief review and a long-term observation. Eur J Dent 6:445–453 CrossRef Topkara A, Karaman AI, Kau CH (2012) Apical root resorption caused by orthodontic forces: A brief review and a long-term observation. Eur J Dent 6:445–453 CrossRef
30.
Zurück zum Zitat Sha H, Bai Y, Li S, Wang X, Yin Y (2014) Comparison between electrochemical ELISA and spectrophotometric ELISA for the detection of dentine sialophosphoprotein for root resorption. Am J Orthod Dentofacial Orthop 145(1):36–40 CrossRef Sha H, Bai Y, Li S, Wang X, Yin Y (2014) Comparison between electrochemical ELISA and spectrophotometric ELISA for the detection of dentine sialophosphoprotein for root resorption. Am J Orthod Dentofacial Orthop 145(1):36–40 CrossRef
31.
Zurück zum Zitat Lupi JE, Handelman CS, Sadowsky C (1996) Prevalence and severity of apical root resorption and alveolar bone loss in orthodontically treated adults. Am J Orthod Dentofacial Orthop 109(1):28–37 CrossRef Lupi JE, Handelman CS, Sadowsky C (1996) Prevalence and severity of apical root resorption and alveolar bone loss in orthodontically treated adults. Am J Orthod Dentofacial Orthop 109(1):28–37 CrossRef
Metadaten
Titel
Expression of biological markers in gingival crevicular fluid of teeth with orthodontically induced root resorption
verfasst von
Kholoud Ahmed Abo Mandour
Marwa Ali Tawfeek
Mona A. Montasser
Publikationsdatum
15.12.2020
Verlag
Springer Medizin
Erschienen in
Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Ausgabe 5/2021
Print ISSN: 1434-5293
Elektronische ISSN: 1615-6714
DOI
https://doi.org/10.1007/s00056-020-00267-x