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Erschienen in: Lasers in Medical Science 8/2021

03.01.2021 | Original Article

Fractionated carbon dioxide (CO2) laser treatment contributes to trans-nail penetration of rhodamine B and changes of cytokine microenvironment

verfasst von: Xiu-Hao Guan, Tian-Hua Xu, Xi Chen, Qi-Shuang Mu, Jing-Fei Suo, Rui-Xue Xu, John Chen, Ting Xiao, Gao Xing-Hua, Hong-Duo Chen

Erschienen in: Lasers in Medical Science | Ausgabe 8/2021

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Abstract

This study is to determine the role of the fractional CO2 laser in topical drug delivery and the impact of local immune responses. Experimental rabbit nails were treated with fractionated CO2 laser at varied fluencies of 20 mJ, 25 mJ, and 30 mJ and half of which were coated with rhodamine B (RhB). Histological examination was performed by hematoxylin and eosin staining; the penetration of RhB was assessed by the use of confocal laser scanning microscopy; and the expressions of IFN-γ and IL-4 mRNA in situ were detected by means of qPCR at 12 h, 24 h, 3 days, and 7 days post-laser irritation. The fractional CO2 laser could generate microscopic treatment zones in nail plates, and the depths of these micropores as well as the permeation of RhB in nails increased significantly in an energy-dependent manner. Importantly, the laser irritation led an upregulation of local IFN-γ mRNA expression accompanied by a downregulation of IL-4 mRNA expression. The ultrapulsed ablative fractionated CO2 laser may assist topical drug delivery, and may drive stronger local Th1 responses due to an imbalance of IFN-γ/IL-4 expressions, suggesting that the combination of ablative fractionated CO2 laser with topical agents would be an effective option for the treatment of onychomycosis.
Literatur
1.
Zurück zum Zitat Ghannoum MA, Hajjeh RA, Scher R, Konnikov N et al (2000) A large-scale North American study of fungal isolates from nails: the frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns. J Am Acad Dermatol 43:641–648 CrossRef Ghannoum MA, Hajjeh RA, Scher R, Konnikov N et al (2000) A large-scale North American study of fungal isolates from nails: the frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns. J Am Acad Dermatol 43:641–648 CrossRef
2.
Zurück zum Zitat Gupta AK, Jain HC, Lynde CW, Macdonald P et al (2000) Prevalence and epidemiology of onychomycosis in patients visiting physicians’ offices: a multicenter Canadian survey of 15,000 patients. J Am Acad Dermatol 43:244–248 CrossRef Gupta AK, Jain HC, Lynde CW, Macdonald P et al (2000) Prevalence and epidemiology of onychomycosis in patients visiting physicians’ offices: a multicenter Canadian survey of 15,000 patients. J Am Acad Dermatol 43:244–248 CrossRef
3.
Zurück zum Zitat Welsh O, Vera-Cabrera L, Welsh E (2010) Onychomycosis. Clin Dermatol 28:151–159 CrossRef Welsh O, Vera-Cabrera L, Welsh E (2010) Onychomycosis. Clin Dermatol 28:151–159 CrossRef
4.
Zurück zum Zitat Fujimoto T, Wang J, Baba K, Oki Y et al (2016) Transcutaneous drug delivery by liposomes using fractional laser technology. Lasers Surg Med 49:525–532 CrossRef Fujimoto T, Wang J, Baba K, Oki Y et al (2016) Transcutaneous drug delivery by liposomes using fractional laser technology. Lasers Surg Med 49:525–532 CrossRef
5.
Zurück zum Zitat de Oliveira GB, Antonio JR, Antonio CR, Tomé FA (2015) The association of fractional CO2 laser 10.600nm and photodynamic therapy in the treatment of onychomycosis. An Bras Dermatol 90:468–471 CrossRef de Oliveira GB, Antonio JR, Antonio CR, Tomé FA (2015) The association of fractional CO2 laser 10.600nm and photodynamic therapy in the treatment of onychomycosis. An Bras Dermatol 90:468–471 CrossRef
6.
Zurück zum Zitat Shi J, Li J, Huang H, Permatasari F et al (2017) The efficacy of fractional carbon dioxide (CO2) laser combined with terbinafine hydrochloride 1% cream for the treatment of onychomycosis. J Cosmet Laser Ther 19:353–359 CrossRef Shi J, Li J, Huang H, Permatasari F et al (2017) The efficacy of fractional carbon dioxide (CO2) laser combined with terbinafine hydrochloride 1% cream for the treatment of onychomycosis. J Cosmet Laser Ther 19:353–359 CrossRef
7.
Zurück zum Zitat Bhatta AK, Keyal U, Huang X, Zhao JJ (2016) Fractional carbon-dioxide (CO2) laser assisted topical therapy for the treatment of onychomycosis. J Am Acad Dermatol 74:916–923 CrossRef Bhatta AK, Keyal U, Huang X, Zhao JJ (2016) Fractional carbon-dioxide (CO2) laser assisted topical therapy for the treatment of onychomycosis. J Am Acad Dermatol 74:916–923 CrossRef
8.
Zurück zum Zitat Brem J (1981) Effective topical method of therapy for onychomycosis. Cutis 27:69 PubMed Brem J (1981) Effective topical method of therapy for onychomycosis. Cutis 27:69 PubMed
9.
Zurück zum Zitat Shemer A, Gupta AK, Amichai B, Farhi R et al (2016) An open comparative study of nail drilling as adjunctive treatment for toenail onychomycosis. J Dermatol Treat 27:480–483 CrossRef Shemer A, Gupta AK, Amichai B, Farhi R et al (2016) An open comparative study of nail drilling as adjunctive treatment for toenail onychomycosis. J Dermatol Treat 27:480–483 CrossRef
10.
Zurück zum Zitat Sumikawa M, Egawa T, Honda I, Yamamoto Y et al (2007) Effects of foot care intervention including nail drilling combined with topical antifungal application in diabetic patients with onychomyco- sis. J Dermatol 34:456–464 CrossRef Sumikawa M, Egawa T, Honda I, Yamamoto Y et al (2007) Effects of foot care intervention including nail drilling combined with topical antifungal application in diabetic patients with onychomyco- sis. J Dermatol 34:456–464 CrossRef
11.
Zurück zum Zitat Haedersdal M, Erlendsson AM, Paasch U, Anderson RR (2016) Translational medicine in the field of ablative fractional laser (AFXL)-assisted drug delivery: a critical review from basics to current clinical status. J Am Acad Dermatol 74:981–1004 CrossRef Haedersdal M, Erlendsson AM, Paasch U, Anderson RR (2016) Translational medicine in the field of ablative fractional laser (AFXL)-assisted drug delivery: a critical review from basics to current clinical status. J Am Acad Dermatol 74:981–1004 CrossRef
12.
Zurück zum Zitat Yoo KH, Kim BJ, Kim MN (2009) Enhanced efficacy of photodynamic therapy with methyl 5-aminolevulinic acid in recalcitrant periungual warts after ablative carbon dioxide fractional laser. Dermatol Surg 35:1927–1932 CrossRef Yoo KH, Kim BJ, Kim MN (2009) Enhanced efficacy of photodynamic therapy with methyl 5-aminolevulinic acid in recalcitrant periungual warts after ablative carbon dioxide fractional laser. Dermatol Surg 35:1927–1932 CrossRef
13.
Zurück zum Zitat Togsverd-Bo K, Lei U, Erlendsson AM, Taudorf EH et al (2015) Combination of ablative fractional laser and daylight-mediated photodynamic therapy for actinic keratosis in organ transplant recipients - a randomized controlled trial. Br J Dermatol 172:467–474 CrossRef Togsverd-Bo K, Lei U, Erlendsson AM, Taudorf EH et al (2015) Combination of ablative fractional laser and daylight-mediated photodynamic therapy for actinic keratosis in organ transplant recipients - a randomized controlled trial. Br J Dermatol 172:467–474 CrossRef
14.
Zurück zum Zitat Kim SK, Park J-Y, Song HS, Kim Y-S, Kim YC et al (2013) Photodynamic therapy with ablative carbon dioxide fractional laser for treating Bowen disease. Ann Dermatol 25:335–339 CrossRef Kim SK, Park J-Y, Song HS, Kim Y-S, Kim YC et al (2013) Photodynamic therapy with ablative carbon dioxide fractional laser for treating Bowen disease. Ann Dermatol 25:335–339 CrossRef
15.
Zurück zum Zitat Haak CS, Togsverd-Bo K, Thaysen-Petersen D, Wulf HC et al (2015) Fractional laser-mediated photodynamic therapy of high-risk basal cell carcinomas - a randomized clinical trial. Br J Dermatol 172:215–222 CrossRef Haak CS, Togsverd-Bo K, Thaysen-Petersen D, Wulf HC et al (2015) Fractional laser-mediated photodynamic therapy of high-risk basal cell carcinomas - a randomized clinical trial. Br J Dermatol 172:215–222 CrossRef
16.
Zurück zum Zitat Choi SH, Kim KH, Song KH (2015) Efficacy of ablative fractional laser-assisted photodynamic therapy for the treatment of actinic cheilitis: 12-month follow-up results of a prospective, randomized, comparative trial. Br J Dermatol 173:184–191 Choi SH, Kim KH, Song KH (2015) Efficacy of ablative fractional laser-assisted photodynamic therapy for the treatment of actinic cheilitis: 12-month follow-up results of a prospective, randomized, comparative trial. Br J Dermatol 173:184–191
17.
Zurück zum Zitat Waibel JS, Wulkan AJ, Shumaker PR (2013) Treatment of hypertrophic scars using laser and laser assisted corticosteroid delivery. Lasers Surg Med 453:135–140 CrossRef Waibel JS, Wulkan AJ, Shumaker PR (2013) Treatment of hypertrophic scars using laser and laser assisted corticosteroid delivery. Lasers Surg Med 453:135–140 CrossRef
18.
Zurück zum Zitat Lim EH, Kim HR, Park YO, Lee Y et al (2014) Toenail onychomycosis treated with a fractional carbon-dioxide laser and topical antifungal cream. J Am Acad Dermatol 705:918–923 CrossRef Lim EH, Kim HR, Park YO, Lee Y et al (2014) Toenail onychomycosis treated with a fractional carbon-dioxide laser and topical antifungal cream. J Am Acad Dermatol 705:918–923 CrossRef
19.
Zurück zum Zitat Zhou BR, Lu Y, Permatasari F, Huang H et al (2016) The efficacy of fractional carbon dioxide (CO2) laser combined with luliconazole 1% cream for the treatment of onychomycosis. Medicine(Baltimore) 95:e5141–e5148 Zhou BR, Lu Y, Permatasari F, Huang H et al (2016) The efficacy of fractional carbon dioxide (CO2) laser combined with luliconazole 1% cream for the treatment of onychomycosis. Medicine(Baltimore) 95:e5141–e5148
20.
Zurück zum Zitat Watanabe S, Kishida H, Okubo A (2017) Efficacy and safety of luliconazole 5% nail solution for the treatment of onychomycosis: a multicenter, double-blind, randomized phase III study. J Dermatol 447:753–759 CrossRef Watanabe S, Kishida H, Okubo A (2017) Efficacy and safety of luliconazole 5% nail solution for the treatment of onychomycosis: a multicenter, double-blind, randomized phase III study. J Dermatol 447:753–759 CrossRef
21.
Zurück zum Zitat Haedersdal M, Sakamoto FH, Farinelli WA, Doukas AG, Tam J, Anderson RR et al (2014) Pretreatment with ablative fractional laser changes kinetics and biodistribution of topical 5-aminolevulinic acid (ALA) and methyl aminolevulinate (MAL). Lasers Surg Med 466:462–469 CrossRef Haedersdal M, Sakamoto FH, Farinelli WA, Doukas AG, Tam J, Anderson RR et al (2014) Pretreatment with ablative fractional laser changes kinetics and biodistribution of topical 5-aminolevulinic acid (ALA) and methyl aminolevulinate (MAL). Lasers Surg Med 466:462–469 CrossRef
22.
Zurück zum Zitat Taudorf EH, Lerche CM, Erlendsson AM et al (2016) Laser-assisted delivery of topical methotrexate-in vitro investigations. Lasers Surg Med 48:519–529 CrossRef Taudorf EH, Lerche CM, Erlendsson AM et al (2016) Laser-assisted delivery of topical methotrexate-in vitro investigations. Lasers Surg Med 48:519–529 CrossRef
23.
Zurück zum Zitat Hsiao CY, Sung HC, Hu S, Huang CH (2015) Fractional CO2 laser treatment to enhance skin permeation of tranexamic acid with minimal skin disruption. Dermatology (Basel) 230:269–275 CrossRef Hsiao CY, Sung HC, Hu S, Huang CH (2015) Fractional CO2 laser treatment to enhance skin permeation of tranexamic acid with minimal skin disruption. Dermatology (Basel) 230:269–275 CrossRef
24.
Zurück zum Zitat Erlendsson AM, Taudorf EH, Eriksson AH, Haak CS et al (2015) Ablative fractional laser alters biodistribution of ingenol mebutate in the skin. Arch Dermatol Res 307:515–522 CrossRef Erlendsson AM, Taudorf EH, Eriksson AH, Haak CS et al (2015) Ablative fractional laser alters biodistribution of ingenol mebutate in the skin. Arch Dermatol Res 307:515–522 CrossRef
25.
Zurück zum Zitat Gupta C, Das S, Ramachandran VG, Saha R et al (2016) Possible role of trichophytin antigen in inducing impaired immunological clearance of fungus in onychomycosis. Mycopathologia 181:247–251 CrossRef Gupta C, Das S, Ramachandran VG, Saha R et al (2016) Possible role of trichophytin antigen in inducing impaired immunological clearance of fungus in onychomycosis. Mycopathologia 181:247–251 CrossRef
26.
Zurück zum Zitat Koga T (2003) Immune response in dermatophytosis. Nihon Ishinkin Gakkai Zasshi 444:273–275 [in Japanese] CrossRef Koga T (2003) Immune response in dermatophytosis. Nihon Ishinkin Gakkai Zasshi 444:273–275 [in Japanese] CrossRef
Metadaten
Titel
Fractionated carbon dioxide (CO2) laser treatment contributes to trans-nail penetration of rhodamine B and changes of cytokine microenvironment
verfasst von
Xiu-Hao Guan
Tian-Hua Xu
Xi Chen
Qi-Shuang Mu
Jing-Fei Suo
Rui-Xue Xu
John Chen
Ting Xiao
Gao Xing-Hua
Hong-Duo Chen
Publikationsdatum
03.01.2021
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 8/2021
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-03232-7