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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Erschienen in:

07.12.2021 | Original Article

Impact of FGF1 on human periodontal ligament fibroblast growth, osteogenic differentiation and inflammatory reaction in vitro

verfasst von: Dr. Isabel Knaup, DDS, Judit Symmank, Asisa Bastian, Sabine Neuss, Thomas Pufe, Collin Jacobs, Michael Wolf

Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Sonderheft 1/2022

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Abstract

Purpose

To investigate in vitro the impact of fibroblast growth factor 1 (FGF1) in comparison to ascorbic acid (AscA) on human periodontal ligament fibroblast (HPdLF) growth, their osteogenic differentiation, and modulation of their inflammatory reaction to mechanical stress.

Methods

The influence of different concentrations of FGF1 (12.5–200 ng/mL) on growth and proliferation of HPdLF cells was analyzed over 20 days by counting cell numbers and the percentage of Ki67-positive cells. Quantitative expression analysis of genes encoding the osteogenic markers alkaline phosphatase (ALPL), Runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and osteopontin (OSP), as well as the fibroblast markers vimentin (VIM) and fibroblast-specific protein 1 (FSP1), was performed after 2 and 20 days of cultivation. Metabolic activity was determined by MTT assay. For comparison with AscA, 50 ng/mL FGF1 was used for stimulation for 2 and 20 days. Cell number, percentage of Ki67-positive cells, and expression of osteoblast- and fibroblast-specific genes were examined. Alkaline phosphatase activity was visualized by NBT/BCIP and calcium deposits were stained with alizarin red. Cytokine (IL‑6, IL‑8, COX2/PGE2) expression and secretion were analyzed by qPCR and ELISA in 6 h mechanically compressed HPdLF cultured for 2 days with FGF1 or ascorbic acid.

Results

Higher concentrations of FGF1 promoted cell proliferation upon short-term stimulation, whereas prolonged treatment induced the expression of osteogenic markers even with low concentrations. AscA promotes cell growth more markedly than FGF1 in short-term cultures, whereas FGF1 induced osteogenic cell fate more strongly in long-term culture. Both factors induced an increased inflammatory response of HPdLF to mechanical compression.

Conclusion

Our data suggest that FGF1 promotes an osteogenic phenotype of HPdLF and limits inflammatory response to mechanical forces compared to AscA.

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Titel: Impact of FGF1 on human periodontal ligament fibroblast growth, osteogenic differentiation and inflammatory reaction in vitro
verfasst von: Dr. Isabel Knaup, DDS
Judit Symmank
Asisa Bastian
Sabine Neuss
Thomas Pufe
Collin Jacobs
Michael Wolf
Publikationsdatum: 07.12.2021
Verlag: Springer Medizin
Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Ausgabe Sonderheft 1/2022
Print ISSN: 1434-5293
Elektronische ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-021-00363-6

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Impact of FGF1 on human periodontal ligament fibroblast growth, osteogenic differentiation and inflammatory reaction in vitro

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