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Erschienen in: Lasers in Dental Science 2/2021

06.05.2021 | Original Article

Inhibition of S. mutans after nanoparticle mediated photodynamic antimicrobial chemotherapy on oral biofilm flow-cell system using laser or LED

verfasst von: Kelly Maria Silva Moreira, Talita S. Graziano, Luciana Solera Sales, Felipe Joia, Rafael Nobrega Stipp, Regina Maria Puppin-Rontani, Marinês Nobre-dos-Santos, Lidiany Karla Azevedo Rodrigues, Carolina Steiner-Oliveira

Erschienen in: Lasers in Dental Science | Ausgabe 2/2021

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Abstract

Purpose

This study tested the effect of photodynamic antimicrobial chemotherapy (PACT) on single-species biofilm grown in a flow-cell system using methylene blue associated with nanoparticles of β-cyclodextrin and light sources of red laser or light-emitting diode (LED).

Methods

The flow-cell system comprised chambers for biofilm cultivation with S. mutans under continuous hydrodynamic conditions containing BHI supplemented with 1% sucrose (w/v). Biofilms were divided into six groups (n = 6): C (control), P (photosensitizer + β-cyclodextrin), L (laser), LED (light-emitting diode), LP (laser + photosensitizer + β-cyclodextrin), and LEDP (LED + photosensitizer + β-cyclodextrin). Groups irradiated with laser were exposed to a low power light (λ = 660 nm, 320 J/cm2, 0.1 W, 9 J, 90 s). Groups irradiated with LEDs were exposed to 12 LEDs (λ = 660 nm, 8.1 J/cm2, 0.09 W/cm2, and 8.1 J for 90 s). The antibacterial potential of treatments was assessed by viable S. mutans counts of biofilm in selective medium. The vitality of intact biofilms was qualitatively evaluated by confocal laser scanning microscopy analysis (CLSM). The data were analyzed by Welch’s one-way ANOVA followed by Scheffe´s post-hoc tests (p < 0.05).

Results

No reductions in the number of S. mutans for C, P, L, and LED groups were found (p > 0.05). The number of S. mutans was significantly reduced in LP and LEDP groups (p < 0.05) and LP had the lowest microbial numbers (p < 0.05).

Conclusion

PACT protocol was effective in reducing S. mutans in the dynamic biofilm model tested using laser or LED. Furthermore, laser as a light source presented a better performance than LED.
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Metadaten
Titel
Inhibition of S. mutans after nanoparticle mediated photodynamic antimicrobial chemotherapy on oral biofilm flow-cell system using laser or LED
verfasst von
Kelly Maria Silva Moreira
Talita S. Graziano
Luciana Solera Sales
Felipe Joia
Rafael Nobrega Stipp
Regina Maria Puppin-Rontani
Marinês Nobre-dos-Santos
Lidiany Karla Azevedo Rodrigues
Carolina Steiner-Oliveira
Publikationsdatum
06.05.2021
Verlag
Springer International Publishing
Erschienen in
Lasers in Dental Science / Ausgabe 2/2021
Elektronische ISSN: 2367-2587
DOI
https://doi.org/10.1007/s41547-021-00128-9

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