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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

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23.02.2023 | Original Article

Long-term changes in thickness, live/dead bacterial ratio, and mineral content in biofilm on ceramic and stainless steel orthodontic attachments

verfasst von: Anjali Krishnan, MDS, Rahul Rajendran, S MDS, Deepak Damodaran, R MDS, Sreelekshmi K. Manmadhan, MDS, Vinod Krishnan, MDS. M.Orth RCS FDS RCS PhD

Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Sonderheft 3/2023

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Abstract

Purpose

Fixed orthodontic appliances induce biofilm deposition, which harbors a microbial population harmful to the periodontal health of the individual. The present study evaluated the changes in thickness, live/dead bacterial ratio, and mineral content in dental biofilm over 6 months in patients with either stainless steel or ceramic orthodontic attachments.

Methods

Eighty patients who require fixed orthodontic appliance treatment with first premolar extraction for correcting their malocclusion were selected and bonded with either stainless steel or ceramic orthodontic attachments on the buccal side. The attached buttons were retrieved at different periods—1 week, 1 month, 3 months, and 6 months. They were stained and visualized through confocal microscopy to detect biofilm thickness and the ratio of live/dead bacteria. X‑ray diffraction was used to identify the presence of calcium and phosphorous.

Results

Ceramic attachments showed a greater increase in biofilm thickness in comparison to stainless steel attachments except in the initial 1‑week evaluation. A higher live/dead bacterial ratio was observed in stainless steel attachments than in their ceramic counterparts at all four evaluation periods. Both stainless steel and ceramic surfaces exhibited the presence of mineral deposition (calcium and phosphorous) at all periods.

Conclusions

More biofilm adhesion was observed over ceramic surfaces than over stainless steel orthodontic attachments. Stainless steel attachments exhibited biofilm with a higher live/dead bacterial ratio than their ceramic counterparts at all evaluation periods. The presence of calcium and phosphorous in the adhered biofilm, pointing toward its calcification process, was identified.

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Titel: Long-term changes in thickness, live/dead bacterial ratio, and mineral content in biofilm on ceramic and stainless steel orthodontic attachments
verfasst von: Anjali Krishnan, MDS
Rahul Rajendran, S MDS
Deepak Damodaran, R MDS
Sreelekshmi K. Manmadhan, MDS
Vinod Krishnan, MDS. M.Orth RCS FDS RCS PhD
Publikationsdatum: 23.02.2023
Verlag: Springer Medizin
Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Ausgabe Sonderheft 3/2023
Print ISSN: 1434-5293
Elektronische ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-023-00452-8

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Long-term changes in thickness, live/dead bacterial ratio, and mineral content in biofilm on ceramic and stainless steel orthodontic attachments

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

Purpose

Methods

Results

Conclusions

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